Sensor protein torS (P39453)

Identification
Name:Sensor protein torS
Synonyms:Not Available
Gene Name:torS
Enzyme Class:
Biological Properties
General Function:Signal transduction mechanisms
Specific Function:Member of the two-component regulatory system torS/torR involved in the anaerobic utilization of trimethylamine-N-oxide (TMAO). Detects the presence of TMAO in the medium and, in response, activates torR via a four-step phosphorelay. When TMAO is removed, torS can dephosphorylate torR, probably by a reverse phosphorelay involving His-860 and Asp-733
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:
Metabolites:
ECMDB IDNameView
ECMDB00538Adenosine triphosphateMetaboCard
ECMDB01341ADPMetaboCard
GO Classification:
Component
cell part
integral to membrane
intrinsic to membrane
membrane
membrane part
Function
adenyl nucleotide binding
adenyl ribonucleotide binding
ATP binding
binding
catalytic activity
kinase activity
molecular transducer activity
nucleoside binding
protein histidine kinase activity
protein kinase activity
purine nucleoside binding
signal transducer activity
transferase activity
transferase activity, transferring phosphorus-containing groups
two-component response regulator activity
two-component sensor activity
Process
biological regulation
cellular metabolic process
metabolic process
peptidyl-histidine phosphorylation
phosphate metabolic process
phosphorus metabolic process
phosphorylation
protein amino acid phosphorylation
regulation of biological process
regulation of cellular process
regulation of gene expression
regulation of macromolecule metabolic process
regulation of metabolic process
regulation of transcription
regulation of transcription, DNA-dependent
signal transduction
signal transmission
signaling process
two-component signal transduction system (phosphorelay)
Gene Properties
Blattner:Not Available
Gene OrientationNot Available
Centisome Percentage:Not Available
Left Sequence EndNot Available
Right Sequence EndNot Available
Gene Sequence:
>2745 bp
GTGAATTTAACCCTGACCCGAAGACTCTGGATGGGCTTTGCCCTGATGGCGCTGTTAACC
CTGACCAGTACCCTGGTGGGATGGTACAACCTGCGCTTTATCAGCCAGGTGGAAAAAGAC
AACACTCAGGCATTGATTCCTACCATGAATATGGCGCGCCAGTTGAGCGAAGCCAGCGCC
TGGGAACTTTTCGCCGCGCAGAACCTGACCAGTGCCGATAACGAAAAGATGTGGCAGGCG
CAGGGGCGAATGCTCACCGCACAAAGCCTGAAGATTAATGCGTTGCTGCAAGCGTTACGG
GAACAAGGTTTTGATACCACCGCTATTGAACAACAGGAGCAGGAGATCTCCCGTTCGTTA
CGTCAGCAAGGGGAACTGGTGGGGCAGCGTTTGCAACTGCGCCAGCAACAACAGCAACTC
AGTCAGCAGATAGTCGCTGCCGCCGATGAGATCGCACGCCTGGCGCAAGGTCAGGCGAAT
AATGCGACAACTTCCGCTGGAGCGACCCAGGCCGGGATTTACGATTTGATCGAACAAGAT
