L-fucose isomerase (P69922)

Identification
Name:L-fucose isomerase
Synonyms:
  • 6-deoxy-L-galactose isomerase
  • FucIase
Gene Name:fucI
Enzyme Class:
Biological Properties
General Function:Involved in L-fucose isomerase activity
Specific Function:Converts the aldose L-fucose into the corresponding ketose L-fuculose. Is also able to convert D-arabinose into D- ribulose, but this isomerase has a higher affinity for fucose and fuculose than for arabinose and ribulose, respectively
Cellular Location:Cytoplasm
SMPDB Pathways:
KEGG Pathways:
  • Fructose and mannose metabolism ec00051
  • Microbial metabolism in diverse environments ec01120
KEGG Reactions:
1.0Thumb ↔ 1.0Thumb
1.0L-Fucose ↔ 1.0L-Fuculose
ReactionCard
SMPDB Reactions:
1.0Thumb → 1.0keto-L-rhamnulose
1.0alpha-L-Fucose → 1.0keto-L-rhamnulose
ReactionCard
1.0D-arabinopyranose ↔ 1.0Thumb
1.0D-arabinopyranose ↔ 1.0D-Ribulose
ReactionCard
Complex Reactions:
1.0Thumb → 1.0Thumb
1.0L-Fucose → 1.0L-Fuculose
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB20264alpha-L-FucoseMetaboCard
ECMDB00621D-RibuloseMetaboCard
ECMDB00174L-FucoseMetaboCard
ECMDB04069L-FuculoseMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
binding
catalytic activity
cation binding
intramolecular oxidoreductase activity
intramolecular oxidoreductase activity, interconverting aldoses and ketoses
ion binding
isomerase activity
L-fucose isomerase activity
manganese ion binding
metal ion binding
transition metal ion binding
Process
alcohol metabolic process
fucose catabolic process
fucose metabolic process
hexose metabolic process
metabolic process
monosaccharide metabolic process
small molecule metabolic process
Gene Properties
Blattner:b2802
Gene OrientationClockwise
Centisome Percentage:63.23
Left Sequence End2933606
Right Sequence End2935381
Gene Sequence:
>1776 bp
ATGAAAAAAATCAGCTTACCGAAAATTGGTATCCGCCCGGTTATTGACGGTCGTCGCATG
GGTGTTCGTGAGTCGCTTGAAGAACAAACAATGAATATGGCGAAAGCTACGGCCGCACTG
CTGACCGAGAAACTGCGCCATGCCTGCGGAGCTGCCGTCGAGTGTGTCATTTCCGATACC
TGTATCGCGGGTATGGCTGAAGCCGCTGCTTGCGAAGAAAAATTCAGCAGTCAGAATGTA
GGCCTCACCATTACGGTAACGCCTTGCTGGTGCTATGGCAGTGAAACCATCGACATGGAT
CCAACCCGCCCGAAGGCCATTTGGGGCTTTAACGGCACTGAACGCCCCGGCGCTGTTTAC
CTGGCAGCGGCTCTGGCAGCTCACAGCCAGAAAGGCATCCCAGCATTCTCCATTTACGGT
CATGACGTTCAGGATGCCGATGACACATCGATTCCTGCCGATGTTGAAGAAAAACTGCTG
CGCTTTGCCCGCGCCGGTTTGGCCGTCGCCAGCATGAAAGGTAAAAGCTATCTGTCGCTG
GGCGGCGTTTCGATGGGTATCGCCGGTTCCATTGTTGATCACAACTTCTTTGAATCCTGG
CTGGGAATGAAAGTCCAGGCGGTGGATATGACCGAACTGCGTCGCCGTATCGATCAGAAG
ATTTACGACGAAGCCGAATTGGAAATGGCACTGGCCTGGGCTGATAAAAACTTCCGCTAT
GGCGAAGATGAAAATAACAAACAGTATCAACGTAATGCCGAGCAAAGCCGCGCAGTTCTG
CGCGAAAGTTTACTGATGGCGATGTGTATCCGCGACATGATGCAAGGCAACAGCAAACTG
GCCGATATTGGTCGCGTGGAAGAATCACTTGGCTACAACGCCATCGCTGCGGGCTTCCAG
GGGCAACGTCACTGGACCGATCAATATCCCAATGGTGACACCGCCGAAGCGATCCTCAAC
