Succinylglutamate desuccinylase (P76215)

Identification
Name:Succinylglutamate desuccinylase
Synonyms:Not Available
Gene Name:astE
Enzyme Class:
Biological Properties
General Function:Involved in hydrolase activity, acting on ester bonds
Specific Function:Transforms N(2)-succinylglutamate into succinate and glutamate
Cellular Location:Cytoplasmic
SMPDB Pathways:
KEGG Pathways:
  • Arginine and proline metabolism ec00330
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Water + 1.0N-Succinyl-L-glutamate ↔ 1.0L-Glutamate + 1.0Succinic acid
ReactionCard
SMPDB Reactions:
1.0 N2-succinylglutamate+1.0Thumb+1.0Thumb → 1.0L-Glutamic acid+1.0Thumb+1.0Thumb
1.0 N2-succinylglutamate + 1.0Water + 1.0 N2-succinylglutamate → 1.0L-Glutamic acid + 1.0Succinic acid + 1.0L-Glutamate
ReactionCard
EcoCyc Reactions:
1.0N2-succinylglutamate+1.0Thumb → 1.0Thumb+1.0Thumb
1.0N2-succinylglutamate + 1.0Water → 1.0Succinic acid + 1.0L-Glutamate
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0N-Succinyl-L-glutamate + 1.0Water → 1.0Succinic acid + 1.0L-Glutamate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB24191 N2-succinylglutamateMetaboCard
ECMDB00148L-GlutamateMetaboCard
ECMDB01437N-Succinyl-L-glutamateMetaboCard
ECMDB00254Succinic acidMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
binding
catalytic activity
cation binding
hydrolase activity
hydrolase activity, acting on ester bonds
ion binding
metal ion binding
transition metal ion binding
zinc ion binding
Process
arginine catabolic process
arginine catabolic process to glutamate
arginine metabolic process
cellular amino acid and derivative metabolic process
cellular amino acid metabolic process
cellular metabolic process
glutamine family amino acid metabolic process
metabolic process
Gene Properties
Blattner:b1744
Gene OrientationCounterclockwise
Centisome Percentage:39.31
Left Sequence End1823979
Right Sequence End1824947
Gene Sequence:
>969 bp
ATGGCTCAGCTTTCGTTACAACATATTCAAAAAATCTACGATAACCAGGTGCATGTGGTG
AAGGACTTCAACCTGGAAATTGCCGATAAAGAGTTCATCGTGTTTGTCGGCCCGTCGGGC
TGCGGTAAGTCGACCACCCTGCGCATGATTGCCGGGCTTGAGGAGATCAGCGGCGGCGAT
CTGTTGATCGACGGCAAACGAATGAATGACGTTCCAGCCAAAGCACGCAATATAGCGATG
GTGTTCCAGAACTACGCGTTGTATCCGCATATGACGGTTTACGACAACATGGCGTTTGGT
CTGAAGATGCAAAAAATCGCCAAAGAGGTGATTGATGAGCGGGTGAACTGGGCGGCGCAA
ATTCTCGGCCTGCGTGAGTACCTGAAACGTAAGCCGGGGGCGCTTTCCGGCGGGCAACGT
CAGCGAGTGGCGCTTGGGCGGGCGATTGTACGCGAAGCGGGCGTGTTTTTAATGGATGAA
CCGCTCTCTAACCTTGATGCCAAGCTGCGCGTGCAAATGCGCGCAGAGATCAGCAAGCTG
CATCAGAAACTGAACACCACCATGATCTACGTGACCCACGATCAGACCGAAGCGATGACC
ATGGCGACGCGGATTGTGATTATGAAAGACGGGATTGTTCAGCAAGTAGGTGCGCCGAAA
ACCGTTTATAACCAACCCGCGAATATGTTTGTTTCCGGATTTATTGGATCACCAGCGATG
AATTTTATTCGCGGCACGATCGATGGCGATAAATTCGTTACGGAAACGCTTAAATTAACC
ATTCCCGAAGAGAAATTAGCGGTTCTGAAAACACAGGAAAGTTTGCATAAGCCCATCGTG
ATGGGAATACGACCGGAAGATATTCATCCGGACGCGCAAGAGGAAAATAACATTTCCGCC
AAAATTAGCGTGGCAGAATTAACCGGTGCGGAATTTATGCTCTACACCACGGTTGGGGGC
ACGAGTTAG
Protein Properties
Pfam Domain Function:
Protein Residues:322
Protein Molecular Weight:35800
Protein Theoretical pI:7
PDB File:1YW6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Succinylglutamate desuccinylase
MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQGALVISAGIHGNETAPV
EMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDMNRMFGGRWQLFAESGETC
RARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGVLPQRDIPWDEKFLTWLGAAG
LEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFGQNDLRQFAVTASAIAALLSGES
VGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFMPFEKGTLLAQDGEERFTVTHDVEY
VLFPNPLVALGLRAGLMLEKIS
References
External Links:
ResourceLink
Uniprot ID:P76215
Uniprot Name:ASTE_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1742155
PDB ID:1YW6
Ecogene ID:EG13995
Ecocyc:EG13995
ColiBase:b1744
Kegg Gene:b1744
EchoBASE ID:EB3751
CCDB:ASTE_ECOLI
BacMap:16129698
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hagenmaier, S., Stierhof, Y. D., Henning, U. (1997). "A new periplasmic protein of Escherichia coli which is synthesized in spheroplasts but not in intact cells." J Bacteriol 179:2073-2076. Pubmed: 9068658
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Kiupakis, A. K., Reitzer, L. (2002). "ArgR-independent induction and ArgR-dependent superinduction of the astCADBE operon in Escherichia coli." J Bacteriol 184:2940-2950. Pubmed: 12003934
  • Makarova, K. S., Grishin, N. V. (1999). "The Zn-peptidase superfamily: functional convergence after evolutionary divergence." J Mol Biol 292:11-17. Pubmed: 10493853
  • Schneider, B. L., Kiupakis, A. K., Reitzer, L. J. (1998). "Arginine catabolism and the arginine succinyltransferase pathway in Escherichia coli." J Bacteriol 180:4278-4286. Pubmed: 9696779