| Identification |
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| Name: | Glutathione-regulated potassium-efflux system protein kefB |
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| Synonyms: | - K(+)/H(+) antiporter
- NEM-activable K(+)/H(+) antiporter
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| Gene Name: | kefB |
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| Enzyme Class: | Not Available |
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| Biological Properties |
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| General Function: | Involved in solute:hydrogen antiporter activity |
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| Specific Function: | Transport system that facilitates potassium-efflux, possibly by potassium-proton antiport |
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| Cellular Location: | Cell inner membrane; Multi-pass membrane protein |
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| SMPDB Pathways: | Not Available |
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| KEGG Pathways: | Not Available |
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| Transports: | |
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| Transport References: | - Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
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| Metabolites: | |
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| GO Classification: | | Component |
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| cell part | | integral to membrane | | intrinsic to membrane | | membrane | | membrane part | | plasma membrane | | Function |
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| binding | | catalytic activity | | cation transmembrane transporter activity | | glutathione-regulated potassium exporter activity | | inorganic cation transmembrane transporter activity | | ion transmembrane transporter activity | | monovalent inorganic cation transmembrane transporter activity | | potassium ion transmembrane transporter activity | | solute:cation antiporter activity | | solute:hydrogen antiporter activity | | substrate-specific transmembrane transporter activity | | transmembrane transporter activity | | transporter activity | | Process |
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| cation transport | | establishment of localization | | ion transport | | metabolic process | | monovalent inorganic cation transport | | potassium ion transport | | transmembrane transport | | transport |
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| Gene Properties |
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| Blattner: | b3350 |
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| Gene Orientation | Counterclockwise |
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| Centisome Percentage: | 74.94 |
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| Left Sequence End | 3476824 |
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| Right Sequence End | 3478629 |
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| Gene Sequence: | >1806 bp
ATGGAAGGTTCCGATTTTTTACTCGCAGGAGTGCTGTTTCTCTTCGCGGCGGTGGCTGCG
GTGCCGCTGGCATCGCGGCTGGGTATTGGCGCTGTGTTGGGATATTTGCTGGCAGGGATT
GCAATTGGCCCGTGGGGGCTGGGGTTTATTAGCGACGTCGATGAGATCCTCCACTTTTCG
GAACTCGGCGTGGTATTCCTGATGTTTATCATCGGCCTTGAGTTGAATCCCTCCAAACTT
TGGCAACTGCGGCGTTCGATTTTTGGCGTAGGCGCGGCACAGGTGCTGTTAAGCGCGGCG
TTGCTGGCGGGATTATTGATGCTGACGGATTTCGCCTGGCAGGCGGCGGTGGTCGGTGGC
ATTGGCCTTGCGATGTCTTCAACTGCAATGGCGTTGCAATTGATGCGTGAGAAAGGGATG