AGTTCATTTGACTGGAATGGCGTGCGCGAACCCTTTGTCGTGGCGACCGAAAACGACAGT
CTTAACGGCGTGGCAATGCTAATGGGTCACCAGCTCACCGGCACCGCTCAGGTATTTGCC
GATGTGCGTACCTACTGGTCACCAGAAGCAATTGAGCGTGTAACGGGGCATAAACTGGAT
GGACTGGCAGAACACGGCATCATCCATTTGATCAACTCCGGTTCTGCTGCGCTGGACGGT
TCCTGTAAACAACGCGACAGCGAAGGTAACCCGACGATGAAGCCACACTGGGAAATCTCT
CAGCAAGAGGCTGACGCTTGCCTCGCCGCTACCGAATGGTGCCCGGCGATCCACGAATAC
TTCCGTGGCGGCGGTTACTCTTCCCGCTTCCTTACCGAAGGCGGCGTCCCGTTCACCATG
ACTCGTGTCAACATCATCAAAGGCCTGGGACCGGTACTGCAAATCGCGGAAGGCTGGAGC
GTGGAATTGCCGAAGGATGTGCATGACATCCTCAACAAACGCACCAACTCAACCTGGCCA
ACCACCTGGTTTGCACCGCGCCTCACCGGTAAAGGGCCGTTTACGGATGTGTACTCGGTA
ATGGCGAACTGGGGCGCTAACCATGGGGTTCTGACCATCGGCCACGTTGGCGCAGACTTT
ATCACTCTCGCCTCCATGCTGCGTATCCCGGTATGTATGCACAACGTTGAAGAGACCAAA
GTGTATCGTCCTTCTGCCTGGGCTGCGCACGGCATGGATATTGAAGGCCAGGATTACCGC
GCTTGCCAGAACTACGGTCCGTTGTACAAGCGTTAA
Protein Properties
Pfam Domain Function:
Protein Residues:591
Protein Molecular Weight:64976
Protein Theoretical pI:6
PDB File:1FUI
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>L-fucose isomerase
MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEKLRHACGAAVECVISDT
CIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPTRPKAIWGFNGTERPGAVY
LAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLLRFARAGLAVASMKGKSYLSL
GGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRIDQKIYDEAELEMALAWADKNFRY
GEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQGNSKLADIGRVEESLGYNAIAAGFQ
GQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVVATENDSLNGVAMLMGHQLTGTAQVFA
DVRTYWSPEAIERVTGHKLDGLAEHGIIHLINSGSAALDGSCKQRDSEGNPTMKPHWEIS
QQEADACLAATEWCPAIHEYFRGGGYSSRFLTEGGVPFTMTRVNIIKGLGPVLQIAEGWS
VELPKDVHDILNKRTNSTWPTTWFAPRLTGKGPFTDVYSVMANWGANHGVLTIGHVGADF
ITLASMLRIPVCMHNVEETKVYRPSAWAAHGMDIEGQDYRACQNYGPLYKR
References
External Links:
ResourceLink
Uniprot ID:P69922
Uniprot Name:FUCI_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85675621
PDB ID:1FUI
Ecogene ID:EG10349
Ecocyc:EG10349
ColiBase:b2802
Kegg Gene:b2802
EchoBASE ID:EB0345
CCDB:FUCI_ECOLI
BacMap:16130709
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • GREEN, M., COHEN, S. S. (1956). "Enzymatic conversion of L-fucose to L-fuculose." J Biol Chem 219:557-568. Pubmed: 13319278
  • Gunn, F. J., Tate, C. G., Henderson, P. J. (1994). "Identification of a novel sugar-H+ symport protein, FucP, for transport of L-fucose into Escherichia coli." Mol Microbiol 12:799-809. Pubmed: 8052131
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Lu, Z., Lin, E. C. (1989). "The nucleotide sequence of Escherichia coli genes for L-fucose dissimilation." Nucleic Acids Res 17:4883-4884. Pubmed: 2664711
  • Seemann, J. E., Schulz, G. E. (1997). "Structure and mechanism of L-fucose isomerase from Escherichia coli." J Mol Biol 273:256-268. Pubmed: 9367760