Probable low-affinity inorganic phosphate transporter 2 (P43676)

Identification
Name:Probable low-affinity inorganic phosphate transporter 2
Synonyms:Not Available
Gene Name:pitB
Enzyme Class:Not Available
Biological Properties
General Function:Involved in inorganic phosphate transmembrane transporter activity
Specific Function:Low-affinity inorganic phosphate transport
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Transports:
Transport References:
  • Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
Metabolites:
ECMDB IDNameView
ECMDB21225Hydrogen ionMetaboCard
ECMDB01429PhosphateMetaboCard
GO Classification:
Component
cell part
membrane
Function
anion transmembrane transporter activity
inorganic anion transmembrane transporter activity
inorganic phosphate transmembrane transporter activity
ion transmembrane transporter activity
phosphate transmembrane transporter activity
substrate-specific transmembrane transporter activity
transmembrane transporter activity
transporter activity
Process
anion transport
establishment of localization
inorganic anion transport
ion transport
phosphate transport
transport
Gene Properties
Blattner:b2987
Gene OrientationCounterclockwise
Centisome Percentage:67.52
Left Sequence End3132894
Right Sequence End3134393
Gene Sequence:
>1500 bp
ATGACAGAGCCGCATGTAGCAGTATTAAGCCAGGTCCAACAGTTTCTCGATCGTCAACAC
GGTCTTTATATTGATGGTCGTCCTGGCCCCGCACAAAGTGAAAAACGGTTGGCGATCTTT
GATCCGGCCACCGGGCAAGAAATTGCGTCTACTGCTGATGCCAACGAAGCGGATGTAGAT
AACGCAGTCATGTCTGCCTGGCGGGCCTTTGTCTCGCGTCGCTGGGCCGGGCGATTACCC
GCAGAGCGTGAACGTATTCTGCTACGTTTTGCTGATCTGGTGGAGCAGCACAGTGAGGAG
CTGGCGCAACTGGAAACCCTGGAGCAAGGCAAGTCAATTGCCATTTCCCGTGCTTTTGAA
GTGGGCTGTACGCTGAACTGGATGCGTTATACCGCCGGGTTAACGACCAAAATCGCGGGT
AAAACGCTGGACTTGTCGATTCCCTTACCCCAGGGGGCGCGTTATCAGGCCTGGACGCGT
AAAGAGCCGGTTGGCGTAGTGGCGGGAATTGTGCCATGGAACTTTCCGTTGATGATTGGT
ATGTGGAAGGTGATGCCAGCACTGGCAGCAGGCTGTTCAATCGTGATTAAGCCTTCGGAA
ACCACGCCACTGACGATGTTGCGCGTGGCGGAACTGGCCAGCGAGGCTGGTATCCCTGAT
GGCGTTTTTAATGTCGTCACCGGGTCAGGTGCTGTATGCGGCGCGGCCCTGACGTCACAT
CCTCATGTTGCGAAAATCAGTTTTACCGGTTCAACCGCGACGGGAAAAGGTATTGCCAGA
ACTGCTGCTGATCACTTAACGCGTGTAACGCTGGAACTGGGCGGTAAAAACCCGGCAATT
GTATTAAAAGATGCTGATCCGCAATGGGTTATTGAAGGCTTGATGACCGGAAGCTTCCTG
AATCAAGGGCAAGTATGCGCCGCCAGTTCGCGAATTTATATTGAAGCGCCGTTGTTTGAC
ACGCTGGTTAGTGGATTTGAGCAGGCGGTAAAATCGTTGCAAGTGGGACCGGGGATGTCA
CCTGTTGCACAGATTAACCCTTTGGTTTCTCGTGCGCACTGCGACAAAGTGTGTTCATTC
CTCGACGATGCGCAGGCACAGCAAGCAGAGCTGATTCGCGGGTCGAATGGACCAGCCGGA
GAGGGGTATTATGTTGCGCCAACGCTGGTGGTAAATCCCGATGCTAAATTGCGCTTAACT
CGTGAAGAGGTGTTTGGTCCGGTGGTAAACCTGGTGCGAGTAGCGGATGGAGAAGAGGCG
TTACAACTGGCAAACGACACGGAATATGGCTTAACTGCCAGTGTCTGGACGCAAAATCTC
TCCCAGGCTCTGGAATATAGCGATCGCTTACAGGCAGGGACGGTGTGGGTAAACAGCCAT
ACCTTAATTGACGCTAACTTACCGTTTGGTGGGATGAAGCAGTCAGGAACGGGCCGTGAT
TTTGGCCCCGACTGGCTGGACGGTTGGTGTGAAACTAAGTCGGTGTGTGTACGGTATTAA
Protein Properties
Pfam Domain Function:
Protein Residues:499
Protein Molecular Weight:53808
Protein Theoretical pI:10
Signaling Regions:
  • None
Transmembrane Regions:
  • 5-25
  • 52-72
  • 94-114
  • 124-144
  • 155-175
  • 207-227
  • 233-253
  • 382-402
  • 430-450
  • 473-493
Protein Sequence:
>Probable low-affinity inorganic phosphate transporter 2
MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVAAVIYTRAMQPQLAVVMAAFF
NFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLLAAIIWNLGTWFFGLPASS
SHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLIVSPIVGLVIAGGLIFLLRRY
WSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSAAGVAFSHGANDGQKGIGLVMLV
LVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQHPELPQKLIAMEPPLPAASTDGTQ
VTEFHCHPANTFDAIARVKTMLPGNMESYEPLSVSQRSQLRRIMLCISDTSAKLAKLPGV
SKEDQNLLKKLRSDMLSTIEYAPVWIIMAVALALGIGTMIGWRRVAMTIGEKIGKRGMTY
AQGMAAQMTAAVSIGLASYIGMPVSTTHVLSSAVAGTMVVDGGGLQRKTVTSILMAWVFT
LPAAIFLSGGLYWIALQLI
References
External Links:
ResourceLink
Uniprot ID:P43676
Uniprot Name:PITB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674936
Ecogene ID:EG12883
Ecocyc:EG12883
ColiBase:b2987
Kegg Gene:b2987
EchoBASE ID:EB2721
CCDB:PITB_ECOLI
BacMap:16130887
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Bollinger, J. M. Jr, Kwon, D. S., Huisman, G. W., Kolter, R., Walsh, C. T. (1995). "Glutathionylspermidine metabolism in Escherichia coli. Purification, cloning, overproduction, and characterization of a bifunctional glutathionylspermidine synthetase/amidase." J Biol Chem 270:14031-14041. Pubmed: 7775463
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553