Gamma-glutamyl-gamma-aminobutyrate hydrolase (P76038)

Identification
Name:Gamma-glutamyl-gamma-aminobutyrate hydrolase
Synonyms:
  • Gamma-Glu-GABA hydrolase
Gene Name:puuD
Enzyme Class:
Biological Properties
General Function:Involved in hydrolase activity
Specific Function:Involved in the breakdown of putrescine via hydrolysis of the gamma-glutamyl linkage of gamma-glutamyl-gamma- aminobutyrate
Cellular Location:Cytoplasmic
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.04-(Glutamylamino) butanoate + 1.0Water ↔ 1.0gamma-Aminobutyric acid + 1.0L-Glutamate
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb → 1.0L-Glutamic acid+1.0Thumb+1.0Thumb
1.04-(Glutamylamino) butanoate + 1.0Water → 1.0L-Glutamic acid + 1.0gamma-Aminobutyric acid + 1.0L-Glutamate
ReactionCard
1.0gamma-glutamyl-gamma-aminobutyrate+1.0Thumb → 1.0Thumb+1.0Thumb
1.0gamma-glutamyl-gamma-aminobutyrate + 1.0Water → 1.0gamma-Aminobutyric acid + 1.0L-Glutamate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.04-(Glutamylamino) butanoate + 1.0Water ↔ 1.0gamma-Aminobutyric acid + 1.0L-Glutamate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB121614-(Glutamylamino) butanoateMetaboCard
ECMDB04017gamma-Aminobutyric acidMetaboCard
ECMDB00148L-GlutamateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
catalytic activity
hydrolase activity
Process
cellular amino acid and derivative metabolic process
cellular amino acid metabolic process
cellular metabolic process
glutamine family amino acid metabolic process
glutamine metabolic process
metabolic process
Gene Properties
Blattner:b1298
Gene OrientationClockwise
Centisome Percentage:29.29
Left Sequence End1359144
Right Sequence End1359908
Gene Sequence:
>765 bp
ATGCAAGCATTGCTGGAACACTTTATTACCCAATCCACCGTGTATTCATTGATGGCGGTG
GTGTTGGTGGCCTTTCTGGAGTCGCTGGCGCTGGTCGGTTTGATTCTACCCGGTACGGTG
CTGATGGCGGGGCTGGGAGCGCTGATTGGCAGCGGCGAGTTAAGTTTCTGGCACGCCTGG
CTGGCAGGGATTATTGGCTGCTTGATGGGCGACTGGATTTCTTTCTGGCTGGGTTGGCGT
TTTAAAAAGCCGTTGCATCGCTGGTCATTTCTGAAGAAAAACAAAGCACTACTTGATAAA
ACTGAACATGCGTTGCATCAACACAGCATGTTCACCATTCTGGTCGGTCGTTTTGTTGGC
CCGACGCGTCCGCTGGTGCCAATGGTGGCGGGAATGCTGGATCTGCCGGTGGCTAAATTT
ATTACGCCGAATATTATCGGCTGCCTGCTGTGGCCGCCGTTTTACTTCCTGCCAGGGATT
CTGGCGGGCGCGGCGATCGATATTCCTGCCGGAATGCAGAGCGGTGAGTTTAAATGGTTG
CTGCTGGCAACAGCGGTGTTTTTGTGGGTTGGTGGCTGGCTGTGCTGGCGGTTATGGCGC
AGCGGTAAAGCGACTGACCGTTTGAGTCATTATTTGTCCCGCGGTCGTTTGTTGTGGCTG
ACGCCGTTGATTTCTGCCATCGGCGTGGTGGCGCTGGTGGTGTTAATTCGCCACCCGTTG
ATGCCGGTGTATATCGATATTTTGCGTAAAGTGGTTGGGGTTTAG
Protein Properties
Pfam Domain Function:
Protein Residues:254
Protein Molecular Weight:28013
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Gamma-glutamyl-gamma-aminobutyrate hydrolase
MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIALPHALAEPSLLEQLLP
KLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINAALERRIPIFAICRGLQEL
VVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQVEEGGLLSALLPECSNFWVN
SLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALGVQWHPEWNSSEYALSRILFEGF
ITACQHHIAEKQRL
References
External Links:
ResourceLink
Uniprot ID:P76038
Uniprot Name:PUUD_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674310
Ecogene ID:EG13909
Ecocyc:EG13909
ColiBase:b1298
Kegg Gene:b1298
EchoBASE ID:EB3668
CCDB:PUUD_ECOLI
BacMap:90111245
General Reference:
  • Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kasai, H., Kashimoto, K., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Horiuchi, T., et, a. l. .. (1996). "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." DNA Res 3:363-377. Pubmed: 9097039
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Kurihara, S., Oda, S., Kato, K., Kim, H. G., Koyanagi, T., Kumagai, H., Suzuki, H. (2005). "A novel putrescine utilization pathway involves gamma-glutamylated intermediates of Escherichia coli K-12." J Biol Chem 280:4602-4608. Pubmed: 15590624