Identification
Name:Low affinity potassium transport system protein kup
Synonyms:
  • Kup system potassium uptake protein
Gene Name:kup
Enzyme Class:Not Available
Biological Properties
General Function:Involved in potassium ion transmembrane transporter activity
Specific Function:Responsible for the low-affinity transport of potassium into the cell, with the probable concomitant uptake of protons (symport system). Can also transport cesium
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Transports:
Transport References:
  • Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
Metabolites:
ECMDB IDNameView
ECMDB21225Hydrogen ionMetaboCard
ECMDB04139PotassiumMetaboCard
GO Classification:
Component
cell part
membrane
Function
cation transmembrane transporter activity
inorganic cation transmembrane transporter activity
ion transmembrane transporter activity
monovalent inorganic cation transmembrane transporter activity
potassium ion transmembrane transporter activity
substrate-specific transmembrane transporter activity
transmembrane transporter activity
transporter activity
Process
cation transport
establishment of localization
ion transport
monovalent inorganic cation transport
potassium ion transport
transport
Gene Properties
Blattner:b3747
Gene OrientationClockwise
Centisome Percentage:84.69
Left Sequence End3929339
Right Sequence End3931207
Gene Sequence:
>1869 bp
ATGAGCACTGATAATAAGCAATCATTGCCCGCGATTACCCTCGCGGCGATTGGAGTTGTC
TACGGCGATATTGGTACCAGCCCGTTATATACACTTCGTGAATGTTTGTCCGGCCAGTTT
GGTTTTGGCGTTGAACGCGATGCCGTGTTTGGCTTTTTATCGCTGATCTTCTGGCTGCTA
ATCTTTGTGGTTTCCATTAAATATCTCACCTTCGTGATGCGGGCAGATAACGCCGGTGAG
GGGGGGATCCTGACGTTGATGTCGCTTGCCGGGCGTAATACGTCGGCGCGAACCACATCA
ATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACA
CCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGAT
ACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACAT
GGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTG
GCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCA
ATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCA
GTGGTGTTGTCGATTACGGGGGTCGAGGCGCTGTATGCTGATATGGGGCACTTTGGTAAG
TTCCCTATTCGCCTGGCGTGGTTCACCGTCGTATTACCTTCCTTGACCCTTAATTACTTC
GGTCAGGGAGCGCTGTTGTTAAAGAACCCGGAAGCGATTAAGAACCCGTTCTTCCTGTTG
GCACCGGACTGGGCGCTGATCCCGCTGCTGATCATCGCCGCACTGGCGACGGTAATTGCC
TCGCAGGCGGTTATCTCTGGCGTCTTCTCATTGACGCGTCAGGCGGTACGTCTGGGATAT
TTGTCGCCGATGCGCATTATTCACACCTCCGAAATGGAGTCAGGGCAAATCTATATTCCG
TTTGTGAACTGGATGCTCTATGTCGCGGTCGTGATTGTGATTGTCAGCTTTGAGCACTCC
AGCAACCTGGCGGCGGCGTACGGGATTGCGGTGACCGGAACCATGGTGCTGACGTCTATT
CTCTCGACTACCGTGGCACGTCAGAACTGGCACTGGAATAAGTATTTTGTTGCGCTGATC
CTGATTGCTTTCCTTTGTGTCGATATTCCATTGTTCACCGCTAACCTCGATAAACTGCTC
TCCGGCGGCTGGTTGCCATTGAGCCTCGGTACTGTGATGTTTATCGTGATGACCACCTGG
AAGAGCGAGCGTTTCCGCTTGCTGCGGCGGATGCATGAACATGGTAACTCTCTGGAAGCG
ATGATTGCTTCGCTGGAGAAATCACCGCCTGTTCGCGTGCCCGGGACCGCGGTGTATATG
TCGCGTGCAATCAACGTCATTCCCTTTGCGCTGATGCATAACCTTAAACATAACAAGGTA
TTGCATGAGCGGGTGATTCTGTTAACTCTGCGCACCGAAGACGCTCCATATGTCCATAAC
GTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTAT
GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTA
AGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCATGAGTCGTTGATCCTCGGCAAA
