S-ribosylhomocysteine lyase (P45578)

Identification
Name:S-ribosylhomocysteine lyase
Synonyms:
  • AI-2 synthesis protein
  • Autoinducer-2 production protein luxS
Gene Name:luxS
Enzyme Class:
Biological Properties
General Function:Involved in catalytic activity
Specific Function:Involved in the synthesis of autoinducer 2 (AI-2) which is secreted by bacteria and is used to communicate both the cell density and the metabolic potential of the environment. The regulation of gene expression in response to changes in cell density is called quorum sensing. Catalyzes the transformation of S-ribosylhomocysteine (RHC) to homocysteine (HC) and 4,5- dihydroxy-2,3-pentadione (DPD)
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0S-Ribosyl-L-homocysteine ↔ 1.0(4S)-4,5-Dihydroxypentan-2,3-dione + 1.0L-Homocysteine
ReactionCard
SMPDB Reactions:
1.0 S-ribosyl-L-homocysteine+1.0Thumb → 1.0Homocysteine+1.0autoinducer 2+1.0Thumb+1.0Thumb
1.0 S-ribosyl-L-homocysteine + 1.0 S-ribosyl-L-homocysteine → 1.0Homocysteine + 1.0autoinducer 2 + 1.0Homocysteine + 1.0autoinducer 2
ReactionCard
1.0Thumb → 1.0Thumb+1.0Thumb
1.0 S-ribosyl-L-homocysteine → 1.04,5-Dihydroxy-2,3-pentanedione + 1.0Homocysteine
ReactionCard
EcoCyc Reactions:
1.0Thumb → 1.0Thumb+1.0Thumb
1.0S-Ribosyl-L-homocysteine → 1.04,5-Dihydroxy-2,3-pentanedione + 1.0L-Homocysteine
ReactionCard
Complex Reactions:
1.0Thumb → 1.0Thumb+1.0Thumb
1.0S-Ribosyl-L-homocysteine → 1.0L-Homocysteine + 1.0(4S)-4,5-Dihydroxypentan-2,3-dione
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB24231 S-ribosyl-L-homocysteineMetaboCard
ECMDB21640(4S)-4,5-Dihydroxypentan-2,3-dioneMetaboCard
ECMDB205504,5-Dihydroxy-2,3-pentanedioneMetaboCard
ECMDB24232autoinducer 2MetaboCard
ECMDB24053HomocysteineMetaboCard
ECMDB00165L-HomocysteineMetaboCard
ECMDB20190S-Ribosyl-L-homocysteineMetaboCard
GO Classification:
Function
binding
catalytic activity
cation binding
ion binding
iron ion binding
metal ion binding
transition metal ion binding
Process
biological regulation
homeostasis of number of cells
homeostasis of number of cells in a free-living population
homeostatic process
quorum sensing
regulation of biological quality
Gene Properties
Blattner:b2687
Gene OrientationCounterclockwise
Centisome Percentage:60.61
Left Sequence End2812240
Right Sequence End2812755
Gene Sequence:
>516 bp
ATGAGAAGAGTACTCTTTAGCTGTTTCTGCGGGCTACTGTGGAGTTCCAGTGGATGGGCA
GTTGACCCTTTAGGAACGATTAATATCAATTTGCACGGTAACGTTGTTGATTTCTCCTGT
ACCGTAAACACAGCGGATATTGATAAGACGGTAGATTTAGGCAGATGGCCTACGACACAA
CTACTGAACGCTGGCGATACCACGGCACTCGTCCCTTTTAGCCTGCGGCTGGAGGGATGT
CCTCCGGGTTCAGTTGCGATTTTATTTACGGGAACGCCGGCATCCGATACCAACCTGCTG
GCTCTGGATGATCCCGCAATGGCACAAACCGTCGCCATCGAATTACGTAATAGCGATCGC
TCCCGGCTCGCACTGGGGGAGGCGAGCCCGACTGAGGAAGTAGATGCAAATGGCAATGTC
ACACTAAACTTTTTTGCCAATTATCGAGCGTTAGCCAGCGGTGTTCGGCCAGGTGTGGCG
AAAGCGGATGCGATATTTATGATCAATTATAATTAA
Protein Properties
Pfam Domain Function:
Protein Residues:171
Protein Molecular Weight:19416
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>S-ribosylhomocysteine lyase
MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPNKEVMPERGIHTLEHLF
AGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVADAWKAAMEDVLKVQDQNQI
PELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELALPKEKLQELHI
References
External Links:
ResourceLink
Uniprot ID:P45578
Uniprot Name:LUXS_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674671
Ecogene ID:EG12712
Ecocyc:EG12712
ColiBase:b2687
Kegg Gene:b2687
EchoBASE ID:EB2573
CCDB:LUXS_ECOLI
BacMap:16130599
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Borodovsky, M., McIninch, J. D., Koonin, E. V., Rudd, K. E., Medigue, C., Danchin, A. (1995). "Detection of new genes in a bacterial genome using Markov models for three gene classes." Nucleic Acids Res 23:3554-3562. Pubmed: 7567469
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Link, A. J., Robison, K., Church, G. M. (1997). "Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12." Electrophoresis 18:1259-1313. Pubmed: 9298646
  • Lomovskaya, O., Lewis, K. (1992). "Emr, an Escherichia coli locus for multidrug resistance." Proc Natl Acad Sci U S A 89:8938-8942. Pubmed: 1409590
  • Surette, M. G., Bassler, B. L. (1998). "Quorum sensing in Escherichia coli and Salmonella typhimurium." Proc Natl Acad Sci U S A 95:7046-7050. Pubmed: 9618536
  • Surette, M. G., Miller, M. B., Bassler, B. L. (1999). "Quorum sensing in Escherichia coli, Salmonella typhimurium, and Vibrio harveyi: a new family of genes responsible for autoinducer production." Proc Natl Acad Sci U S A 96:1639-1644. Pubmed: 9990077
  • Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837