CAGCGTCAGGCTGCTGAAAGTGCACTCGATCGGCTGATTGATATCGATCTTGAGTATGTT
AACCAGATGAATGAACTGCGCCTTAGCGCTCTGCGGGTGCAGCAAATGGTGATGAATCTG
GGGCTGGAGCAGATCCAGAAAAATGCACCAACGCTGGAAAAGCAGCTCAATAATGCGGTG
AAAATTCTGCAACGTCGGCAAATACGCATTGAAGATCCGGGTGTTCGTGCGCAGGTCGCA
ACAACGTTAACTACCGTTAGCCAATATAGCGATTTGCTGGCGCTGTATCAGCAGGACAGT
GAAATCAGCAATCACCTACAAACTCTCGCACAAAATAACATCGCCCAGTTCGCGCAGTTT
AGTAGCGAAGTCAGTCAGCTGGTCGACACCATTGAGCTGCGTAATCAGCACGGACTGGCG
CATCTGGAAAAAGCGAGTGCACGCGGGCAATACAGCCTGTTATTGCTGGGGATGGTTTCA
CTTTGCGCACTGATTCTGATCCTCTGGCGCGTGGTTTATCGCTCAGTCACGCGTCCACTT
GCCGAACAAACGCAGGCGCTGCAACGGCTGCTGGACGGTGATATTGACTCCCCTTTCCCG
GAAACCGCTGGCGTACGGGAGCTGGATACCATCGGGCGGCTGATGGATGCGTTTCGCAGC
AATGTTCATGCACTGAATCGCCACCGCGAACAGCTGGCGGCGCAGGTCAAAGCGCGTACA
GCTGAATTGCAGGAACTGGTGATAGAACACCGACAGGCACGGGCGGAAGCAGAAAAAGCC
AGCCAGGCAAAATCGGCGTTTCTGGCGGCGATGAGCCATGAGATCCGCACACCGCTGTAC
GGTATTCTCGGCACTGCTCAACTGCTGGCAGATAACCCCGCACTTAACGCCCAGCGTGAT
GATTTGCGGGCAATTACTGACTCTGGTGAATCGTTGCTGACCATCCTCAACGATATTCTC
GATTATTCCGCTATCGAAGCAGGTGGCAAGAATGTTTCGGTCAGCGATGAGCCCTTTGAA
CCGCGCCCGCTGCTGGAAAGTACCCTGCAATTAATGAGCGGACGGGTGAAAGGTCGCCCG
ATTCGCCTGGCAACGGCAATTGCCGATGATATGCCGTGCGCGTTAATGGGCGATCCACGA
CGTATTCGTCAGGTTATAACCAACCTGTTGAGCAACGCCCTGCGTTTTACTGACGAAGGG
TACATTATCCTGCGTAGTCGCACTGATGGCGAGCAATGGCTGGTCGAAGTGGAAGACAGC
GGCTGCGGTATTGATCCCGCGAAACTGGCAGAAATCTTCCAGCCATTTGTGCAGGTAAGC
GGCAAACGCGGCGGCACCGGGCTGGGACTGACTATCAGTAGCCGTCTGGCCCAGGCGATG
GGCGGCGAACTGAGCGCCACCAGCACGCCGGAGGTTGGAAGCTGTTTTTGTTTACGCTTG
CCGTTACGTGTTGCCACGGCACCCGTGCCAAAAACAGTCAATCAGGCGGTGCGTCTTGAC
GGTTTACGTTTGCTGTTAATTGAAGATAACCCGCTAACCCAGCGAATTACCATTGAGATG
CTGAAAACCAGTGGTGCGCAGATTGTTGCTGTTGGCAATGCCGCGCAGGCTTTAGAGACA
CTGCAAAATAGCGAACCGTTTGCTGCCGCACTGGTGGATTTTGATCTGCCGGATATCGAC
GGCATTACGCTTGCCCGACAACTGGCACAGCAATATCCGTCGCTGGTTTTGATTGGCTTT
AGCGCCCATGTCATTGACGAAACGCTGCGCCAGCGTACCAGTTCATTATTCCGCGGGATT
ATCCCTAAACCGGTGCCGCGTGAAGTGCTCGGTCAGTTACTGGCGCACTATCTCCAACTG
CAAGTCAATAATGATCAATCGCTGGATGTATCGCAACTCAATGAAGATGCTCAGTTAATG
GGGACGGAGAAGATCCACGAATGGCTGGTATTATTTACACAACATGCCCTGCCGCTTCTC
GATGAAATCGACATTGCCCGCGCCAGTCAGGATAGCGAGAAAATAAAGCGTGCCGCGCAT
CAGCTAAAAAGCAGTTGCTCGAGTCTGGGAATGCATATCGCCAGCCAGCTATGCGCACAA
CTGGAGCAGCAGCCATTATCTGCCCCCCTTCCACACGAAGAAATTACACGCAGTGTTGCC
GCTCTGGAAGCATGGTTGCATAAGAAAGACCTGAACGCGATTTGA
Protein Properties
Pfam Domain Function:
Protein Residues:914
Protein Molecular Weight:101023
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • 9-29
  • 333-353
Protein Sequence:
>Sensor protein torS
MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQALIPTMNMARQLSEASA
WELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQGFDTTAIEQQEQEISRSL
