Catecholate siderophore receptor fiu (P75780)

Identification
Name:Catecholate siderophore receptor fiu
Synonyms:
  • Ferric iron uptake protein
  • TonB-dependent receptor fiu
Gene Name:fiu
Enzyme Class:Not Available
Biological Properties
General Function:Involved in receptor activity
Specific Function:Involved in the active transport across the outer membrane of iron complexed with catecholate siderophores such as dihydroxybenzoylserine and dihydroxybenzoate. It derives its energy for transport by interacting with the trans-periplasmic membrane protein tonB. Can also transport catechol-substitued cephalosporins. Receptor for microcins M, H47 and E492
Cellular Location:Cell outer membrane
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Transports:
Transport References:
  • Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
Metabolites:
ECMDB IDNameView
ECMDB21331ferric 2,3-dihydroxybenzoylserineMetaboCard
ECMDB21225Hydrogen ionMetaboCard
GO Classification:
Component
cell part
membrane
Function
binding
cation binding
cation transmembrane transporter activity
di-, tri-valent inorganic cation transmembrane transporter activity
inorganic cation transmembrane transporter activity
ion binding
ion transmembrane transporter activity
iron ion binding
iron ion transmembrane transporter activity
metal ion binding
molecular transducer activity
receptor activity
siderophore-iron transmembrane transporter activity
signal transducer activity
substrate-specific transmembrane transporter activity
transition metal ion binding
transmembrane transporter activity
transporter activity
Process
establishment of localization
siderophore transport
transport
Gene Properties
Blattner:b0805
Gene OrientationCounterclockwise
Centisome Percentage:18.07
Left Sequence End838472
Right Sequence End840754
Gene Sequence:
>2283 bp
ATGGAAAACAATCGCAATTTCCCTGCCAGACAATTTCATTCGCTCACGTTCTTTGCCGGT
CTTTGTATTGGCATCACGCCTGTGGCTCAGGCACTCGCCGCCGAAGGGCAAACTAACGCG
GATGACACGCTGGTTGTCGAAGCATCAACGCCTTCGCTTTATGCGCCACAACAATCTGCC
GATCCGAAATTCTCGCGTCCGGTAGCGGATACTACCCGCACGATGACGGTAATTTCTGAA
CAAGTGATTAAAGATCAGGGCGCAACCAACCTTACCGACGCGCTCAAAAACGTCCCCGGC
GTGGGTGCGTTTTTTGCGGGTGAGAACGGTAACTCCACCACTGGCGACGCCATTTATATG
CGTGGTGCCGATACCTCTAACAGTATTTATATTGATGGCATTCGCGATATCGGCAGCGTC
TCGCGCGACACCTTCAATACCGAGCAGGTCGAAGTGATTAAAGGGCCGTCCGGCACCGAC
TACGGGCGCAGCGCACCGACAGGCTCGATCAATATGATCAGCAAGCAGCCGCGCAATGAT
TCCGGCATTGACGCCTCCGCCAGTATTGGCAGCGCCTGGTTCCGCCGCGGCACGCTGGAC
GTCAATCAGGTCATTGGTGATACCACCGCGGTGCGCCTGAATGTAATGGGCGAAAAAACG
CACGATGCCGGACGCGACAAAGTCAAAAATGAGCGTTACGGCGTCGCCCCTTCTGTCGCT
TTTGGCCTTGGTACAGCGAATCGTTTGTATCTTAATTATCTGCATGTCACCCAGCACAAC
ACGCCAGACGGCGGCATTCCGACCATCGGTTTGCCGGGCTATTCTGCCCCATCTGCGGGA
ACGGCGGCCCTGAATCATTCCGGAAAAGTTGATACTCATAACTTTTACGGCACGGATTCC
GATTACGACGATTCGACCACCGACACCGCCACCATGCGTTTTGAGCACGACATCAACGAT
AACACCACCATTCGCAATACTACCCGTTGGTCGCGCGTAAAGCAGGATTACCTGATGACG
GCGATTATGGGCGGGGCGTCGAATATTACTCAGCCCACCAGCGATGTGAATAGCTGGACC
TGGTCACGCACGGCGAATACCAAAGATGTGAGTAATAAAATTCTCACCAACCAGACCAAC
CTGACCTCGACGTTCTATACCGGTTCTATCGGTCATGATGTCAGTACCGGCGTGGAATTT
ACCCGTGAAACGCAGACTAACTACGGCGTTAATCCGGTGACGTTACCCGCGGTAAATATT
TATCATCCTGACAGCAGCATTCATCCCGGCGGCCTGACGCGCAACGGCGCAAACGCCAAT
GGTCAGACGGATACCTTCGCAATTTACGCCTTTGATACGCTGCAAATCACCCGTGATTTT
GAGCTGAACGGCGGGATCCGTCTGGATAATTATCATACTGAATATGACAGTGCCACCGCC
TGCGGCGGCAGCGGACGCGGTGCCATCACCTGCCCAACTGGTGTGGCAAAAGGTTCTCCG
GTCACCACCGTCGACACCGCCAAGTCGGGCAATCTGATGAACTGGAAAGCCGGGGCGCTG
TATCACCTGACGGAAAACGGCAATGTCTATATTAACTATGCCGTTTCCCAGCAGCCTCCG
GGCGGCAACAACTTCGCCCTTGCGCAGTCTGGCAGCGGTAACAGTGCCAACCGCACCGAT
TTTAAACCGCAAAAAGCCAACACCAGCGAGATTGGCACCAAATGGCAGGTTCTGGATAAA
CGTCTGTTGCTCACCGCCGCGCTGTTCCGCACTGATATCGAAAATGAAGTTGAGCAAAAT
GATGACGGAACTTACTCGCAATACGGTAAGAAACGCGTCGAAGGCTATGAGATATCCGTG
