ECMDB

Identification

Name:

Carbonic anhydrase 2

Synonyms:

Carbonate dehydratase 2

Gene Name:

can

Enzyme Class:

4.2.1.1

Biological Properties

General Function:

Involved in carbonate dehydratase activity

Specific Function:

H(2)CO(3) = CO(2) + H(2)O

Cellular Location:

Not Available

SMPDB Pathways:

Nitrogen metabolism PW000755
cyanate degradation PW002099

KEGG Pathways:

Nitrogen metabolism ec00910

KEGG Reactions:

1.0

↔

1.0

1.0Carbonic acid ↔ 1.0Carbon dioxide + 1.0Water
ReactionCard

SMPDB Reactions:

1.0

→

1.0

1.0Carbon dioxide + 1.0Water → 1.0Hydrogen carbonate
ReactionCard

1.0

→

1.0

1.0Carbon dioxide + 1.0Water → 1.0Hydrogen ion + 1.0Hydrogen carbonate
ReactionCard

EcoCyc Reactions:

1.0

↔

1.0

1.0Carbon dioxide + 1.0Water ↔ 1.0Hydrogen ion + 1.0Hydrogen carbonate
ReactionCard

Complex Reactions:

1.0

→

1.0

1.0Carbonic acid → 1.0Carbon dioxide + 1.0Water
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB04030	Carbon dioxide	MetaboCard
ECMDB03538	Carbonic acid	MetaboCard
ECMDB00595	Hydrogen carbonate	MetaboCard
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB00494	Water	MetaboCard

GO Classification:

Function
binding
carbon-oxygen lyase activity
carbonate dehydratase activity
catalytic activity
cation binding
hydro-lyase activity
ion binding
lyase activity
metal ion binding
transition metal ion binding
zinc ion binding
Process
carbon utilization

Gene Properties

Blattner:

b0126

Gene Orientation

Counterclockwise

Centisome Percentage:

3.06

Left Sequence End

142008

Right Sequence End

142670

Gene Sequence:

>663 bp
ATGAAAGACATAGATACACTCATCAGCAACAATGCACTATGGTCAAAAATGCTGGTGGAA
GAGGATCCCGGGTTTTTTGAGAAACTGGCACAAGCGCAAAAACCGCGCTTTCTATGGATT
GGATGTTCCGACAGTCGCGTTCCTGCAGAACGTTTAACCGGTCTTGAGCCGGGCGAACTC
TTTGTTCACCGTAATGTTGCTAACCTGGTCATTCACACTGACCTGAACTGCCTTTCCGTG
GTTCAGTATGCAGTGGATGTACTCGAAGTTGAACACATTATTATCTGTGGCCACTACGGT
TGCGGCGGCGTACAAGCCGCAGTTGAAAACCCGGAACTGGGGCTTATCAACAACTGGCTG
CTGCATATCCGCGATATCTGGTTCAAACATAGCTCATTGCTCGGCGAAATGCCGCAAGAG
CGCCGTCTGGATACCTTGTGTGAACTGAACGTCATGGAACAGGTGTATAACCTGGGCCAC
TCCACCATTATGCAATCAGCGTGGAAACGCGGGCAGAAAGTTACCATTCACGGCTGGGCC
TACGGCATTCACGACGGCTTGCTGCGTGATCTGGATGTTACCGCCACCAACCGCGAAACC
CTTGAGCAACGTTACCGTCACGGGATTTCCAACCTCAAGCTGAAACACGCCAACCACAAA
TAA

Protein Properties

Pfam Domain Function:

Pro_CA (PF00484 )

Protein Residues:

220

Protein Molecular Weight:

25097

Protein Theoretical pI:

PDB File:

1T75

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Carbonic anhydrase 2
MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSDSRVPAERLTGLEPGEL
FVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCGGVQAAVENPELGLINNWL
LHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGHSTIMQSAWKRGQKVTIHGWA
YGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHANHK

References

External Links:

Resource	Link
Uniprot ID:	P61517
Uniprot Name:	CAN_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85674342
PDB ID:	1T75
Ecogene ID:	EG12319
Ecocyc:	EG12319
ColiBase:	b0126
Kegg Gene:	b0126
EchoBASE ID:	EB2224
CCDB:	CAN_ECOLI
BacMap:	16128119

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Cronk, J. D., Endrizzi, J. A., Cronk, M. R., O'neill, J. W., Zhang, K. Y. (2001). "Crystal structure of E. coli beta-carbonic anhydrase, an enzyme with an unusual pH-dependent activity." Protein Sci 10:911-922. Pubmed: 11316870
Fountoulakis, M., Takacs, M. F., Berndt, P., Langen, H., Takacs, B. (1999). "Enrichment of low abundance proteins of Escherichia coli by hydroxyapatite chromatography." Electrophoresis 20:2181-2195. Pubmed: 10493123
Fujita, N., Mori, H., Yura, T., Ishihama, A. (1994). "Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region." Nucleic Acids Res 22:1637-1639. Pubmed: 8202364
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553

Carbonic anhydrase 2 (P61517)