ECMDB

Identification

Name:

ATP-dependent RNA helicase hrpA

Synonyms:

Not Available

Gene Name:

hrpA

Enzyme Class:

3.6.4.13

Biological Properties

General Function:

Replication, recombination and repair

Specific Function:

Not yet known

Cellular Location:

Cytoplasmic

SMPDB Pathways:

Not Available

KEGG Pathways:

Not Available

Metabolites:

ECMDB ID	Name	View
ECMDB00538	Adenosine triphosphate	MetaboCard
ECMDB01341	ADP	MetaboCard
ECMDB21380	Inorganic phosphate	MetaboCard
ECMDB00494	Water	MetaboCard

GO Classification:

Function
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
ATP-dependent helicase activity
ATPase activity
ATPase activity, coupled
binding
catalytic activity
helicase activity
hydrolase activity
hydrolase activity, acting on acid anhydrides
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides
nucleic acid binding
nucleoside binding
nucleoside-triphosphatase activity
nucleotide binding
purine nucleoside binding
pyrophosphatase activity

Gene Properties

Blattner:

Not Available

Gene Orientation

Not Available

Centisome Percentage:

Not Available

Left Sequence End

Not Available

Right Sequence End

Not Available

Gene Sequence:

>3903 bp
ATGACAGAACAACAAAAATTGACCTTTACGGCCTTGCAGCAGCGGCTGGATTCGCTGATG
CTGCGTGACAGACTGCGTTTTTCTCGCCGTCTGCACGGCGTGAAGAAGGTTAAAAATCCT
GATGCACAACAGGCCATTTTCCAGGAGATGGCGAAAGAGATTGACCAGGCGGCAGGGAAA
GTCCTGCTGCGTGAAGCGGCACGACCGGAAATTACTTATCCTGACAATTTACCGGTTAGT
CAGAAAAAACAGGACATTCTCGAAGCGATTCGTGATCACCAGGTGGTGATCGTCGCCGGG
GAAACGGGTTCTGGTAAAACGACTCAGTTACCGAAAATCTGTATGGAGCTGGGGCGCGGG
ATTAAAGGACTGATCGGCCATACCCAGCCGCGTCGTCTGGCGGCAAGAACAGTGGCGAAC
CGTATTGCGGAAGAGCTGAAAACGGAGCCGGGCGGTTGCATCGGTTACAAAGTGCGTTTC
AGCGATCACGTAAGTGATAACACGATGGTCAAGCTGATGACCGACGGTATCCTGCTGGCG
GAGATCCAGCAAGACCGCCTGCTGATGCAGTACGACACTATCATTATTGACGAAGCGCAC
GAACGCAGCCTGAATATCGATTTTTTGCTCGGCTATTTGAAAGAGTTGCTGCCGCGGCGT
CCTGACCTAAAAATCATTATCACTTCCGCGACTATCGACCCGGAACGCTTTTCGCGCCAC
TTTAATAATGCGCCGATTATTGAAGTCTCCGGTCGGACCTATCCGGTGGAAGTGCGCTAT
CGCCCGATTGTTGAAGAAGCCGATGACACCGAGCGCGATCAGTTGCAGGCGATTTTTGAC
GCCGTAGACGAACTGAGTCAGGAAAGCCATGGCGACATTCTGATCTTTATGAGCGGCGAG
CGGGAAATCCGCGATACCGCCGATGCGCTGAACAAGCTGAACTTACGCCATACCGAAATC
TTGCCGCTTTATGCGCGGCTTTCGAACAGCGAACAAAATAGGGTATTCCAGTCGCACAGC
GGACGGCGCATTGTGCTGGCGACCAACGTCGCGGAAACGTCGCTGACCGTACCGGGGATT
AAATACGTTATCGACCCCGGTACAGCGCGTATCAGCCGCTACAGCTATCGCACCAAAGTG
CAGCGTTTGCCGATTGAGCCGATTTCCCAGGCGTCTGCCAATCAGCGTAAAGGCCGCTGT
GGTCGTGTGTCCGAAGGGATCTGTATTCGTCTCTATTCCGAAGACGATTTCCTCTCGCGC
CCGGAGTTTACCGATCCGGAGATTCTGCGTACCAACCTGGCCTCGGTTATTTTGCAGATG
ACCGCGCTGGGGCTGGGCGATATCGCTGCGTTCCCGTTTGTCGAAGCACCGGATAAACGC
