ECMDB: Alpha-ribazole phosphatase (P52086)

Identification

Name:

Alpha-ribazole phosphatase

Synonyms:

Alpha-ribazole-5'-phosphate phosphatase

Gene Name:

cobC

Enzyme Class:

3.1.3.73

Biological Properties

General Function:

Involved in catalytic activity

Specific Function:

Converts N1-(5-phospho-alpha-D-ribosyl)-5,6- dimethylbenzimidazole into N1-alpha-D-ribosyl-5,6- dimethylbenzimidazole; involved in the assembly of the nucleotide loop of cobalamin

Cellular Location:

Cytoplasmic

SMPDB Pathways:

adenosylcobalamin salvage from cobinamide PW001884

KEGG Pathways:

Metabolic pathways eco01100
Porphyrin and chlorophyll metabolism ec00860
Riboflavin metabolism ec00740

KEGG Reactions:

1.0

→

1.0

1.0N1-(5-Phospho-a-D-ribosyl)-5,6-dimethylbenzimidazole + 1.0Water → 1.0Phosphate + 1.0N1-(alpha-D-ribosyl)-5,6-dimethyl-benzimidazole
ReactionCard

1.0

↔

1.0

1.0N1-(5-Phospho-a-D-ribosyl)-5,6-dimethylbenzimidazole + 1.0Water ↔ 1.0N1-(alpha-D-ribosyl)-5,6-dimethyl-benzimidazole + 1.0Phosphate
ReactionCard

SMPDB Reactions:

1.0

→

1.0

1.0coenzyme B12

1.0Adenosylcobalamin 5'-phosphate + 1.0Water → 1.0Phosphate + 1.0coenzyme B12
ReactionCard

EcoCyc Reactions:

1.0

→

1.0

1.0N1-(5-Phospho-a-D-ribosyl)-5,6-dimethylbenzimidazole + 1.0Water → 1.0Phosphate + 1.0N1-(alpha-D-ribosyl)-5,6-dimethyl-benzimidazole
ReactionCard

1.0adenosylcobalamin 5'-phosphate

1.0

→

1.0

1.0adenosylcobalamin 5'-phosphate + 1.0Water → 1.0Adenosylcobalamin + 1.0Phosphate
ReactionCard

Complex Reactions:

1.0

→

1.0

1.0Adenosylcobalamin + 1.0Water → 1.0Adenosylcobalamin + 1.0Inorganic phosphate
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB02086	Adenosylcobalamin	MetaboCard
ECMDB23095	Adenosylcobalamin 5'-phosphate	MetaboCard
ECMDB21380	Inorganic phosphate	MetaboCard
ECMDB03882	N1-(5-Phospho-a-D-ribosyl)-5,6-dimethylbenzimidazole	MetaboCard
ECMDB11112	N1-(alpha-D-ribosyl)-5,6-dimethyl-benzimidazole	MetaboCard
ECMDB01429	Phosphate	MetaboCard
ECMDB00494	Water	MetaboCard

GO Classification:

Function
alpha-ribazole phosphatase activity
catalytic activity
hydrolase activity
hydrolase activity, acting on ester bonds
phosphatase activity
phosphoric ester hydrolase activity
Process
cobalamin biosynthetic process
metabolic process
nitrogen compound metabolic process
porphyrin biosynthetic process
porphyrin metabolic process
tetrapyrrole metabolic process

Gene Properties

Blattner:

b0638

Gene Orientation

Counterclockwise

Centisome Percentage:

14.41

Left Sequence End

668519

Right Sequence End

669130

Gene Sequence:

>612 bp
ATGCGACTGTGGTTAATTCGTCATGGTGAAACGCAAGCGAATATCGATGGTCTTTACAGC
GGTCATGCGCCCACCCCCCTGACCGCGCGCGGTATTGAGCAAGCGCAAAATCTGCATACG
CTGCTACATGGTGTTTCCTTTGATCTGGTTTTATGCAGTGAACTGGAACGGGCACAGCAT
ACCGCGCGACTGGTTCTCAGTGACCGCCAGCTCCCCGTGCAAATCATACCTGAACTCAAC
GAAATGTTTTTTGGCGACTGGGAGATGCGACATCATCGCGACCTCATGCAAGAAGATGCC
GAAAACTATAGCGCGTGGTGCAATGACTGGCAGCATGCAATCCCCACGAACGGTGAAGGA
TTTCAGGCATTTTCGCAACGTGTGGAACGCTTTATCGCAAGGCTTAGTGAATTTCAGCAC
TATCAGAATATTTTAGTCGTCAGCCATCAGGGTGTACTGAGTCTGTTAATCGCCCGTTTA
ATTGGCATGCCTGCCGAAGCTATGTGGCATTTTCGCGTTGACCAGGGGTGTTGGAGCGCC
ATTGATATCAACCAAAAATTCGCGACGCTACGCGTCCTCAATAGCCGTGCCATCGGGGTC
GAGAATGCATGA

Protein Properties

Pfam Domain Function:

PGAM (PF00300 )

Protein Residues:

203

Protein Molecular Weight:

23308

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>Alpha-ribazole phosphatase
MRLWLIRHGETQANIDGLYSGHAPTPLTARGIEQAQNLHTLLHGVSFDLVLCSELERAQH
TARLVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAENYSAWCNDWQHAIPTNGEG
FQAFSQRVERFIARLSEFQHYQNILVVSHQGVLSLLIARLIGMPAEAMWHFRVDQGCWSA
IDINQKFATLRVLNSRAIGVENA

References

External Links:

Resource	Link
Uniprot ID:	P52086
Uniprot Name:	COBC_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	4062256
Ecogene ID:	EG13240
Ecocyc:	EG13240
ColiBase:	b0638
Kegg Gene:	b0638
EchoBASE ID:	EB3029
CCDB:	COBC_ECOLI
BacMap:	16128621

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232