Spermidine export protein mdtJ (P69212)

Identification
Name:Spermidine export protein mdtJ
Synonyms:Not Available
Gene Name:mdtJ
Enzyme Class:Not Available
Biological Properties
General Function:Involved in spermidine transmembrane transporter activity
Specific Function:Catalyzes the excretion of spermidine. Can also confer resistance to deoxycholate and SDS
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Transports:
Transport References:
  • Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
Metabolites:
ECMDB IDNameView
ECMDB21225Hydrogen ionMetaboCard
ECMDB01257SpermidineMetaboCard
GO Classification:
Component
cell part
integral to membrane
intrinsic to membrane
membrane part
Gene Properties
Blattner:b1600
Gene OrientationCounterclockwise
Centisome Percentage:36.02
Left Sequence End1671160
Right Sequence End1671525
Gene Sequence:
>366 bp
ATGATCAGCAGAGTGACAGAAGCTCTAAGCAAAGTTAAAGGATCGATGGGAAGCCACGAG
CGCCATGCATTGCCTGGTGTTATCGGTGACGATCTTTTGCGATTTGGGAAGCTGCCACTC
TGCCTGTTCATTTGCATTATTTTGACGGCGGTGACTGTGGTAACCACGGCGCACCATACC
CGTTTACTGACCGCTCAGCGCGAACAACTGGTGCTGGAGCGAGATGCTTTAGACATTGAA
TGGCGCAACCTGATCCTTGAAGAGAATGCGCTCGGCGACCATAGCCGGGTGGAAAGGATC
GCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGAAAATATCGTAGTGCAA
AAATAA
Protein Properties
Pfam Domain Function:
Protein Residues:121
Protein Molecular Weight:13115
Protein Theoretical pI:9
Signaling Regions:
  • None
Transmembrane Regions:
  • 2-22
  • 32-52
  • 55-75
  • 82-102
Protein Sequence:
>Spermidine export protein mdtJ
MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLSYIFLSFAVKKIALGVA
YALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLIKSGTRKARKPELEVNHGA
V
References
External Links:
ResourceLink
Uniprot ID:P69212
Uniprot Name:MDTJ_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674321
Ecogene ID:EG13927
Ecocyc:EG13927
ColiBase:b1600
Kegg Gene:b1600
EchoBASE ID:EB3686
CCDB:MDTJ_ECOLI
BacMap:16129558
General Reference:
  • Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kasai, H., Kashimoto, K., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Horiuchi, T., et, a. l. .. (1996). "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." DNA Res 3:363-377. Pubmed: 9097039
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Higashi, K., Ishigure, H., Demizu, R., Uemura, T., Nishino, K., Yamaguchi, A., Kashiwagi, K., Igarashi, K. (2008). "Identification of a spermidine excretion protein complex (MdtJI) in Escherichia coli." J Bacteriol 190:872-878. Pubmed: 18039771
  • Nishino, K., Yamaguchi, A. (2001). "Analysis of a complete library of putative drug transporter genes in Escherichia coli." J Bacteriol 183:5803-5812. Pubmed: 11566977