Glutathione-regulated potassium-efflux system protein kefB (P45522)

Identification
Name:Glutathione-regulated potassium-efflux system protein kefB
Synonyms:
  • K(+)/H(+) antiporter
  • NEM-activable K(+)/H(+) antiporter
Gene Name:kefB
Enzyme Class:Not Available
Biological Properties
General Function:Involved in solute:hydrogen antiporter activity
Specific Function:Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Transports:
Transport References:
  • Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
Metabolites:
ECMDB IDNameView
ECMDB21225Hydrogen ionMetaboCard
ECMDB04139PotassiumMetaboCard
GO Classification:
Component
cell part
integral to membrane
intrinsic to membrane
membrane
membrane part
plasma membrane
Function
binding
catalytic activity
cation transmembrane transporter activity
glutathione-regulated potassium exporter activity
inorganic cation transmembrane transporter activity
ion transmembrane transporter activity
monovalent inorganic cation transmembrane transporter activity
potassium ion transmembrane transporter activity
solute:cation antiporter activity
solute:hydrogen antiporter activity
substrate-specific transmembrane transporter activity
transmembrane transporter activity
transporter activity
Process
cation transport
establishment of localization
ion transport
metabolic process
monovalent inorganic cation transport
potassium ion transport
transmembrane transport
transport
Gene Properties
Blattner:b3350
Gene OrientationCounterclockwise
Centisome Percentage:74.94
Left Sequence End3476824
Right Sequence End3478629
Gene Sequence:
>1806 bp
ATGGAAGGTTCCGATTTTTTACTCGCAGGAGTGCTGTTTCTCTTCGCGGCGGTGGCTGCG
GTGCCGCTGGCATCGCGGCTGGGTATTGGCGCTGTGTTGGGATATTTGCTGGCAGGGATT
GCAATTGGCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCG
GAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGAATCCCTCCAAACTT
TGGCAACTGCGGCGTTCGATTTTTGGCGTAGGCGCGGCACAGGTGCTGTTAAGCGCGGCG
TTGCTGGCGGGATTATTGATGCTGACGGATTTCGCCTGGCAGGCGGCGGTGGTCGGTGGC
ATTGGCCTTGCGATGTCTTCAACTGCAATGGCGTTGCAATTGATGCGTGAGAAAGGGATG
AATCGCAGCGAATCCGGCCAGCTCGGGTTTTCGGTTCTGCTGTTTCAGGATCTGGCAGTA
ATCCCAGCACTGGCGTTAGTGCCGTTGTTGGCGGGGTCGGCAGACGAACATTTCGACTGG
ATGAAGGTCGGCATGAAGGTGCTGGCGTTTGTCGGCATGCTGATTGGTGGGCGCTATTTA
CTGCGTCCGGTATTCCGCTTTATTGCAGCTTCTGGCGTGCGGGAAGTGTTCACCGCCGCG
ACGCTGCTGCTGGTGTTGGGTTCCGCATTGTTTATGGATGCGCTGGGGCTGTCGATGGCG
CTCGGTACATTTATTGCGGGCGTGCTGCTGGCGGAAAGTGAATATCGCCATGAACTGGAA
ACGGCTATCGATCCCTTCAAAGGCTTGCTGCTCGGTTTGTTCTTTATCTCTGTCGGCATG
TCACTCAACCTCGGGGTGCTTTATACCCATCTGTTGTGGGTAGTGATAAGCGTGGTTGTG
CTGGTGGCGGTGAAAATTCTCGTGCTGTATCTGCTGGCGCGATTGTATGGCGTGCGTAGC
TCAGAGCGGATGCAGTTTGCTGGCGTGTTGAGTCAGGGTGGTGAGTTTGCCTTTGTCCTC
TTTTCTACCGCTTCTTCACAACGCTTATTCCAGGGCGACCAGATGGCGTTGTTGCTGGTG
ACGGTGACGCTTTCCATGATGACCACGCCGTTGCTGATGAAGCTGGTGGATAAATGGCTA
TCCCGCCAGTTTAACGGACCGGAAGAAGAAGACGAAAAACCGTGGGTCAACGATGATAAA
CCCCAGGTCATTGTCGTGGGCTTCGGGCGTTTTGGTCAGGTGATTGGTCGTTTGCTGATG
GCAAATAAAATGCGCATTACCGTGCTGGAGCGGGATATCAGCGCCGTTAACCTGATGCGT
AAATACGGCTACAAAGTTTATTACGGCGACGCCACGCAGGTCGATCTTTTACGTTCTGCG
GGTGCAGAGGCCGCTGAGTCTATCGTCATTACCTGTAACGAGCCGGAAGACACCATGAAG
CTGGTGGAAATATGCCAACAGCACTTTCCGCATTTGCATATTCTTGCGCGAGCGCGCGGA
CGTGTGGAAGCGCATGAGTTATTACAGGCAGGGGTGACGCAGTTTTCCCGTGAAACATTC
TCCAGTGCGTTAGAGCTGGGGCGCAAGACGCTGGTCACGCTTGGCATGCATCCGCATCAG
GCACAGCGAGCGCAACTGCATTTTCGCCGCCTGGATATGCGGATGCTGCGAGAGCTCATC
CCAATGCATGCCGATACCGTACAAATTTCTCGCGCCAGGGAAGCCCGACGCGAACTGGAA
GAGATTTTCCAGCGTGAAATGCAACAAGAACGACGCCAGCTGGACGGCTGGGATGAATTT
GAGTAG
Protein Properties
Pfam Domain Function:
Protein Residues:601
Protein Molecular Weight:66411
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • 5-24
  • 29-51
  • 56-73
  • 86-108
  • 113-135
  • 148-170
  • 180-197
  • 204-226
  • 230-252
  • 265-287
  • 291-313
  • 326-344
  • 354-376
Protein Sequence:
>Glutathione-regulated potassium-efflux system protein kefB
MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGPWGLGFISDVDEILHFS
ELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALLAGLLMLTDFAWQAAVVGG
IGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAVIPALALVPLLAGSADEHFDW
MKVGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFTAATLLLVLGSALFMDALGLSMA
LGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFISVGMSLNLGVLYTHLLWVVISVVV
LVAVKILVLYLLARLYGVRSSERMQFAGVLSQGGEFAFVLFSTASSQRLFQGDQMALLLV
TVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDEKPWVNDDKPQVIVVGFGRFGQVIGRLLM
ANKMRITVLERDISAVNLMRKYGYKVYYGDATQVDLLRSAGAEAAESIVITCNEPEDTMK
LVEICQQHFPHLHILARARGRVEAHELLQAGVTQFSRETFSSALELGRKTLVTLGMHPHQ
AQRAQLHFRRLDMRMLRELIPMHADTVQISRAREARRELEEIFQREMQQERRQLDGWDEF
E
References
External Links:
ResourceLink
Uniprot ID:P45522
Uniprot Name:KEFB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85676690
Ecogene ID:EG20110
Ecocyc:EG20110
ColiBase:b3350
Kegg Gene:b3350
EchoBASE ID:EB4144
CCDB:KEFB_ECOLI
BacMap:16131229
General Reference:
  • Bakker, E. P., Booth, I. R., Dinnbier, U., Epstein, W., Gajewska, A. (1987). "Evidence for multiple K+ export systems in Escherichia coli." J Bacteriol 169:3743-3749. Pubmed: 3301813
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553