CGCCCGTGGTATTTGCGTCTGCGCGGCAAGCTGTACTTGCTGCTGCAACGTAATGCGCTG
CGTGCACCAGATCAATTTGAAATCCCGCCAAACAGGGTTATCGAACTGGGTACTCAGGTC
GAAATCTAA
Protein Properties
Pfam Domain Function:
Protein Residues:622
Protein Molecular Weight:69293
Protein Theoretical pI:9
Signaling Regions:
  • None
Transmembrane Regions:
  • 9-29
  • 49-69
  • 103-123
  • 137-157
  • 165-185
  • 213-233
  • 247-267
  • 276-296
  • 337-357
  • 363-383
  • 396-416
  • 419-439
Protein Sequence:
>Low affinity potassium transport system protein kup
MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGVERDAVFGFLSLIFWLL
IFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSMLVIMGLIGGSFFYGEVVIT
PAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKHGTAMVGKLFAPIMLTWFLIL
AGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIALGAVVLSITGVEALYADMGHFGK
FPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNPFFLLAPDWALIPLLIIAALATVIA
SQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMESGQIYIPFVNWMLYVAVVIVIVSFEHS
SNLAAAYGIAVTGTMVLTSILSTTVARQNWHWNKYFVALILIAFLCVDIPLFTANLDKLL
SGGWLPLSLGTVMFIVMTTWKSERFRLLRRMHEHGNSLEAMIASLEKSPPVRVPGTAVYM
SRAINVIPFALMHNLKHNKVLHERVILLTLRTEDAPYVHNVRRVQIEQLSPTFWRVVASY
GWRETPNVEEVFHRCGLEGLSCRMMETSFFMSHESLILGKRPWYLRLRGKLYLLLQRNAL
RAPDQFEIPPNRVIELGTQVEI
References
External Links:
ResourceLink
Uniprot ID:P63183
Uniprot Name:KUP_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85676291
Ecogene ID:EG11541
Ecocyc:EG11541
ColiBase:b3747
Kegg Gene:b3747
EchoBASE ID:EB1503
CCDB:KUP_ECOLI
BacMap:49176400
General Reference:
  • Blattner, F. R., Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L. (1993). "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Nucleic Acids Res 21:5408-5417. Pubmed: 8265357
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Bossemeyer, D., Schlosser, A., Bakker, E. P. (1989). "Specific cesium transport via the Escherichia coli Kup (TrkD) K+ uptake system." J Bacteriol 171:2219-2221. Pubmed: 2649491
  • Burland, V., Plunkett, G. 3rd, Daniels, D. L., Blattner, F. R. (1993). "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication." Genomics 16:551-561. Pubmed: 7686882
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Riley, M., Abe, T., Arnaud, M. B., Berlyn, M. K., Blattner, F. R., Chaudhuri, R. R., Glasner, J. D., Horiuchi, T., Keseler, I. M., Kosuge, T., Mori, H., Perna, N. T., Plunkett, G. 3rd, Rudd, K. E., Serres, M. H., Thomas, G. H., Thomson, N. R., Wishart, D., Wanner, B. L. (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34:1-9. Pubmed: 16397293
  • Schleyer, M., Bakker, E. P. (1993). "Nucleotide sequence and 3'-end deletion studies indicate that the K(+)-uptake protein kup from Escherichia coli is composed of a hydrophobic core linked to a large and partially essential hydrophilic C terminus." J Bacteriol 175:6925-6931. Pubmed: 8226635
  • Trchounian, A., Kobayashi, H. (1999). "Kup is the major K+ uptake system in Escherichia coli upon hyper-osmotic stress at a low pH." FEBS Lett 447:144-148. Pubmed: 10214935
  • Zakharyan, E., Trchounian, A. (2001). "K+ influx by Kup in Escherichia coli is accompanied by a decrease in H+ efflux." FEMS Microbiol Lett 204:61-64. Pubmed: 11682179