Identification
Name:O-antigen polymerase
Synonyms:Not Available
Gene Name:rfc
Enzyme Class:Not Available
Biological Properties
General Function:Involved in lipopolysaccharide biosynthetic process
Specific Function:May link the O-antigen tetrasaccharide units into long chains, giving rise to typical smooth LPS
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Complex Reactions:
1.0(O16 antigen)x2 undecaprenyl diphosphate+1.0Thumb1.0Thumb+1.0(O16 antigen)x3 undecaprenyl diphosphate+1.0Thumb
1.0(O16 antigen)x2 undecaprenyl diphosphate + 1.0O16 antigen undecaprenyl diphosphate → 1.0Hydrogen ion + 1.0(O16 antigen)x3 undecaprenyl diphosphate + 1.0Undecaprenyl diphosphate
ReactionCard
1.0(O16 antigen)x3 undecaprenyl diphosphate+1.0Thumb1.0Thumb+1.0(O16 antigen)x4 undecaprenyl diphosphate+1.0Thumb
1.0(O16 antigen)x3 undecaprenyl diphosphate + 1.0O16 antigen undecaprenyl diphosphate → 1.0Hydrogen ion + 1.0(O16 antigen)x4 undecaprenyl diphosphate + 1.0Undecaprenyl diphosphate
ReactionCard
2.0Thumb1.0Thumb+1.0(O16 antigen)x2 undecaprenyl diphosphate+1.0Thumb
2.0O16 antigen undecaprenyl diphosphate → 1.0Hydrogen ion + 1.0(O16 antigen)x2 undecaprenyl diphosphate + 1.0Undecaprenyl diphosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB21225Hydrogen ionMetaboCard
ECMDB21257O16 antigen undecaprenyl diphosphateMetaboCard
ECMDB01469Undecaprenyl diphosphateMetaboCard
GO Classification:Not Available
Gene Properties
Blattner:b2035
Gene OrientationCounterclockwise
Centisome Percentage:45.35
Left Sequence End2104084
Right Sequence End2105250
Gene Sequence:
>1167 bp
GTGAAACATCTGCATCGATTCTTTAGCAGTGATGCCTCGGGAGGCATTATTCTTATCATT
GCCGCTATCCTGGCGATGATTATGGCCAACAGCGGCGCAACCAGTGGATGGTATCACGAC
TTTCTGGAGACGCCGGTTCAGCTCCGGGTTGGTTCACTCGAAATCAACAAAAACATGCTG
TTATGGATAAATGACGCGCTGATGGCGGTATTTTTCCTGTTAGTCGGTCTGGAAGTTAAA
CGTGAACTGATGCAAGGATCGCTAGCCAGCTTACGCCAGGCCGCATTTCCAGTTATCGCC
GCTATTGGTGGGATGATTGTGCCGGCATTACTCTATCTGGCTTTTAACTATGCCGATCCG
ATTACCCGCGAAGGGTGGGCGATCCCGGCGGCTACTGACATTGCTTTTGCACTTGGTGTA
CTGGCGCTGTTGGGAAGTCGTGTTCCGTTAGCGCTGAAGATCTTTTTGATGGCTCTGGCT
ATTATCGACGATCTTGGGGCCATCATTATCATCGCATTGTTCTACACTAATGACTTATCG
ATGGCCTCTCTTGGCGTCGCGGCTGTAGCAATTGCGGTACTCGCGGTATTGAATCTGTGT
GGTGCACGCCGCACGGGCGTCTATATTCTTGTTGGCGTGGTGTTGTGGACTGCGGTGTTG
AAATCGGGGGTTCACGCAACTCTGGCGGGGGTAATTGTCGGCTTCTTTATTCCTTTGAAA
GAGAAGCATGGGCGTTCTCCAGCGAAGCGACTGGAGCATGTGTTGCACCCGTGGGTGGCG
TATCTGATTTTGCCGCTGTTTGCATTTGCTAATGCTGGCGTTTCACTGCAAGGCGTCACG
CTGGATGGCTTGACCTCCATTCTGCCATTGGGGATCATCGCTGGCTTGCTGATTGGCAAA
CCGCTGGGGATTAGTCTGTTCTGCTGGTTGGCGCTGCGTTTGAAACTGGCGCATCTGCCT
GAGGGAACGACTTATCAGCAAATTATGGTGGTGGGGATCCTGTGCGGTATCGGTTTTACT
ATGTCTATCTTTATTGCCAGCCTGGCCTTTGGTAGCGTAGATCCAGAACTGATTAACTGG
GCGAAACTCGGTATCCTGGTCGGTTCTATCTCTTCGGCGGTAATTGGATACAGCTGGTTA
CGCGTTCGTTTGCGTCCATCAGTTTGA
Protein Properties
Pfam Domain Function:Not Available
Protein Residues:388
Protein Molecular Weight:44744
Protein Theoretical pI:9
Signaling Regions:
  • None
Transmembrane Regions:
  • 23-43
  • 57-77
  • 97-117
  • 143-163
  • 180-200
  • 215-235
  • 312-332
  • 338-358
  • 361-381
Protein Sequence:
>O-antigen polymerase
MIYLVISVFLITAFICLYLKKDIFYPAVCVNIIFALVLLGYEITSDIYAFQLNDATLIFL
LCNVLTFTLSCLLTESVLDLNIRKVNNAIYSIPSKKVHNVGLLVISFSMIYICMRLSNYQ
FGTSLLSYMNLIRDADVEDTSRNFSAYMQPIILTTFALFIWSKKFTNTKVSKTFTLLVFI
VFIFAIILNTGKQIVFMVIISYAFIVGVNRVKHYVYLITAVGVLFSLYMLFLRGLPGGMA
YYLSMYLVSPIIAFQEFYFQQVSNSASSHVFWFFERLMGLLTGGVSMSLHKEFVWVGLPT
NVYTAFSDYVYISAELSYLMMVIHGCISGVLWRLSRNYISVKIFYSYFIYTFSFIFYHES
FMTNISSWIQITLCIIVFSQFLKAQKIK
References
External Links:
ResourceLink
Uniprot ID:P37748
Uniprot Name:RFC_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:21321905
Ecogene ID:EG11982
Ecocyc:EG11982
ColiBase:b2035
Kegg Gene:b2035
EchoBASE ID:EB1925
CCDB:RFC_ECOLI
BacMap:16129975
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Itoh, T., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Kasai, H., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Seki, Y., Horiuchi, T., et, a. l. .. (1996). "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." DNA Res 3:379-392. Pubmed: 9097040
  • Stevenson, G., Neal, B., Liu, D., Hobbs, M., Packer, N. H., Batley, M., Redmond, J. W., Lindquist, L., Reeves, P. (1994). "Structure of the O antigen of Escherichia coli K-12 and the sequence of its rfb gene cluster." J Bacteriol 176:4144-4156. Pubmed: 7517391
  • Yao, Z., Valvano, M. A. (1994). "Genetic analysis of the O-specific lipopolysaccharide biosynthesis region (rfb) of Escherichia coli K-12 W3110: identification of genes that confer group 6 specificity to Shigella flexneri serotypes Y and 4a." J Bacteriol 176:4133-4143. Pubmed: 7517390