Identification
Name:2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase
Synonyms:Not Available
Gene Name:ubiF
Enzyme Class:Not Available
Biological Properties
General Function:Involved in oxidoreductase activity
Specific Function:Oxygenase that introduces the hydroxyl group at carbon five of 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol resulting in the formation of 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4- benzoquinol
Cellular Location:Not Available
SMPDB Pathways:
  • Secondary Metabolites: Ubiquinol biosynthesis PW000981
  • Secondary Metabolites: Ubiquinol biosynthesis 2 PW002036
KEGG Pathways:
  • Ubiquinone and other terpenoid-quinone biosynthesis ec00130
KEGG Reactions:
1.02-Polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone+1.0Thumb+1.0Thumb+1.0Thumb1.02-Polyprenyl-3-methyl-5-hydroxy-6-methoxy-1,4-benzoquinone+1.0Thumb+1.0Thumb
1.02-Polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone + 1.0Oxygen + 1.0NADPH + 1.0Hydrogen ion ↔ 1.02-Polyprenyl-3-methyl-5-hydroxy-6-methoxy-1,4-benzoquinone + 1.0NADP + 1.0Water
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Reduced acceptor+1.0Thumb1.0Thumb+1.0oxidized electron acceptor+1.0Thumb
1.0Oxygen + 1.0Reduced acceptor + 1.06-Methoxy-3-methyl-2-all-trans-octaprenyl-1,4-benzoquinol → 1.0Water + 1.0oxidized electron acceptor + 1.03-demethylubiquinol-8
ReactionCard
1.02-Octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol +1.0Thumb+1.0Reduced acceptor1.02-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4-benzoquinone+1.0Thumb+1.0oxidized electron acceptor
1.02-Octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol + 1.0Oxygen + 1.0Reduced acceptor → 1.02-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4-benzoquinone + 1.0Water + 1.0oxidized electron acceptor
ReactionCard
Complex Reactions:
1.0Thumb+0.5Thumb1.0Thumb
Metabolites:
ECMDB IDNameView
ECMDB211562-Octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4-benzoquinolMetaboCard
ECMDB211572-Octaprenyl-3-methyl-6-methoxy-1,4-benzoquinolMetaboCard
ECMDB242593-demethylubiquinol-8MetaboCard
ECMDB234366-Methoxy-3-methyl-2-all-trans-octaprenyl-1,4-benzoquinolMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00217NADPMetaboCard
ECMDB04111NADPHMetaboCard
ECMDB04124OxygenMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
adenyl nucleotide binding
binding
catalytic activity
FAD or FADH2 binding
monooxygenase activity
nucleoside binding
oxidoreductase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NADH or NADPH as one donor, and incorporation of one atom of oxygen
purine nucleoside binding
Process
cellular metabolic process
coenzyme metabolic process
cofactor metabolic process
metabolic process
oxidation reduction
oxidoreduction coenzyme metabolic process
ubiquinone biosynthetic process
ubiquinone metabolic process
Gene Properties
Blattner:b0662
Gene OrientationClockwise
Centisome Percentage:14.96
Left Sequence End694324
Right Sequence End695499
Gene Sequence:
>1176 bp
ATGGATACGTCACTGGCTGAGGAAGTACAGCAGACCATGGCAACACTTGCGCCCAATCGC
TTTTTCTTTATGTCGCCGTACCGCAGTTTTACGACGTCAGGATGTTTCGCCCGCTTCGAT
GAACCGGCTGTGAACGGGGATTCGCCCGACAGTCCCTTCCAGCAAAAACTCGCCGCGCTG
TTTGCCGATGCCAAAGCGCAGGGCATCAAAAATCCGGTGATGGTCGGGGCGATTCCCTTC
GATCCACGTCAGCCTTCGTCGCTGTATATTCCTGAATCCTGGCAGTCGTTCTCCCGTCAG
GAAAAACAAGCTTCCGCACGCCGTTTCACCCGCAGCCAGTCGCTGAATGTGGTGGAACGC
CAGGCAATTCCGGAGCAAACCACGTTTGAACAGATGGTTGCCCGCGCCGCCGCACTTACC
GCCACGCCGCAGGTCGACAAAGTGGTGTTGTCACGGTTGATTGATATCACCACTGACGCC
GCCATTGATAGTGGCGTATTGCTGGAACGGTTGATTGCGCAAAACCCGGTTAGTTACAAC
TTCCATGTTCCGCTGGCTGATGGTGGCGTCCTGCTGGGGGCCAGCCCGGAACTGCTGCTA
CGTAAAGACGGCGAGCGTTTTAGCTCCATTCCGTTAGCCGGTTCCGCGCGTCGTCAGCCG
GATGAAGTGCTCGATCGCGAAGCAGGTAATCGTCTGCTGGCGTCAGAAAAAGATCGCCAT
GAACATGAACTGGTGACTCAGGCGATGAAAGAGGTACTGCGCGAACGCAGTAGTGAGTTA
CACGTTCCTTCTTCTCCACAGCTGATCACCACGCCGACGCTGTGGCATCTCGCAACTCCC
TTTGAAGGTAAAGCGAATTCGCAAGAAAACGCACTGACTCTGGCCTGTCTGCTGCATCCG
ACCCCCGCGCTGAGCGGTTTCCCGCATCAGGCCGCGACCCAGGTTATTGCTGAACTGGAA
CCGTTCGACCGCGAACTGTTTGGCGGCATTGTGGGTTGGTGTGACAGCGAAGGTAACGGC
GAATGGGTGGTGACCATCCGCTGCGCGAAGCTGCGGGAAAATCAGGTGCGTCTGTTTGCC
GGAGCGGGGATTGTGCCTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGTCAAA
CTTTCTACCATGTTGAACGTTTTTGGATTGCATTAA
Protein Properties
Pfam Domain Function:
Protein Residues:391
Protein Molecular Weight:42953
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol hydroxylase
MTNQPTEIAIVGGGMVGGALALGLAQHGFAVTVIEHAEPAPFVADSQPDVRISAISAASV
SLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKLPLLGYMVENTVLQQALWQ
ALEAHPKVTLRVPGSLIALHRHDDLQELELKGGEVIRAKLVIGADGANSQVRQMAGIGVH
AWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPLFDNWASLVWYDSPARIRQLQNM
NMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHALQYVQPGLALVGDAAHTIHPLAGQG
VNLGYRDVDALIDVLVNARSYGEAWASYPVLKRYQMRRMADNFIMQSGMDLFYAGFSNNL
PPLRFMRNLGLMAAERAGVLKRQALKYALGL
References
External Links:
ResourceLink
Uniprot ID:P75728
Uniprot Name:UBIF_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674714
Ecogene ID:EG13658
Ecocyc:EG13658
ColiBase:b0662
Kegg Gene:b0662
EchoBASE ID:EB3422
CCDB:UBIF_ECOLI
BacMap:16128645
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Kwon, O., Kotsakis, A., Meganathan, R. (2000). "Ubiquinone (coenzyme Q) biosynthesis in Escherichia coli: identification of the ubiF gene." FEMS Microbiol Lett 186:157-161. Pubmed: 10802164
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232