Probable L-ascorbate-6-phosphate lactonase ulaG (P39300)

Identification
Name:Probable L-ascorbate-6-phosphate lactonase ulaG
Synonyms:
  • L-ascorbate utilization protein G
Gene Name:ulaG
Enzyme Class:Not Available
Biological Properties
General Function:Involved in hydrolase activity
Specific Function:Probably catalyzes the hydrolysis of L-ascorbate-6-P into 3-keto-L-gulonate-6-P. Is essential for L-ascorbate utilization under anaerobic conditions. Also shows phosphodiesterase activity, hydrolyzing phosphodiester bond in the artificial chromogenic substrate bis-p-nitrophenyl phosphate (bis- pNPP)
Cellular Location:Cytoplasm (Probable)
SMPDB Pathways:
KEGG Pathways:
  • Ascorbate and aldarate metabolism ec00053
KEGG Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0L-Ascorbate 6-phosphate + 1.0Water → 1.03-Dehydro-L-gulonate 6-phosphate + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb
1.0L-Ascorbate 6-phosphate + 1.0Water ↔ 1.03-Dehydro-L-gulonate 6-phosphate
ReactionCard
SMPDB Reactions:
1.0L-ascorbate 6-phosphate+1.0Thumb+1.0Thumb → 1.03-keto-L-gulonate 6-phosphate+1.0Thumb
1.0L-ascorbate 6-phosphate + 1.0Water + 1.0L-Ascorbate 6-phosphate → 1.03-keto-L-gulonate 6-phosphate + 1.03-Keto-L-gulonate 6-phosphate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0L-Ascorbate 6-phosphate + 1.0Water → 1.03-Dehydro-L-gulonate 6-phosphate + 1.0Hydrogen ion
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb
1.0L-Ascorbate 6-phosphate + 1.0Water ↔ 1.03-Dehydro-L-gulonate 6-phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB200673-Dehydro-L-gulonate 6-phosphateMetaboCard
ECMDB202713-Keto-L-gulonate 6-phosphateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB20164L-Ascorbate 6-phosphateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
catalytic activity
hydrolase activity
Gene Properties
Blattner:b4192
Gene OrientationCounterclockwise
Centisome Percentage:95.19
Left Sequence End4416584
Right Sequence End4417648
Gene Sequence:
>1065 bp
ATGGTAACGTCCTGTACTGGACATGTTTTAGATAATCAACGCGCCACCACTCGCGGAGTT
TTCTCCTCCGGTAGTCATCTCGTCACTTTGCATTTTCAACCTCATCCTTTCTTTTCATGT
GTTACCGACGCCGTAAACGGTGCGCGTAGCCGTTTTTCGGCATTTTATCCAAAAGCAAAC
TATGGCTTACAAGGAAGCCAACCCTCTGATGTTCGTGCGCATAATCGCGCTGCCAACGGC
GCGTGTGATGAATACAAACAACTCAAGGTGCTCTCCATGGGAAGACAAAAAGCAGTGATC
AAAGCTCGTCGCGAGGCAAAACGTGTGCTGAGACGGGATTCGCGCAGCCATAAGCAGCGT
GAAGAAGAATCGGTCACCTCGCTTGTGCAGATGGGCGGCGTAGAAGCCATTGGTATGGCC
CGCGACAGTCGCGATACTTCGCCCATCCTCGCGCGAAATGAAGCGCAATTGCACTATCTG
AAGGCTATTGAGAGTAAGCAGCTGATATTCGCCACGGGCGAAGCCGGGTGCGGAAAAACC
TGGATCAGCGCAGCAAAAGCGGCAGAGGCCCTGATACATAAGGATGTCGACAGGATTATC
GTCACCCGTCCAGTTCTGCAAGCCGATGAAGATCTTGGCTTCTTACCTGGAGATATCGCA
GAAAAGTTTGCTCCCTATTTTCGCCCGGTCTACGACGTGCTGGTCCGGCGCTTAGGGGCT
TCCTTTATGCAGTACTGCCTGCGACCGGAAATAGGGAAGGTGGAAATTGCGCCGTTCGCC
TATATGCGTGGACGTACCTTTGAAAATGCAGTCGTCATTCTTGACGAGGCGCAGAATGTG
ACTGCCGCGCAAATGAAAATGTTTTTAACCCGCCTCGGGGAGAACGTGACGGTTATCGTC
AACGGTGATATCACGCAATGCGATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCG
CTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGC
GTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAA
Protein Properties
Pfam Domain Function:Not Available
Protein Residues:354
Protein Molecular Weight:40060
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Probable L-ascorbate-6-phosphate lactonase ulaG
MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLGCTGIWLKSEGGTNVCV
DFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVLDPFAIRQIDAVLATHDHN
DHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKERCIVVKPGDVVKVKDIEIHAL
DAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPGGSLYHSGDSHYSNYYAKHGNEH
QIDVALGSYGENPRGITDKMTSADMLRMGEALNAKVVIPFHHDIWSNFQADPQEIRVLWE
MKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHYPRGFDDCFTIEPDLPFKSFL
References
External Links:
ResourceLink
Uniprot ID:P39300
Uniprot Name:ULAG_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1651507
Ecogene ID:EG12492
Ecocyc:EG12492
ColiBase:b4192
Kegg Gene:b4192
EchoBASE ID:EB2385
CCDB:ULAG_ECOLI
BacMap:90111702
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L., Blattner, F. R. (1995). "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Nucleic Acids Res 23:2105-2119. Pubmed: 7610040
  • Campos, E., Aguilar, J., Baldoma, L., Badia, J. (2002). "The gene yjfQ encodes the repressor of the yjfR-X regulon (ula), which is involved in L-ascorbate metabolism in Escherichia coli." J Bacteriol 184:6065-6068. Pubmed: 12374842
  • Campos, E., Baldoma, L., Aguilar, J., Badia, J. (2004). "Regulation of expression of the divergent ulaG and ulaABCDEF operons involved in LaAscorbate dissimilation in Escherichia coli." J Bacteriol 186:1720-1728. Pubmed: 14996803
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Kuznetsova, E., Proudfoot, M., Sanders, S. A., Reinking, J., Savchenko, A., Arrowsmith, C. H., Edwards, A. M., Yakunin, A. F. (2005). "Enzyme genomics: Application of general enzymatic screens to discover new enzymes." FEMS Microbiol Rev 29:263-279. Pubmed: 15808744
  • Zhang, Z., Aboulwafa, M., Smith, M. H., Saier, M. H. Jr (2003). "The ascorbate transporter of Escherichia coli." J Bacteriol 185:2243-2250. Pubmed: 12644495