6-phosphogluconolactonase (P52697)

Identification
Name:6-phosphogluconolactonase
Synonyms:
  • 6-P-gluconolactonase
  • Pgl
Gene Name:pgl
Enzyme Class:
Biological Properties
General Function:Involved in 6-phosphogluconolactonase activity
Specific Function:Catalyzes the hydrolysis of 6-phosphogluconolactone to 6-phosphogluconate
Cellular Location:Cytoplasmic
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.06-Phosphonoglucono-D-lactone + 1.0Water ↔ 1.06-Phosphogluconic acid + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb
1.06-Phosphonoglucono-D-lactone + 1.0Water ↔ 1.06-Phosphogluconic acid
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.06-Phosphonoglucono-D-lactone + 1.0Water ↔ 1.06-Phosphogluconic acid + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.06-Phosphonoglucono-D-lactone + 1.0Water → 1.0Hydrogen ion + 1.06-Phosphogluconic acid
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb
1.06-Phosphonoglucono-D-lactone + 1.0Water → 1.06-Phosphogluconic acid
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB013166-Phosphogluconic acidMetaboCard
ECMDB011276-Phosphonoglucono-D-lactoneMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
6-phosphogluconolactonase activity
carboxylesterase activity
catalytic activity
hydrolase activity
hydrolase activity, acting on ester bonds
Process
alcohol metabolic process
carbohydrate metabolic process
glucose catabolic process
glucose metabolic process
hexose metabolic process
metabolic process
monosaccharide metabolic process
pentose-phosphate shunt
primary metabolic process
small molecule metabolic process
Gene Properties
Blattner:b0767
Gene OrientationClockwise
Centisome Percentage:17.20
Left Sequence End797809
Right Sequence End798804
Gene Sequence:
>996 bp
ATGAAGCAAACAGTTTATATCGCCAGCCCTGAGAGCCAGCAAATTCACGTCTGGAATCTG
AATCATGAAGGCGCACTGACGCTGACACAGGTTGTCGATGTGCCGGGGCAGGTGCAGCCG
ATGGTGGTCAGCCCGGACAAACGTTATCTCTATGTTGGTGTTCGCCCTGAGTTTCGCGTC
CTGGCGTATCGTATCGCCCCGGACGATGGCGCACTGACCTTTGCCGCAGAGTCTGCGCTG
CCGGGTAGTCCGACGCATATTTCCACCGATCACCAGGGGCAGTTTGTCTTTGTAGGTTCT
TACAATGCGGGTAACGTGAGCGTAACGCGTCTGGAAGATGGCCTGCCAGTGGGCGTCGTC
GATGTGGTCGAGGGGCTGGACGGTTGCCATTCCGCCAATATCTCACCGGACAACCGTACG
CTGTGGGTTCCGGCATTAAAGCAGGATCGCATTTGCCTGTTTACGGTCAGCGATGATGGT
CATCTCGTGGCGCAGGACCCTGCGGAAGTGACCACCGTTGAAGGGGCCGGCCCGCGTCAT
ATGGTATTCCATCCAAACGAACAATATGCGTATTGCGTCAATGAGTTAAACAGCTCAGTG
GATGTCTGGGAACTGAAAGATCCGCACGGTAATATCGAATGTGTCCAGACGCTGGATATG
ATGCCGGAAAACTTCTCCGACACCCGTTGGGCGGCTGATATTCATATCACCCCGGATGGT
CGCCATTTATACGCCTGCGACCGTACCGCCAGCCTGATTACCGTTTTCAGCGTTTCGGAA
GATGGCAGCGTGTTGAGTAAAGAAGGCTTCCAGCCAACGGAAACCCAGCCGCGCGGCTTC
AATGTTGATCACAGCGGCAAGTATCTGATTGCCGCCGGGCAAAAATCTCACCACATCTCG
GTATACGAAATTGTTGGCGAGCAGGGGCTACTGCATGAAAAAGGCCGCTATGCGGTCGGG
CAGGGACCAATGTGGGTGGTGGTTAACGCACACTAA
Protein Properties
Pfam Domain Function:
Protein Residues:331
Protein Molecular Weight:36308
Protein Theoretical pI:5
PDB File:1RI6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>6-phosphogluconolactonase
MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVSPDKRYLYVGVRPEFRV
LAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYNAGNVSVTRLEDGLPVGVV
DVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDGHLVAQDPAEVTTVEGAGPRH
MVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTLDMMPENFSDTRWAADIHITPDG
RHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQPRGFNVDHSGKYLIAAGQKSHHIS
VYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH
References
External Links:
ResourceLink
Uniprot ID:P52697
Uniprot Name:6PGL_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4062334
PDB ID:1RI6
Ecogene ID:EG13231
Ecocyc:EG13231
ColiBase:b0767
Kegg Gene:b0767
EchoBASE ID:EB3020
CCDB:6PGL_ECOLI
BacMap:16128735
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Maupin-Furlow, J. A., Rosentel, J. K., Lee, J. H., Deppenmeier, U., Gunsalus, R. P., Shanmugam, K. T. (1995). "Genetic analysis of the modABCD (molybdate transport) operon of Escherichia coli." J Bacteriol 177:4851-4856. Pubmed: 7665460
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Thomason, L. C., Court, D. L., Datta, A. R., Khanna, R., Rosner, J. L. (2004). "Identification of the Escherichia coli K-12 ybhE gene as pgl, encoding 6-phosphogluconolactonase." J Bacteriol 186:8248-8253. Pubmed: 15576773
  • Zhang, J., Sprung, R., Pei, J., Tan, X., Kim, S., Zhu, H., Liu, C. F., Grishin, N. V., Zhao, Y. (2009). "Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli." Mol Cell Proteomics 8:215-225. Pubmed: 18723842