RQQGELVGQRLQLRQQQQQLSQQIVAAADEIARLAQGQANNATTSAGATQAGIYDLIEQD
QRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVMNLGLEQIQKNAPTLEKQLNNAV
KILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALYQQDSEISNHLQTLAQNNIAQFAQF
SSEVSQLVDTIELRNQHGLAHLEKASARGQYSLLLLGMVSLCALILILWRVVYRSVTRPL
AEQTQALQRLLDGDIDSPFPETAGVRELDTIGRLMDAFRSNVHALNRHREQLAAQVKART
AELQELVIEHRQARAEAEKASQAKSAFLAAMSHEIRTPLYGILGTAQLLADNPALNAQRD
DLRAITDSGESLLTILNDILDYSAIEAGGKNVSVSDEPFEPRPLLESTLQLMSGRVKGRP
IRLATAIADDMPCALMGDPRRIRQVITNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDS
GCGIDPAKLAEIFQPFVQVSGKRGGTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRL
PLRVATAPVPKTVNQAVRLDGLRLLLIEDNPLTQRITIEMLKTSGAQIVAVGNAAQALET
LQNSEPFAAALVDFDLPDIDGITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGI
IPKPVPREVLGQLLAHYLQLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLL
DEIDIARASQDSEKIKRAAHQLKSSCSSLGMHIASQLCAQLEQQPLSAPLPHEEITRSVA
ALEAWLHKKDLNAI
References
External Links:
ResourceLink
Uniprot ID:P39453
Uniprot Name:TORS_ECOLI
GenBank Gene ID:U00096
Genebank Protein ID:87081816
CCDB:TORS_ECOLI
General Reference:
  • Ansaldi, M., Jourlin-Castelli, C., Lepelletier, M., Theraulaz, L., Mejean, V. (2001). "Rapid dephosphorylation of the TorR response regulator by the TorS unorthodox sensor in Escherichia coli." J Bacteriol 183:2691-2695. Pubmed: 11274133
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Jourlin, C., Ansaldi, M., Mejean, V. (1997). "Transphosphorylation of the TorR response regulator requires the three phosphorylation sites of the TorS unorthodox sensor in Escherichia coli." J Mol Biol 267:770-777. Pubmed: 9135110
  • Jourlin, C., Bengrine, A., Chippaux, M., Mejean, V. (1996). "An unorthodox sensor protein (TorS) mediates the induction of the tor structural genes in response to trimethylamine N-oxide in Escherichia coli." Mol Microbiol 20:1297-1306. Pubmed: 8809780
  • Oshima, T., Aiba, H., Baba, T., Fujita, K., Hayashi, K., Honjo, A., Ikemoto, K., Inada, T., Itoh, T., Kajihara, M., Kanai, K., Kashimoto, K., Kimura, S., Kitagawa, M., Makino, K., Masuda, S., Miki, T., Mizobuchi, K., Mori, H., Motomura, K., Nakamura, Y., Nashimoto, H., Nishio, Y., Saito, N., Horiuchi, T., et, a. l. .. (1996). "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 12.7-28.0 min region on the linkage map." DNA Res 3:137-155. Pubmed: 8905232
  • Simon, G., Mejean, V., Jourlin, C., Chippaux, M., Pascal, M. C. (1994). "The torR gene of Escherichia coli encodes a response regulator protein involved in the expression of the trimethylamine N-oxide reductase genes." J Bacteriol 176:5601-5606. Pubmed: 8083154