Menaquinone-specific isochorismate synthase (P38051)

Identification
Name:Menaquinone-specific isochorismate synthase
Synonyms:
  • Isochorismate mutase
Gene Name:menF
Enzyme Class:
Biological Properties
General Function:Involved in biosynthetic process
Specific Function:Chorismate = isochorismate
Cellular Location:Not Available
SMPDB Pathways:
  • Biosynthesis of siderophore group nonribosomal peptides PW000760
KEGG Pathways:
  • Biosynthesis of siderophore group nonribosomal peptides ec01053
  • Metabolic pathways eco01100
  • Ubiquinone and other terpenoid-quinone biosynthesis ec00130
KEGG Reactions:
1.0Thumb ↔ 1.0Thumb
1.0Chorismate ↔ 1.0Isochorismate
ReactionCard
EcoCyc Reactions:
1.0Thumb ↔ 1.0Thumb
1.0Chorismate ↔ 1.0Isochorismate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB12199ChorismateMetaboCard
ECMDB20162IsochorismateMetaboCard
GO Classification:
Function
catalytic activity
intramolecular transferase activity
intramolecular transferase activity, transferring hydroxy groups
isochorismate synthase activity
isomerase activity
Process
biosynthetic process
metabolic process
Gene Properties
Blattner:b2265
Gene OrientationCounterclockwise
Centisome Percentage:51.24
Left Sequence End2377370
Right Sequence End2378665
Gene Sequence:
>1296 bp
GTGCTGGAACGGCTGTCGTGGAAAAGGCTGGTGCTGGAGCTGCTACTTTGCTGCCTCCCG
GCTTTCATCCTGGGTGCATTTTTTGGTTACCTGCCCTGGTTTTTGCTGGCATCGGTAACA
GGACTGCTTATCTGGCATTTCTGGAATTTATTGCGCCTTTCATGGTGGCTGTGGGTGGAT
CGCAGTATGACCCCGCCACCGGGGCGTGGTAGCTGGGAACCGCTACTATACGGCTTACAC
CAGATGCAGCTGCGAAATAAAAAACGCCGCCGTGAACTGGGCAATCTGATTAAACGCTTT
CGTAGCGGCGCGGAGTCGCTGCCCGACGCGGTGGTGCTGACCACGGAAGAGGGCGGTATT
TTCTGGTGTAACGGTCTGGCGCAACAAATTCTTGGTTTGCGCTGGCCGGAAGATAACGGG
CAGAACATCCTTAACCTACTGCGTTACCCGGAGTTTACGCAATATCTGAAAACGCGTGAT
TTTTCTCGCCCGCTCAATCTGGTGCTCAACACCGGGCGGCATCTGGAAATTCGCGTCATG
CCTTATACCCACAAACAGTTGCTGATGGTGGCGCGTGATGTCACGCAAATGCATCAACTG
GAAGGGGCGCGGCGTAACTTTTTTGCCAACGTGAGCCATGAGTTACGTACGCCATTGACC
GTGTTACAGGGTTACCTGGAGATGATGAATGAGCAGCCGCTGGAAGGCGCGGTACGCGAA
AAAGCGTTGCACACCATGCGCGAGCAGACCCAGCGGATGGAAGGACTGGTGAAGCAATTG
CTGACGCTGTCGAAAATAGAAGCCGCACCGACGCATTTGCTCAATGAAAAGGTTGATGTG
CCGATGATGCTGCGCGTTGTTGAGCGCGAGGCTCAGACTCTGAGTCAGAAAAAACAGACA
TTTACCTTTGAGATAGATAACGGCCTCAAGGTGTCTGGCAACGAAGATCAGCTACGCAGT
GCGATTTCGAACCTGGTCTATAACGCCGTGAATCATACGCCGGAAGGCACGCATATCACC
GTACGCTGGCAGCGAGTGCCGCACGGTGCCGAATTTAGCGTTGAAGATAACGGACCGGGC
ATTGCACCGGAGCATATTCCGCGCCTGACCGAGCGTTTTTATCGCGTTGATAAAGCGCGT
TCCCGGCAAACCGGCGGTAGCGGATTAGGGTTAGCGATCGTGAAACATGCTGTGAATCAT
CACGAAAGTCGCCTGAATATTGAGAGTACAGTAGGAAAAGGAACACGTTTCAGTTTTGTT
ATCCCGGAACGTTTAATTGCCAAAAACAGCGATTAA
Protein Properties
Pfam Domain Function:
Protein Residues:431
Protein Molecular Weight:48764
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Menaquinone-specific isochorismate synthase
MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWLASQQTYPQFYWQQRNG
DEEAVVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDPSQGNLLLPRLEWRRCGGK
ATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTTTREQHWPDKTGWTQLIELAT
KTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLNLNCYHFYMAFDGENAFLGSSPE
RLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLMADDKNQRENMLVVEDICQRLQADT
QTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVICLHQLQPTAAVAGLPRDLARQFIARH
EPFTREWYAGSAGYLSLQQSEFCVSLRSAKISGNVVRLYAGAGIVRGSDPEQEWQEIDNK
AAGLRTLLQME
References
External Links:
ResourceLink
Uniprot ID:P38051
Uniprot Name:MENF_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674540
Ecogene ID:EG12362
Ecocyc:EG12362
ColiBase:b2265
Kegg Gene:b2265
EchoBASE ID:EB2265
CCDB:MENF_ECOLI
BacMap:90111411
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Borodovsky, M., Rudd, K. E., Koonin, E. V. (1994). "Intrinsic and extrinsic approaches for detecting genes in a bacterial genome." Nucleic Acids Res 22:4756-4767. Pubmed: 7984428
  • Daruwala, R., Kwon, O., Meganathan, R., Hudspeth, M. E. (1996). "A new isochorismate synthase specifically involved in menaquinone (vitamin K2) biosynthesis encoded by the menF gene." FEMS Microbiol Lett 140:159-163. Pubmed: 8764478
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Muller, R., Dahm, C., Schulte, G., Leistner, E. (1996). "An isochorismate hydroxymutase isogene in Escherichia coli." FEBS Lett 378:131-134. Pubmed: 8549818
  • Popp, J. L. (1989). "Sequence and overexpression of the menD gene from Escherichia coli." J Bacteriol 171:4349-4354. Pubmed: 2666397
  • Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837