Tyrosine-protein kinase wzc (P76387)

Identification
Name:Tyrosine-protein kinase wzc
Synonyms:Not Available
Gene Name:wzc
Enzyme Class:Not Available
Biological Properties
General Function:Cell wall/membrane/envelope biogenesis
Specific Function:Required for the extracellular polysaccharide colanic acid synthesis. The autophosphorylated form is inactive. Probably involved in the export of colanic acid from the cell to medium. Phosphorylates udg
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Complex Reactions:
1.0Thumb+1.0a [protein]-L-tyrosine → 1.0Thumb+1.0a [protein]-L-tyrosine phosphate
1.0Adenosine triphosphate + 1.0a [protein]-L-tyrosine → 1.0ADP + 1.0a [protein]-L-tyrosine phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
GO Classification:
Component
cell part
membrane
Function
enzyme regulator activity
Process
capsule polysaccharide biosynthetic process
carbohydrate metabolic process
cellular polysaccharide biosynthetic process
extracellular polysaccharide biosynthetic process
lipid biosynthetic process
lipid metabolic process
lipopolysaccharide biosynthetic process
metabolic process
polysaccharide biosynthetic process
polysaccharide metabolic process
primary metabolic process
Gene Properties
Blattner:Not Available
Gene OrientationNot Available
Centisome Percentage:Not Available
Left Sequence EndNot Available
Right Sequence EndNot Available
Gene Sequence:
>2163 bp
ATGACAGAAAAAGTAAAACAACATGCCGCTCCGGTAACGGGCAGTGATGAAATCGATATT
GGTCGCCTGGTCGGCACCGTCATTGAAGCGCGCTGGTGGGTGATTGGCATCACCACCGTA
TTCGCCCTCTGTGCCGTGGTTTACACCTTCTTCGCCACGCCGATTTATAGTGCCGACGCA
CTGGTACAAATCGAGCAAAACAGCGGCAATTCGTTAGTGCAGGACATTGGTTCGGCATTA
GCCAACAAACCGCCTGCATCGGACGCCGAGATCCAGTTGATTCGTTCGCGTCTGGTGCTT
GGTAAAACGGTGGACGATCTCGACCTCGATATTGCAGTGAGCAAAAACACGTTCCCGATT
TTCGGTGCGGGCTGGGATCGCCTGATGGGACGTCAGAACGAGACGGTGAAAGTGACCACC
TTTAACCGCCCGAAAGAGATGGCGGATCAGGTGTTTACGCTTAATGTGCTGGACAACAAA
AACTACACCCTGAGCAGCGACGGCGGCTTTAGCGCCCGGGGGCAAGCGGGCCAGATGCTG
AAAAAAGAAGGCGTCACGCTGATGGTTGAAGCCATTCACGCCAGCCCGGGCAGTGAGTTT
ACCGTCACCAAATACTCCACGCTGGGGATGATCAACCAACTGCAAAACAGCCTGACGGTA
ACGGAGAACGGCAAAGACGCTGGCGTTTTGAGCCTGACTTATACCGGTGAAGATCGTGAA
CAGATCCGCGACATTCTTAACAGCATCGCCCGTAACTATCAGGAACAAAATATTGAGCGC
AAATCGGCGGAAGCGTCGAAAAGCCTCGCCTTCCTCGCGCAACAGTTACCGGAAGTACGT
AGCCGCTTGGATGTTGCCGAAAACAAACTGAATGCCTTCCGTCAGGATAAAGATTCTGTT
GATCTGCCGCTGGAAGCAAAAGCGGTGCTCGATTCGATGGTGAACATCGATGCGCAGTTG
AACGAACTGACCTTTAAAGAGGCGGAAATCTCCAAGCTGTACACCAAAGTTCACCCGGCG
TACCGCACGCTGCTGGAGAAACGTCAGGCGCTGGAAGACGAAAAAGCCAAACTTAACGGT
CGCGTAACGGCGATGCCGAAAACCCAGCAGGAGATTGTCCGTCTGACCCGCGATGTCGAG
TCTGGTCAGCAGGTCTATATGCAACTGCTGAATAAAGAGCAGGAGCTGAAAATCACCGAG
GCCAGCACCGTCGGCGATGTGCGCATTGTTGACCCGGCAATCACTCAGCCTGGCGTGCTG
AAACCGAAGAAAGGGCTGATTATCCTCGGCGCGATTATCCTTGGCCTGATGCTTTCTATC
GTGGGTGTGCTGCTGCGCTCGTTGTTTAATCGCGGCATTGAAAGCCCGCAAGTACTGGAA
GAACACGGTATTAGCGTCTATGCCAGCATCCCGCTGTCGGAATGGCAGAAAGCGCGTGAT
AGCGTCAAAACCATCAAAGGGATTAAACGCTATAAACAGAGCCAGCTACTGGCGGTGGGG
AATCCAACCGATCTGGCGATTGAAGCCATCCGTAGTCTGCGTACCAGTTTGCACTTCGCG
ATGATGCAGGCACAGAACAATGTGTTGATGATGACCGGGGTTAGCCCGTCAATTGGTAAA
ACCTTTGTCTGCGCCAACCTGGCGGCGGTGATCAGCCAGACCAATAAACGCGTGTTGTTG
ATCGACTGCGATATGCGCAAAGGCTACACCCACGAGCTGTTGGGCACTAATAACGTTAAT
GGCCTGTCGGAAATTCTGATTGGTCAGGGCGATATTACTACCGCTGCTAAACCGACCTCT
