4-alpha-L-fucosyltransferase (P56258)

Identification
Name:4-alpha-L-fucosyltransferase
Synonyms:
  • TDP-Fuc4NAc:lipid II Fuc4NAc transferase
  • Fuc4NAc transferase
Gene Name:wecF
Enzyme Class:Not Available
Biological Properties
General Function:Involved in fucosyltransferase activity
Specific Function:Catalyzes the synthesis of Und-PP-GlcNAc-ManNAcA-Fuc4NAc (Lipid III), the third lipid-linked intermediate involved in ECA synthesis
Cellular Location:Cell inner membrane; Peripheral membrane protein (Potential)
SMPDB Pathways:
  • Secondary Metabolites: enterobacterial common antigen biosynthesis PW000959
  • Secondary Metabolites: enterobacterial common antigen biosynthesis 2 PW002045
  • Secondary Metabolites: enterobacterial common antigen biosynthesis 3 PW002046
KEGG Pathways:Not Available
SMPDB Reactions:
1.0Undecaprenyl N-acetyl-glucosaminyl-N-acetyl-mannosaminuronate pyrophosphate+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Undecaprenyl N-acetyl-glucosaminyl-N-acetyl-mannosaminuronate-4-acetamido-4,6-dideoxy-D-galactose pyrophosphate+1.0Thumb
1.0Undecaprenyl N-acetyl-glucosaminyl-N-acetyl-mannosaminuronate pyrophosphate + 1.0TDP-Fuc4NAc + 1.0Undecaprenyl-diphospho-N-acetylglucosamine-N-acetylmannosaminuronate → 1.0Hydrogen ion + 1.0dTDP + 1.0Undecaprenyl N-acetyl-glucosaminyl-N-acetyl-mannosaminuronate-4-acetamido-4,6-dideoxy-D-galactose pyrophosphate + 1.0Undecaprenyl-diphospho N-acetylglucosamine-N-acetylmannosaminuronate-N-acetamido-4,6-dideoxy-D-galactose
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0dTDP-4-Acetamido-4,6-dideoxy-D-galactose + 1.0Undecaprenyl-diphospho-N-acetylglucosamine-N-acetylmannosaminuronate → 1.0dTDP + 1.0Hydrogen ion + 1.0Undecaprenyl-diphospho N-acetylglucosamine-N-acetylmannosaminuronate-N-acetamido-4,6-dideoxy-D-galactose
ReactionCard
1.0Thumb+1.0Und-PP-GlcNAc-ManNAcA → 1.0Thumb+1.0Und-PP-GlcNAc-ManNAcA-Fuc4NAc
1.0TDP-Fuc4NAc + 1.0Und-PP-GlcNAc-ManNAcA → 1.0Thiamine pyrophosphate + 1.0Und-PP-GlcNAc-ManNAcA-Fuc4NAc
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB01274dTDPMetaboCard
ECMDB23903dTDP-4-acetamido-4,6-dideoxy-alpha-D-galactoseMetaboCard
ECMDB20613dTDP-4-Acetamido-4,6-dideoxy-D-galactoseMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB23181TDP-Fuc4NAcMetaboCard
ECMDB01372Thiamine pyrophosphateMetaboCard
ECMDB20208Undecaprenyl phosphateMetaboCard
ECMDB21297Undecaprenyl-diphospho N-acetylglucosamine-N-acetylmannosaminuronate-N-acetamido-4,6-dideoxy-D-galactoseMetaboCard
ECMDB21298Undecaprenyl-diphospho-N-acetylglucosamine-N-acetylmannosaminuronateMetaboCard
GO Classification:
Component
cell part
cell wall
external encapsulating structure
Gram-negative-bacterium-type cell wall
peptidoglycan-based cell wall
Function
catalytic activity
fucosyltransferase activity
transferase activity
transferase activity, transferring glycosyl groups
transferase activity, transferring hexosyl groups
Process
carbohydrate metabolic process
cellular polysaccharide biosynthetic process
enterobacterial common antigen biosynthetic process
metabolic process
polysaccharide biosynthetic process