AATCGCAGCGAATCCGGCCAGCTCGGGTTTTCGGTTCTGCTGTTTCAGGATCTGGCAGTA
ATCCCAGCACTGGCGTTAGTGCCGTTGTTGGCGGGGTCGGCAGACGAACATTTCGACTGG
ATGAAGGTCGGCATGAAGGTGCTGGCGTTTGTCGGCATGCTGATTGGTGGGCGCTATTTA
CTGCGTCCGGTATTCCGCTTTATTGCAGCTTCTGGCGTGCGGGAAGTGTTCACCGCCGCG
ACGCTGCTGCTGGTGTTGGGTTCCGCATTGTTTATGGATGCGCTGGGGCTGTCGATGGCG
CTCGGTACATTTATTGCGGGCGTGCTGCTGGCGGAAAGTGAATATCGCCATGAACTGGAA
ACGGCTATCGATCCCTTCAAAGGCTTGCTGCTCGGTTTGTTCTTTATCTCTGTCGGCATG
TCACTCAACCTCGGGGTGCTTTATACCCATCTGTTGTGGGTAGTGATAAGCGTGGTTGTG
CTGGTGGCGGTGAAAATTCTCGTGCTGTATCTGCTGGCGCGATTGTATGGCGTGCGTAGC
TCAGAGCGGATGCAGTTTGCTGGCGTGTTGAGTCAGGGTGGTGAGTTTGCCTTTGTCCTC
TTTTCTACCGCTTCTTCACAACGCTTATTCCAGGGCGACCAGATGGCGTTGTTGCTGGTG
ACGGTGACGCTTTCCATGATGACCACGCCGTTGCTGATGAAGCTGGTGGATAAATGGCTA
TCCCGCCAGTTTAACGGACCGGAAGAAGAAGACGAAAAACCGTGGGTCAACGATGATAAA
CCCCAGGTCATTGTCGTGGGCTTCGGGCGTTTTGGTCAGGTGATTGGTCGTTTGCTGATG
GCAAATAAAATGCGCATTACCGTGCTGGAGCGGGATATCAGCGCCGTTAACCTGATGCGT
AAATACGGCTACAAAGTTTATTACGGCGACGCCACGCAGGTCGATCTTTTACGTTCTGCG
GGTGCAGAGGCCGCTGAGTCTATCGTCATTACCTGTAACGAGCCGGAAGACACCATGAAG
CTGGTGGAAATATGCCAACAGCACTTTCCGCATTTGCATATTCTTGCGCGAGCGCGCGGA
CGTGTGGAAGCGCATGAGTTATTACAGGCAGGGGTGACGCAGTTTTCCCGTGAAACATTC
TCCAGTGCGTTAGAGCTGGGGCGCAAGACGCTGGTCACGCTTGGCATGCATCCGCATCAG
GCACAGCGAGCGCAACTGCATTTTCGCCGCCTGGATATGCGGATGCTGCGAGAGCTCATC
CCAATGCATGCCGATACCGTACAAATTTCTCGCGCCAGGGAAGCCCGACGCGAACTGGAA
GAGATTTTCCAGCGTGAAATGCAACAAGAACGACGCCAGCTGGACGGCTGGGATGAATTT
GAGTAG |
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| Protein Properties |
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| Pfam Domain Function: | |
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| Protein Residues: | 601 |
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| Protein Molecular Weight: | 66411 |
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| Protein Theoretical pI: | 7 |
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| Signaling Regions: | |
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| Transmembrane Regions: | - 5-24
- 29-51
- 56-73
- 86-108
- 113-135
- 148-170
- 180-197
- 204-226
- 230-252
- 265-287
- 291-313
- 326-344
- 354-376
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| Protein Sequence: | >Glutathione-regulated potassium-efflux system protein kefB
MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGPWGLGFISDVDEILHFS
ELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALLAGLLMLTDFAWQAAVVGG
IGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAVIPALALVPLLAGSADEHFDW
MKVGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFTAATLLLVLGSALFMDALGLSMA
LGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFISVGMSLNLGVLYTHLLWVVISVVV
LVAVKILVLYLLARLYGVRSSERMQFAGVLSQGGEFAFVLFSTASSQRLFQGDQMALLLV
TVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDEKPWVNDDKPQVIVVGFGRFGQVIGRLLM
ANKMRITVLERDISAVNLMRKYGYKVYYGDATQVDLLRSAGAEAAESIVITCNEPEDTMK
LVEICQQHFPHLHILARARGRVEAHELLQAGVTQFSRETFSSALELGRKTLVTLGMHPHQ
AQRAQLHFRRLDMRMLRELIPMHADTVQISRAREARRELEEIFQREMQQERRQLDGWDEF
E |
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| References |
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| External Links: | |
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| General Reference: | - Bakker, E. P., Booth, I. R., Dinnbier, U., Epstein, W., Gajewska, A. (1987). "Evidence for multiple K+ export systems in Escherichia coli." J Bacteriol 169:3743-3749. Pubmed: 3301813
- Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
- Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
- Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
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