ATTGCCAAATTTGACCTGATCCCGCGCGGTCAGGTGCCGCCAAATCCTTCTGAACTGTTG
ATGAGCGAACGCTTTGCCGAACTGGTGAACTGGGCGAGTAAAAACTATGACCTGGTGTTG
ATTGATACGCCGCCGATTCTGGCAGTGACCGATGCGGCAATTGTTGGTCGTCATGTCGGA
ACCACGTTAATGGTGGCGCGTTATGCGGTCAACACATTGAAAGAAGTGGAAACCAGTCTG
AGCCGCTTTGAGCAAAACGGTATTCCGGTGAAAGGGGTGATTCTGAACTCCATCTTCCGC
CGCGCCAGCGCGTATCAGGATTATGGCTATTACGAATACGAATATAAGTCGGATGCGAAA
TAA
Protein Properties
Pfam Domain Function:
Protein Residues:720
Protein Molecular Weight:79342
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • 32-52
  • 425-445
Protein Sequence:
>Tyrosine-protein kinase wzc
MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITTVFALCAVVYTFFATPIYSADA
LVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGKTVDDLDLDIAVSKNTFPI
FGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDNKNYTLSSDGGFSARGQAGQML
KKEGVTLMVEAIHASPGSEFTVTKYSTLGMINQLQNSLTVTENGKDAGVLSLTYTGEDRE
QIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQLPEVRSRLDVAENKLNAFRQDKDSV
DLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLYTKVHPAYRTLLEKRQALEDEKAKLNG
RVTAMPKTQQEIVRLTRDVESGQQVYMQLLNKEQELKITEASTVGDVRIVDPAITQPGVL
KPKKGLIILGAIILGLMLSIVGVLLRSLFNRGIESPQVLEEHGISVYASIPLSEWQKARD
SVKTIKGIKRYKQSQLLAVGNPTDLAIEAIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGK
TFVCANLAAVISQTNKRVLLIDCDMRKGYTHELLGTNNVNGLSEILIGQGDITTAAKPTS
IAKFDLIPRGQVPPNPSELLMSERFAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVG
TTLMVARYAVNTLKEVETSLSRFEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK
References
External Links:
ResourceLink
Uniprot ID:P76387
Uniprot Name:WZC_ECOLI
GenBank Gene ID:U00096
Genebank Protein ID:87082032
CCDB:WZC_ECOLI
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Grangeasse, C., Doublet, P., Cozzone, A. J. (2002). "Tyrosine phosphorylation of protein kinase Wzc from Escherichia coli K12 occurs through a two-step process." J Biol Chem 277:7127-7135. Pubmed: 11751920
  • Grangeasse, C., Obadia, B., Mijakovic, I., Deutscher, J., Cozzone, A. J., Doublet, P. (2003). "Autophosphorylation of the Escherichia coli protein kinase Wzc regulates tyrosine phosphorylation of Ugd, a UDP-glucose dehydrogenase." J Biol Chem 278:39323-39329. Pubmed: 12851388
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Itoh, T., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Kasai, H., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Seki, Y., Horiuchi, T., et, a. l. .. (1996). "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." DNA Res 3:379-392. Pubmed: 9097040
  • Stevenson, G., Andrianopoulos, K., Hobbs, M., Reeves, P. R. (1996). "Organization of the Escherichia coli K-12 gene cluster responsible for production of the extracellular polysaccharide colanic acid." J Bacteriol 178:4885-4893. Pubmed: 8759852
  • Vincent, C., Doublet, P., Grangeasse, C., Vaganay, E., Cozzone, A. J., Duclos, B. (1999). "Cells of Escherichia coli contain a protein-tyrosine kinase, Wzc, and a phosphotyrosine-protein phosphatase, Wzb." J Bacteriol 181:3472-3477. Pubmed: 10348860
  • Vincent, C., Duclos, B., Grangeasse, C., Vaganay, E., Riberty, M., Cozzone, A. J., Doublet, P. (2000). "Relationship between exopolysaccharide production and protein-tyrosine phosphorylation in gram-negative bacteria." J Mol Biol 304:311-321. Pubmed: 11090276