Chain length determinant protein (P76372)

Identification
Name:Chain length determinant protein
Synonyms:
  • Polysaccharide antigen chain regulator
Gene Name:wzzB
Enzyme Class:Not Available
Biological Properties
General Function:Involved in lipopolysaccharide biosynthetic process
Specific Function:Confers a modal distribution of chain length on the O- antigen component of lipopolysaccharide (LPS). Gives rise to a reduced number of short chain molecules and increases in numbers of longer molecules
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Complex Reactions:
1.0(O16 antigen)x2 undecaprenyl diphosphate+1.0Thumb → 1.0Thumb+1.0(O16 antigen)x3 undecaprenyl diphosphate+1.0Thumb
1.0(O16 antigen)x2 undecaprenyl diphosphate + 1.0O16 antigen undecaprenyl diphosphate → 1.0Hydrogen ion + 1.0(O16 antigen)x3 undecaprenyl diphosphate + 1.0Undecaprenyl diphosphate
ReactionCard
1.0(O16 antigen)x3 undecaprenyl diphosphate+1.0Thumb → 1.0Thumb+1.0(O16 antigen)x4 undecaprenyl diphosphate+1.0Thumb
1.0(O16 antigen)x3 undecaprenyl diphosphate + 1.0O16 antigen undecaprenyl diphosphate → 1.0Hydrogen ion + 1.0(O16 antigen)x4 undecaprenyl diphosphate + 1.0Undecaprenyl diphosphate
ReactionCard
2.0Thumb → 1.0Thumb+1.0(O16 antigen)x2 undecaprenyl diphosphate+1.0Thumb
2.0O16 antigen undecaprenyl diphosphate → 1.0Hydrogen ion + 1.0(O16 antigen)x2 undecaprenyl diphosphate + 1.0Undecaprenyl diphosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB21225Hydrogen ionMetaboCard
ECMDB21257O16 antigen undecaprenyl diphosphateMetaboCard
ECMDB01469Undecaprenyl diphosphateMetaboCard
GO Classification:
Component
cell part
membrane
Process
lipid biosynthetic process
lipid metabolic process
lipopolysaccharide biosynthetic process
metabolic process
primary metabolic process
Gene Properties
Blattner:b2027
Gene OrientationCounterclockwise
Centisome Percentage:45.16
Left Sequence End2095345
Right Sequence End2096325
Gene Sequence:
>981 bp
ATGAGTCATCAACTTACCTTCGCCGACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGA
AAAGAGATTTTCTTGTCCCGCATGGAGCAGATTCTGCCATGGCAAAACATGGTGGAAGTC
ATCGAGCCGTTTTACCCCAAGGCTGGTAATGGCCGGCGACCTTATCCGCTGGAAACCATG
CTACGCATTCACTGCATGCAGCATTGGTACAACCTGAGCGATGGCGCGATGGAAGATGCT
CTGTACGAAATCGCCTCCATGCGTCTGTTTGCCCGGTTATCCCTGGATAGCGCCTTGCCG
GACCGCACCACCATCATGAATTTCCGCCACCTGCTGGAGCAGCATCAACTGGCCCGCCAA
TTGTTCAAGACCATCAATCGCTGGCTGGCCGAAGCAGGCGTCATGATGACTCAAGGCACC
TTGGTCGATGCCACCATCATTGAGGCACCCAGCTCGACCAAGAACAAAGAGCAGCAACGC
GATCCGGAGATGCATCAGACCAAGAAAGGCAATCAGTGGCACTTTGGCATGAAGGCCCAC
ATTGGTGTCGATGCCAAGAGTGGCCTGACCCACAGCCTGGTCACCACCGCGGCCAACGAG
CATGACCTCAATCAGCTGGGTAATCTGCTGCATGGAGAGGAGCAATTTGTCTCAGCCGAT
GCCGGCTACCAAGGGGCGCCACAGCGCGAGGAGCTGGCCGAGGTGGATGTGGACTGGCTG
ATCGCCGAGCGCCCCGGCAAGGTAAGAACCTTGAAACAGCATCCACGCAAGAACAAAACG
GCCATCAACATCGAATACATGAAAGCCAGCATCCGGGCCAGGGTGGAGCACCCATTTCGC
ATCATCAAGCGACAGTTCGGCTTCGTGAAAGCCAGATACAAGGGGTTGCTGAAAAACGAT
AACCAACTGGCGATGTTATTCACGCTGGCCAACCTGTTTCGGGCGGACCAAATGATACGT
CAGTGGGAGAGATCTCACTAA
Protein Properties
Pfam Domain Function:
Protein Residues:326
Protein Molecular Weight:36455
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • 32-52
  • 296-316
Protein Sequence:
>Chain length determinant protein
MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIALAIGYLAVAKEKWTSTA
IITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSAFSALAETLDNQEEREKLT
IEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVNQELEKDLKDNIALGRKNLQD
SLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQIQQTGEDITQDTLFLLGSEALESM
IKHEATRPLVFSPNYYQTRQNLLDIESLKVDDLDIHAYRYVMKPMLPIRRDSPKKAITLI
LAVLLGGMVGAGIVLGRNALRNYNAK
References
External Links:
ResourceLink
Uniprot ID:P76372
Uniprot Name:WZZB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674726
Ecogene ID:EG13406
Ecocyc:EG13406
ColiBase:b2027
Kegg Gene:b2027
EchoBASE ID:EB3182
CCDB:WZZB_ECOLI
BacMap:90111375
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Itoh, T., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Kasai, H., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Seki, Y., Horiuchi, T., et, a. l. .. (1996). "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." DNA Res 3:379-392. Pubmed: 9097040
  • Stenberg, F., Chovanec, P., Maslen, S. L., Robinson, C. V., Ilag, L. L., von Heijne, G., Daley, D. O. (2005). "Protein complexes of the Escherichia coli cell envelope." J Biol Chem 280:34409-34419. Pubmed: 16079137