Alpha-mannosidase mngB (P54746)

Identification
Name:Alpha-mannosidase mngB
Synonyms:Not Available
Gene Name:mngB
Enzyme Class:
Biological Properties
General Function:Involved in catalytic activity
Specific Function:May hydrolyze mannosyl-D-glycerate to mannose-6- phosphate and glycerate
Cellular Location:Cytoplasmic
SMPDB Pathways:
  • 2-O-alpha-mannosyl-D-glycerate degradation PW002096
KEGG Pathways:Not Available
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.02(alpha-D-Mannosyl-6-phosphate)-D-glycerate + 1.0Water ↔ 1.0Mannose 6-phosphate + 1.0Glyceric acid
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb → 1.0Mannose 6-phosphate+1.0Thumb
1.02-O-(6-Phospho-alpha-D-mannosyl)-D-glycerate + 1.0Water → 1.0Mannose 6-phosphate + 1.0Glyceric acid
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Water + 1.02(alpha-D-Mannosyl-6-phosphate)-D-glycerate → 1.0Glyceric acid + 1.0Mannose 6-phosphate
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.02-O-(6-Phospho-alpha-D-mannosyl)-D-glycerate + 1.0Water → 1.0Mannose 6-phosphate + 1.0Glyceric acid
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB211352(alpha-D-Mannosyl-6-phosphate)-D-glycerateMetaboCard
ECMDB230112-O-(6-Phospho-alpha-D-mannosyl)-D-glycerateMetaboCard
ECMDB04077Glyceric acidMetaboCard
ECMDB01078Mannose 6-phosphateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
alpha-mannosidase activity
binding
catalytic activity
cation binding
hydrolase activity
hydrolase activity, acting on glycosyl bonds
hydrolase activity, hydrolyzing O-glycosyl compounds
ion binding
mannosidase activity
metal ion binding
transition metal ion binding
zinc ion binding
Process
alcohol metabolic process
carbohydrate metabolic process
hexose metabolic process
mannose metabolic process
metabolic process
monosaccharide metabolic process
primary metabolic process
small molecule metabolic process
Gene Properties
Blattner:b0732
Gene OrientationClockwise
Centisome Percentage:16.54
Left Sequence End767201
Right Sequence End769834
Gene Sequence:
>2634 bp
ATGAAAGCAGTATCTCGCGTTCACATCACCCCGCATATGCACTGGGATCGAGAGTGGTAT
TTCACCACCGAAGAGTCACGTATTCTGCTGGTCAATAATATGGAAGAGATCCTGTGCCGA
CTGGAACAGGACAACGAATACAAATATTACGTACTCGACGGGCAAACGGCGATCCTCGAA
GATTATTTCGCGGTGAAACCGGAAAACAAAGACCGTGTGAAGAAACAGGTAGAAGCCGGC
AAGTTGATTATCGGCCCCTGGTATACCCAGACCGATACCACGATTGTTTCTGCGGAATCC
ATCGTCCGTAATCTGATGTACGGAATGCGTGACTGCCTCGCGTTTGGCGAGCCGATGAAA
ATAGGTTATTTACCAGATTCCTTTGGCATGTCCGGGCAACTGCCGCATATCTACAATGGA
TTTGGCATTACCCGCACCATGTTCTGGCGCGGATGTTCGGAGCGCCACGGTACTGATAAA
ACCGAGTTTTTGTGGCAAAGCAGTGACGGTAGCGAAGTGACGGCGCAGGTGCTGCCGCTG
GGCTACGCCATCGGTAAGTACTTACCTGCCGACGAAAACGGATTACGTAAACGCCTCGAC
AGTTATTTTGACGTGCTGGAAAAAGCGTCTGTAACCAAAGAGATTTTGCTGCCGAATGGG
CATGACCAGATGCCATTGCAGCAAAATATCTTCGAAGTGATGGATAAGCTACGTGAGATC
TACCCTCAACGTAAGTTTGTGATGAGCCGCTTTGAAGAGGTATTTGAGAAGATCGAAGCG
CAGCGAGATAATCTGGCAACCCTGAAAGGGGAATTTATTGATGGCAAATATATGCGCGTG
CATCGCACCATCGGTTCTACGCGTATGGATATCAAAATTGCCCACGCGCGTATTGAAAAT
AAGATTGTTAATCTGCTGGAACCGCTGGCAACACTGGCCTGGACGTTGGGTTTTGAATAC
CACCACGGCTTGCTGGAGAAAATGTGGAAAGAGATCTTAAAAAATCATGCCCACGACAGT
ATCGGCTGCTGCTGTAGTGACAAAGTTCATCGCGAAATCGTCGCCCGCTTCGAACTGGCT
GAAGACATGGCGGATAATCTGATTCGTTTCTACATGCGCAAAATTGCCGACAACATGCCG
CAGAGCGACGCCGACAAACTCGTCCTGTTTAACCTGATGCCCTGGCCGCGTGAAGAAGTT
ATCAACACCACTGTGCGGCTGCGCGCCAGCCAGTTTAATTTGCGGGACGATCGCGGTCAG
CCTGTACCGTATTTTATTCGCCATGCCCGTGAGATCGATCCAGGCCTAATCGATCGGCAA
ATAGTTCATTACGGTAATTACGATCCCTTTATGGAGTTTGATATACAGATCAACCAGATT