AATATCCAGGATGGCGTGCGTCTGCTCGAAGAGCTGGGCGCGATCACCACTGATGAACAG
GCCAGCGCCTATAAACTGACGCCGCTCGGTCGCCAGCTCTCGCAGTTGCCTGTCGACCCA
CGTCTGGCGCGTATGGTGCTGGAAGCGCAAAAACATGGCTGCGTGCGTGAGGCGATGATT
ATCACGTCCGCGCTCTCCATTCAGGATCCGCGCGAACGTCCGATGGACAAACAGCAGGCA
TCGGACGAAAAACATCGTCGCTTCCACGACAAAGAGTCTGACTTTCTCGCGTTTGTGAAT
CTGTGGAATTATCTTGGCGAGCAGCAAAAGGCGCTTTCTTCCAACGCCTTCCGTCGCCTG
TGTCGTACCGATTATCTCAACTATCTGCGCGTGCGCGAATGGCAGGATATCTACACCCAG
TTGCGTCAGGTGGTGAAAGAACTTGGCATTCCGGTTAACAGCGAACCGGCGGAGTATCGC
GAAATTCACATTGCGTTGCTGACCGGTTTACTTTCCCATATCGGCATGAAAGATGCCGAT
AAACAAGAATATACCGGCGCACGTAACGCGCGTTTCTCCATCTTCCCCGGTTCTGGTTTA
TTCAAAAAACCGCCTAAATGGGTAATGGTGGCGGAACTGGTAGAAACCAGCCGCCTGTGG
GGGCGCATTGCTGCGCGTATCGACCCGGAATGGGTGGAGCCAGTTGCTCAGCATTTGATT
AAACGCACCTACAGCGAACCGCACTGGGAACGGGCGCAGGGCGCGGTGATGGCAACGGAA
AAAGTCACTGTTTATGGTTTGCCGATTGTTGCCGCGCGCAAGGTCAACTACAGCCAGATC
GATCCGGCGTTATGTCGTGAACTCTTTATTCGCCACGCGCTGGTGGAAGGTGACTGGCAG
ACGCGTCACGCATTCTTCCGTGAAAACCTGAAACTACGGGCGGAAGTAGAAGAGCTGGAA
CACAAATCACGTCGCCGCGATATTCTGGTTGATGACGAAACGTTGTTTGAGTTCTACGAC
CAGCGCATCAGCCACGATGTAATCTCCGCTCGCCACTTCGACAGCTGGTGGAAAAAAGTC
AGCCGCGAAACGCCTGATTTGCTCAACTTTGAAAAAAGCATGTTGATCAAAGAGGGCGCA
GAAAAAATCAGCAAGCTGGATTACCCGAACTTCTGGCATCAGGGCAATCTCAAGCTGCGT
TTGAGCTATCAGTTTGAGCCCGGCGCGGATGCTGACGGTGTGACCGTACATATTCCGCTG
CCGTTACTTAACCAGGTTGAGGAAAGCGGGTTTGAATGGCAGATCCCCGGTCTGCGCCGC
GAACTGGTGATTGCTCTGATTAAATCGTTGCCGAAACCGGTACGCCGTAATTTTGTACCC
GCGCCAAACTATGCCGAAGCGTTTTTAGGCCGCGTCAAACCGCTGGAGTTACCGTTGCTC
GACAGCCTTGAGCGCGAGTTACGGCGGATGACCGGCGTTACCGTTGACCGCGAAGACTGG
CACTGGGATCAGGTGCCCGATCACCTGAAAATTACCTTCCGCGTGGTGGATGACAAAAAC
AAGAAGCTAAAAGAAGGGCGCTCGCTACAAGATCTGAAAGATGCGCTGAAAGGCAAAGTG
CAGGAAACGCTATCTGCGGTGGCGGATGACGGTATCGAGCAGAGCGGCTTACATATCTGG
AGTTTTGGTCAGCTGCCGGAAAGCTACGAACAGAAGCGTGGCAACTACAAAGTGAAGGCG
TGGCCGGCGCTGGTGGATGAGCGCGACAGTGTGGCGATCAAACTGTTTGATAACCCGCTG
GAGCAAAAGCAGGCAATGTGGAACGGTCTTCGCCGTCTACTGCTGCTGAATATTCCATCG
CCAATCAAATATTTACATGAAAAGTTACCGAACAAAGCCAAGCTGGGACTGTACTTTAAC
CCGTATGGCAAAGTGCTGGAGCTGATCGACGACTGTATCTCCTGCGGTGTGGATAAATTG
ATCGACGCCAATGGTGGCCCGGTCTGGACGGAAGAAGGCTTTGCTGCGCTGCATGAAAAA
GTGCGTGCCGAACTGAACGACACGGTGGTGGATATTGCGAAGCAGGTCGAGCAAATCCTT
ACGGCAGTGTTCAATATCAACAAACGTCTGAAAGGGCGGGTGGATATGACCATGGCGCTG
GGGCTTTCTGACATTAAAGCGCAGATGGGCGGGTTGGTATATCGCGGTTTTGTCACTGGT
AACGGCTTCAAACGGCTGGGCGACACGCTGCGATATTTGCAGGCGATTGAAAAACGGCTG
GAAAAACTGGCGGTTGATCCACATCGCGACCGTGCGCAGATGCTGAAAGTCGAAAACGTC
CAGCAGGCGTGGCAGCAATGGATCAACAAACTGCCGCCCGCACGTCGTGAGGATGAAGAC
GTGAAAGAGATCCGTTGGATGATAGAAGAGTTGCGCGTTAGTTACTTCGCTCAACAACTT
GGTACGCCTTATCCGATTTCAGATAAGCGTATTTTGCAGGCGATGGAGCAGATTAGCGGT
TAA