polysaccharide metabolic process
primary metabolic process
Gene Properties
Blattner:b4481
Gene OrientationClockwise
Centisome Percentage:85.69
Left Sequence End3975548
Right Sequence End3976627
Gene Sequence:
>1080 bp
ATGACTGTACTGATTCACGTACTGGGATCGGATATCCCTCACCATAACCGAACCGTTTTG
CGGTTTTTCAATGACGCGCTGGCCGCGACGAGCGAGCACGCGCGCGAGTTTATGGTTGTT
GGCAAGGACGACGGCTTAAGTGATAGCTGTCCGGCGCTTTCTGTGCAATTTTTCCCTGGG
AAAAAATCGCTGGCGGAAGCGGTCATCGCGAAAGCAAAAGCTAACCGTCAGCAGCGTTTT
TTCTTCCACGGTCAGTTCAATCCCACACTGTGGCTGGCTCTGCTGAGTGGTGGCATTAAG
CCCAGCCAGTTTTTCTGGCATATCTGGGGGGCAGACCTGTATGAGCTTTCCAGTGGCTTG
AGATATAAGCTTTTTTACCCACTACGTCGCCTGGCGCAAAAGCGAGTCGGCTGTGTATTT
GCCACCCGCGGCGATTTGAGCTTTTTTGCCAAAACGCACCCAAAGGTGCGGGGCGAACTG
CTGTTCTTCCCGACGCGGATGGACCCTTCGCTCAATACGATGGCGAACGATCGGCAACGT
GAAGGGAAAATGACCATTCTGGTGGGGAACTCCGGCGACCGCAGCAATGAGCATATTGCT
GCCTTGCGCGCCGTTCATCAGCAATTTGGCGATACGGTAAAAGTGGTGGTGCCGATGGGA
TATCCGCCTAATAACGAAGCGTACATTGAGGAAGTTCGTCAGGCGGGGCTGGAGTTATTC
AGCGAAGAAAATCTACAAATTCTGAGCGAAAAACTGGAATTTGACGCCTATCTGGCGCTA
CTTCGTCAGTGCGATCTTGGTTACTTTATTTTTGCCCGCCAGCAGGGCATTGGTACGCTG
TGCTTACTGATTCAGGCGGGCATTCCTTGTGTGCTTAACCGGGAAAATCCGTTCTGGCAG
GATATGACGGAACAACATTTGCCGGTGCTGTTTACTACCGACGATCTCAACGAGGATATT
GTGCGTGAAGCGCAGCGCCAGTTGGCGTCGGTGGATAAAAACACCATTGCCTTCTTTAGC
CCTAACTATCTACAAGGCTGGCAGCGGGCGTTGGCGATTGCCGCCAGGGAGGTCGCATGA
Protein Properties
Pfam Domain Function:
Protein Residues:359
Protein Molecular Weight:40639
Protein Theoretical pI:8
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>4-alpha-L-fucosyltransferase
MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDDGLSDSCPALSVQFFPG
KKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPSQFFWHIWGADLYELSSGL
RYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGELLFFPTRMDPSLNTMANDRQR
EGKMTILVGNSGDRSNEHIAALRAVHQQFGDTVKVVVPMGYPPNNEAYIEEVRQAGLELF
SEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQGIGTLCLLIQAGIPCVLNRENPFWQ
DMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKNTIAFFSPNYLQGWQRALAIAAREVA
References
External Links:
ResourceLink
Uniprot ID:P56258
Uniprot Name:WECF_ECOLI
GenBank Gene ID:AF375882
Genebank Protein ID:14485183
Ecogene ID:EG14320
Ecocyc:EG14320
ColiBase:b4481
Kegg Gene:b4481
EchoBASE ID:EB4066
CCDB:WECF_ECOLI
BacMap:49176413
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Daniels, D. L., Plunkett, G. 3rd, Burland, V., Blattner, F. R. (1992). "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes." Science 257:771-778. Pubmed: 1379743
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Rahman, A., Barr, K., Rick, P. D. (2001). "Identification of the structural gene for the TDP-Fuc4NAc:lipid II Fuc4NAc transferase involved in synthesis of enterobacterial common antigen in Escherichia coli K-12." J Bacteriol 183:6509-6516. Pubmed: 11673418
  • Riley, M., Abe, T., Arnaud, M. B., Berlyn, M. K., Blattner, F. R., Chaudhuri, R. R., Glasner, J. D., Horiuchi, T., Keseler, I. M., Kosuge, T., Mori, H., Perna, N. T., Plunkett, G. 3rd, Rudd, K. E., Serres, M. H., Thomas, G. H., Thomson, N. R., Wishart, D., Wanner, B. L. (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34:1-9. Pubmed: 16397293