GCCGGGAATATCACTCCCGCGTGGCAGGTGATTGGCGGCTATACCCAGCAAAAAGCAACC
ATCAAAAACGGCAAAGATGTTGCCCAGGATGGTTCCTCATCGCTGCCGTATACCCCGGAG
CACGCCTTCACCTTATGGAGCCAATATCAGGCAACCGACGATATCTCTGTTGGCGCGGGC
GCACGCTATATCGGCAGTATGCATAAAGGTTCAGACGGCGCGGTGGGAACGCCAGCGTTT
ACCGAAGGTTACTGGGTCGCCGATGCCAAACTGGGGTATCGAGTTAATCGCAATCTCGAC
TTCCAGCTAAACGTTTACAACCTGTTTGATACCGATTACGTCGCCTCAATCAACAAGAGC
GGCTACCGTTATCACCCGGGCGAGCCAAGAACCTTCTTGCTCACAGCCAATATGCATTTC
TGA
Protein Properties
Pfam Domain Function:
Protein Residues:760
Protein Molecular Weight:81960
Protein Theoretical pI:6
Signaling Regions:
  • 1-31
Transmembrane Regions:
  • None
Protein Sequence:
>Catecholate siderophore receptor fiu
MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTLVVEASTPSLYAPQQSA
DPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVGAFFAGENGNSTTGDAIYM
RGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTDYGRSAPTGSINMISKQPRND
SGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGEKTHDAGRDKVKNERYGVAPSVA
FGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSAPSAGTAALNHSGKVDTHNFYGTDS
DYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVKQDYLMTAIMGGASNITQPTSDVNSWT
WSRTANTKDVSNKILTNQTNLTSTFYTGSIGHDVSTGVEFTRETQTNYGVNPVTLPAVNI
YHPDSSIHPGGLTRNGANANGQTDTFAIYAFDTLQITRDFELNGGIRLDNYHTEYDSATA
CGGSGRGAITCPTGVAKGSPVTTVDTAKSGNLMNWKAGALYHLTENGNVYINYAVSQQPP
GGNNFALAQSGSGNSANRTDFKPQKANTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQN
DDGTYSQYGKKRVEGYEISVAGNITPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPE
HAFTLWSQYQATDDISVGAGARYIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLD
FQLNVYNLFDTDYVASINKSGYRYHPGEPRTFLLTANMHF
References
External Links:
ResourceLink
Uniprot ID:P75780
Uniprot Name:FIU_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4062366
Ecogene ID:EG13317
Ecocyc:EG13317
ColiBase:b0805
Kegg Gene:b0805
EchoBASE ID:EB3101
CCDB:FIU_ECOLI
BacMap:16128773
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Curtis, N. A., Eisenstadt, R. L., East, S. J., Cornford, R. J., Walker, L. A., White, A. J. (1988). "Iron-regulated outer membrane proteins of Escherichia coli K-12 and mechanism of action of catechol-substituted cephalosporins." Antimicrob Agents Chemother 32:1879-1886. Pubmed: 3072926
  • Destoumieux-Garzon, D., Peduzzi, J., Thomas, X., Djediat, C., Rebuffat, S. (2006). "Parasitism of iron-siderophore receptors of Escherichia coli by the siderophore-peptide microcin E492m and its unmodified counterpart." Biometals 19:181-191. Pubmed: 16718603
  • Hantke, K. (1990). "Dihydroxybenzoylserine--a siderophore for E. coli." FEMS Microbiol Lett 55:5-8. Pubmed: 2139424
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Molloy, M. P., Herbert, B. R., Slade, M. B., Rabilloud, T., Nouwens, A. S., Williams, K. L., Gooley, A. A. (2000). "Proteomic analysis of the Escherichia coli outer membrane." Eur J Biochem 267:2871-2881. Pubmed: 10806384
  • Newman, D. L., Shapiro, J. A. (1999). "Differential fiu-lacZ fusion regulation linked to Escherichia coli colony development." Mol Microbiol 33:18-32. Pubmed: 10411720
  • Niehaus, F., Hantke, K., Unden, G. (1991). "Iron content and FNR-dependent gene regulation in Escherichia coli." FEMS Microbiol Lett 68:319-323. Pubmed: 1804764
  • Nikaido, H., Rosenberg, E. Y. (1990). "Cir and Fiu proteins in the outer membrane of Escherichia coli catalyze transport of monomeric catechols: study with beta-lactam antibiotics containing catechol and analogous groups." J Bacteriol 172:1361-1367. Pubmed: 2407721
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Patzer, S. I., Baquero, M. R., Bravo, D., Moreno, F., Hantke, K. (2003). "The colicin G, H and X determinants encode microcins M and H47, which might utilize the catecholate siderophore receptors FepA, Cir, Fiu and IroN." Microbiology 149:2557-2570. Pubmed: 12949180