GTP-binding protein lepA (P60785)

Identification
Name:GTP-binding protein lepA
Synonyms:Not Available
Gene Name:lepA
Enzyme Class:Not Available
Biological Properties
General Function:Cell wall/membrane/envelope biogenesis
Specific Function:Not Available
Cellular Location:Cell membrane. Periplasm
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Guanosine triphosphate + 1.0Water → 1.0Guanosine diphosphate + 1.0Inorganic phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB01201Guanosine diphosphateMetaboCard
ECMDB01273Guanosine triphosphateMetaboCard
ECMDB21380Inorganic phosphateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
binding
catalytic activity
GTP binding
GTPase activity
guanyl nucleotide binding
guanyl ribonucleotide binding
hydrolase activity
hydrolase activity, acting on acid anhydrides
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
nucleoside-triphosphatase activity
nucleotide binding
purine nucleotide binding
pyrophosphatase activity
Gene Properties
Blattner:Not Available
Gene OrientationNot Available
Centisome Percentage:Not Available
Left Sequence EndNot Available
Right Sequence EndNot Available
Gene Sequence:
>1800 bp
ATGAAGAATATACGTAACTTTTCGATCATAGCTCACATTGACCACGGTAAATCGACGCTG
TCTGACCGTATTATCCAGATCTGCGGTGGCCTGTCTGACCGTGAAATGGAGGCGCAGGTT
CTCGATTCCATGGATCTTGAGCGTGAGCGTGGCATTACCATCAAAGCGCAAAGCGTGACG
CTGGACTACAAAGCGTCTGACGGCGAAACCTATCAGCTTAACTTTATCGACACCCCGGGC
CACGTAGACTTCTCCTATGAAGTTTCCCGTTCGCTGGCTGCCTGTGAAGGTGCATTGCTG
GTGGTCGACGCCGGGCAGGGCGTAGAAGCGCAAACCCTGGCAAACTGCTACACCGCCATG
GAAATGGATCTCGAAGTTGTGCCGGTACTGAACAAGATTGACCTGCCGGCAGCCGATCCT
GAACGCGTGGCGGAAGAAATTGAAGATATCGTCGGCATCGACGCCACCGACGCGGTGCGC
TGTTCAGCGAAAACCGGCGTTGGTGTGCAGGACGTTCTCGAACGTCTGGTGCGCGACATT
CCGCCGCCGGAAGGCGATCCGGAAGGCCCGTTGCAGGCACTAATTATCGACTCATGGTTC
GACAACTACCTGGGCGTTGTTTCACTTATCCGTATTAAAAACGGCACCCTGCGTAAGGGC
GACAAAGTGAAAGTCATGAGTACCGGGCAGACCTATAACGCCGACCGTCTGGGCATCTTC
ACGCCGAAACAGGTTGACCGCACTGAACTGAAATGTGGCGAAGTAGGCTGGCTCGTATGT
GCGATTAAAGATATCCACGGCGCTCCAGTCGGCGATACCTTAACGCTGGCGCGTAATCCG
GCAGAAAAGGCGCTGCCTGGCTTTAAGAAAGTCAAACCGCAGGTATACGCCGGTCTGTTC
CCGGTAAGTTCCGACGACTATGAAGCCTTCCGTGACGCGCTGGGTAAACTCAGCCTGAAC
GATGCCTCACTGTTCTATGAGCCGGAAAGCTCCAGCGCGCTGGGCTTTGGTTTCCGCTGC
GGCTTCCTCGGCCTGCTGCACATGGAGATCATCCAGGAACGTCTGGAACGTGAATACGAT
CTGGATCTGATCACCACTGCGCCGACCGTAGTGTATGAAGTTGAAACCACGTCAAGAGAA
GTTATCTACGTCGACAGCCCATCCAAGCTGCCTGCGGTAAATAACATCTACGAACTGCGC
GAGCCGATTGCAGAGTGTCACATGCTGCTGCCGCAGGCATATCTCGGCAACGTTATTACG
TTGTGCGTAGAAAAACGCGGCGTGCAGACCAATATGGTTTACCACGGTAATCAGGTGGCG
CTGACGTACGAGATCCCGATGGCGGAAGTGGTGCTCGACTTCTTCGATCGCCTGAAATCT
ACCTCGCGTGGTTATGCGTCTCTGGATTACAACTTCAAGCGCTTCCAGGCGTCCGACATG
GTACGTGTAGACGTATTAATCAACGGTGAACGTGTTGATGCGCTGGCGTTGATCACCCAC
CGTGATAATTCGCAAAACCGCGGTCGCGAGTTGGTGGAGAAGATGAAAGATCTGATCCCA
CGCCAGCAGTTTGATATCGCCATTCAGGCAGCGATTGGTACGCACATCATTGCGCGATCC
ACCGTGAAACAGCTGCGTAAAAACGTACTGGCTAAATGTTATGGCGGCGATATCAGCCGT
AAGAAAAAGCTGCTGCAGAAGCAGAAAGAAGGTAAGAAACGCATGAAGCAGATCGGTAAC
GTCGAGCTGCCGCAGGAAGCGTTCCTCGCCATTCTGCACGTCGGCAAAGACAACAAATAA
Protein Properties
Pfam Domain Function:
Protein Residues:599
Protein Molecular Weight:66570
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>GTP-binding protein lepA
MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSMDLERERGITIKAQSVT
LDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAGQGVEAQTLANCYTAM
EMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVRCSAKTGVGVQDVLERLVRDI
PPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLRKGDKVKVMSTGQTYNADRLGIF
TPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTLARNPAEKALPGFKKVKPQVYAGLF
PVSSDDYEAFRDALGKLSLNDASLFYEPESSSALGFGFRCGFLGLLHMEIIQERLEREYD
LDLITTAPTVVYEVETTSREVIYVDSPSKLPAVNNIYELREPIAECHMLLPQAYLGNVIT
LCVEKRGVQTNMVYHGNQVALTYEIPMAEVVLDFFDRLKSTSRGYASLDYNFKRFQASDM
VRVDVLINGERVDALALITHRDNSQNRGRELVEKMKDLIPRQQFDIAIQAAIGTHIIARS
TVKQLRKNVLAKCYGGDISRKKKLLQKQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK
References
External Links:
ResourceLink
Uniprot ID:P60785
Uniprot Name:LEPA_ECOLI
GenBank Gene ID:U00096
Genebank Protein ID:1788922
CCDB:LEPA_ECOLI
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Kushiro, M., Shimizu, M., Tomita, K. (1987). "Molecular cloning and sequence determination of the tuf gene coding for the elongation factor Tu of Thermus thermophilus HB8." Eur J Biochem 170:93-98. Pubmed: 2826164
  • March, P. E., Inouye, M. (1985). "Characterization of the lep operon of Escherichia coli. Identification of the promoter and the gene upstream of the signal peptidase I gene." J Biol Chem 260:7206-7213. Pubmed: 2987248
  • March, P. E., Inouye, M. (1985). "GTP-binding membrane protein of Escherichia coli with sequence homology to initiation factor 2 and elongation factors Tu and G." Proc Natl Acad Sci U S A 82:7500-7504. Pubmed: 2999765