GTCCCTTCTATGGGCTATCGCACGCTTTATATCGAAGCGAATCAGCCTGGCAACGTAATT
GCGGCAAAAAGTGACGCTGAAGGGATACTGGAAAATGCTTTCTGGCAAATTGCGCTCAAT
GAGGATGGTTCTCTGCAACTGGTAGATAAAGACAGCGGTGTGCGCTATGACCGGGTATTG
CAAATTGAAGAAAGCTCTGATGATGGTGATGAATATGACTATTCACCCGCAAAAGAAGAG
TGGGTAATTACCGCAGCGAACGCGAAACCGCAATGCGATATTATTCATGAAGCCTGGCAG
AGCAGGGCTGTTATCCGCTATGACATGGCAGTGCCGCTCAATTTGTCAGAACGCAGCGCC
CGGCAATCCACTGGCAGAGTAGGGGTGGTGTTGGTTGTCACTCTTAGTCATAACAGCAGG
CGTATTGATGTGGATATCAATCTTGATAACCAGGCTGACGATCATCGCCTTCGTGTCCTG
GTCCCTACACCTTTTAACACCGACAGTGTTCTGGCAGATACGCAGTTTGGTTCGCTAACG
CGCCCCGTGAACGACAGTGCAATGAACAACTGGCAGCAAGAAGGCTGGAAAGAAGCGCCG
GTTCCGGTATGGAATATGCTCAACTATGTTGCCTTACAGGAAGGGCGTAACGGCATGGCT
GTCTTTAGCGAAGGGTTACGTGAATTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCC
ATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATCTGCTTTTAAGGCCTGGG
CGGCCTTCGGGAATTAAAATGCCAGTCCCGGACTCACAACTACGTGGTCTGCTTTCTTGT
CGCCTAAGTTTATTGAGTTATACCGGTACGCCAACCGCCGCTGGTGTAGCTCAGCAGGCG
CGAGCATGGCTGACTCCAGTACAGTGTTACAACAAAATCCCATGGGATGTGATGAAGCTC
AACAAAGCCGGATTCAACGTGCCGGAAAGTTATAGTTTGTTGAAAATGCCCCCAGTGGGA
TGCCTGATAAGCGCACTTAAGAAAGCTGAAGACCGACAAGAAGTGATTTTACGGCTGTTT
AATCCGGCTGAATCAGCAACCTGTGATGCGACTGTTGCTTTCAGTCGCGAGGTGATTTCT
TGCTCAGAAACGATGATGGATGAACACATTACCACCGAGGAAAATCAAGGTTCAAATCTA
TCGGGGCCTTTTTTACCCGGCCAGTCACGGACGTTCAGTTACCGGCTTGCCTGA
Protein Properties
Pfam Domain Function:
Protein Residues:877
Protein Molecular Weight:100014
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Alpha-mannosidase mngB
MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQDNEYKYYVLDGQTAILE
DYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIVRNLMYGMRDCLAFGEPMK
IGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDKTEFLWQSSDGSEVTAQVLPL
GYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLPNGHDQMPLQQNIFEVMDKLREI
YPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGKYMRVHRTIGSTRMDIKIAHARIEN
KIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILKNHAHDSIGCCCSDKVHREIVARFELA
EDMADNLIRFYMRKIADNMPQSDADKLVLFNLMPWPREEVINTTVRLRASQFNLRDDRGQ
PVPYFIRHAREIDPGLIDRQIVHYGNYDPFMEFDIQINQIVPSMGYRTLYIEANQPGNVI
AAKSDAEGILENAFWQIALNEDGSLQLVDKDSGVRYDRVLQIEESSDDGDEYDYSPAKEE
WVITAANAKPQCDIIHEAWQSRAVIRYDMAVPLNLSERSARQSTGRVGVVLVVTLSHNSR
RIDVDINLDNQADDHRLRVLVPTPFNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAP
VPVWNMLNYVALQEGRNGMAVFSEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPG
RPSGIKMPVPDSQLRGLLSCRLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDVMKL
NKAGFNVPESYSLLKMPPVGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVIS
CSETMMDEHITTEENQGSNLSGPFLPGQSRTFSYRLA
References
External Links:
ResourceLink
Uniprot ID:P54746
Uniprot Name:MNGB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4062319
Ecogene ID:EG13236
Ecocyc:EG13236
ColiBase:b0732
Kegg Gene:b0732
EchoBASE ID:EB3025
CCDB:MNGB_ECOLI
BacMap:16128707
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Sampaio, M. M., Chevance, F., Dippel, R., Eppler, T., Schlegel, A., Boos, W., Lu, Y. J., Rock, C. O. (2004). "Phosphotransferase-mediated transport of the osmolyte 2-O-alpha-mannosyl-D-glycerate in Escherichia coli occurs by the product of the mngA (hrsA) gene and is regulated by the mngR (farR) gene product acting as repressor." J Biol Chem 279:5537-5548. Pubmed: 14645248
  • Utsumi, R., Horie, T., Katoh, A., Kaino, Y., Tanabe, H., Noda, M. (1996). "Isolation and characterization of the heat-responsive genes in Escherichia coli." Biosci Biotechnol Biochem 60:309-315. Pubmed: 9063979