Protein Properties

Pfam Domain Function:

Helicase_C (PF00271 )
DEAD (PF00270 )
DUF1605 (PF07717 )
HA2 (PF04408 )

Protein Residues:

1300

Protein Molecular Weight:

149027

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>ATP-dependent RNA helicase hrpA
MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQAIFQEMAKEIDQAAGK
VLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGETGSGKTTQLPKICMELGRG
IKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRFSDHVSDNTMVKLMTDGILLA
EIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLPRRPDLKIIITSATIDPERFSRH
FNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQAIFDAVDELSQESHGDILIFMSGE
REIRDTADALNKLNLRHTEILPLYARLSNSEQNRVFQSHSGRRIVLATNVAETSLTVPGI
KYVIDPGTARISRYSYRTKVQRLPIEPISQASANQRKGRCGRVSEGICIRLYSEDDFLSR
PEFTDPEILRTNLASVILQMTALGLGDIAAFPFVEAPDKRNIQDGVRLLEELGAITTDEQ
ASAYKLTPLGRQLSQLPVDPRLARMVLEAQKHGCVREAMIITSALSIQDPRERPMDKQQA
SDEKHRRFHDKESDFLAFVNLWNYLGEQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQ
LRQVVKELGIPVNSEPAEYREIHIALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGL
FKKPPKWVMVAELVETSRLWGRIAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATE
KVTVYGLPIVAARKVNYSQIDPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELE
HKSRRRDILVDDETLFEFYDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGA
EKISKLDYPNFWHQGNLKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRR
ELVIALIKSLPKPVRRNFVPAPNYAEAFLGRVKPLELPLLDSLERELRRMTGVTVDREDW
HWDQVPDHLKITFRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIW
SFGQLPESYEQKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPS
PIKYLHEKLPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEK
VRAELNDTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTG
NGFKRLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDED
VKEIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG

References

External Links:

Resource	Link
Uniprot ID:	P43329
Uniprot Name:	HRPA_ECOLI
GenBank Gene ID:	U00096
Genebank Protein ID:	145693127
CCDB:	HRPA_ECOLI

General Reference:

Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kasai, H., Kashimoto, K., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Horiuchi, T., et, a. l. .. (1996). "A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." DNA Res 3:363-377. Pubmed: 9097039
Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Moriya, H., Kasai, H., Isono, K. (1995). "Cloning and characterization of the hrpA gene in the terC region of Escherichia coli that is highly similar to the DEAH family RNA helicase genes of Saccharomyces cerevisiae." Nucleic Acids Res 23:595-598. Pubmed: 7899078

ATP-dependent RNA helicase hrpA (P43329)