2,3-diketo-L-gulonate-binding periplasmic protein yiaO (P37676)

Identification
Name:2,3-diketo-L-gulonate-binding periplasmic protein yiaO
Synonyms:
  • 2,3-DKG-binding protein
  • Extracytoplasmic solute receptor protein yiaO
Gene Name:yiaO
Enzyme Class:Not Available
Biological Properties
General Function:Involved in transport
Specific Function:Part of the tripartite ATP-independent periplasmic (TRAP) transport system yiaMNO involved in the uptake of 2,3- diketo-L-gulonate. This protein specifically binds 2,3-diketo-L- gulonate. Is not able to bind either L-ascorbate or dehydroascorbate
Cellular Location:Periplasm
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Transports:
Transport References:
  • Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
Metabolites:
ECMDB IDNameView
ECMDB21225Hydrogen ionMetaboCard
ECMDB00751L-Threo-2-pentuloseMetaboCard
GO Classification:
Component
cell part
outer membrane-bounded periplasmic space
periplasmic space
Process
establishment of localization
transport
Gene Properties
Blattner:b3579
Gene OrientationClockwise
Centisome Percentage:80.70
Left Sequence End3744117
Right Sequence End3745103
Gene Sequence:
>987 bp
ATGATCGAAAAAATCTGGTCTGGTGAATCCCCTTTGTGGCGGCTATTGCTGCCACTCTCC
TGGTTGTATGGCCTGGTGAGTGGCGCGATCCGTCTTTGCTATAAACTAAAACTGAAGCGC
GCCTGGCGTGCCCCCGTACCGGTTGTCGTGGTTGGTAATCTCACCGCAGGCGGCAACGGA
AAAACCCCGGTCGTTGTCTGGCTGGTGGAACAGTTGCAACAGCGCGGTATTCGCGTGGGG
GTCGTATCGCGGGGATATGGTGGTAAGGCTGAATCTTATCCGCTGTTATTGTCGGCAGAT
ACCACAACAGCACAGGCGGGTGATGAACCTGTGTTGATTTATCAACGCACTGATGCGCCT
GTTGCGGTTTCTCCCGTTCGTTCTGATGCGGTAAAAGCCATTCTGGCGCAACACCCTGAT
GTGCAGATCATCGTAACCGACGACGGTTTACAGCATTACCGTCTGGCGCGTGATGTGGAA
ATTGTCGTTATTGATGGTGTGCGTCGCTTTGGCAATGGCTGGTGGTTGCCGGCGGGGCCA
ATGCGTGAGCGAGCGGGGCGCTTAAAGTCGGTTGATGCGGTAATCGTCAACGGCGGTGTC
CCTCGCAGCGGTGAAATCCCCATGCATCTGCTGCCGGGTCAGGCGGTGAATTTACGTACC
GGTACGCGTTGTGACGTTGCTCAGCTTGAACATGTAGTGGCGATGGCGGGGATTGGGCAT
CCGCCGCGCTTTTTTGCCACGCTGAAGATGTGTGGCGTACAACCGGAAAAATGTGTACCG
CTGGCCGATCATCAGTCTTTGAACCATGCGGATGTCAGTGCGTTGGTAAGCGCCGGGCAA
ACGCTGGTAATGACTGAAAAAGATGCGGTGAAATGCCGGGCCTTTGCAGAAGAAAATTGG
TGGTATTTGCCTGTAGACGCACAGCTTTCAGGTGATGAACCAGCGAAACTGCTTACGCAA
CTAACCTTGCTGGCTTCTGGCAACTAG
Protein Properties
Pfam Domain Function:
Protein Residues:328
Protein Molecular Weight:35970
Protein Theoretical pI:7
Signaling Regions:
  • 1-24
Transmembrane Regions:
  • None
Protein Sequence:
>2,3-diketo-L-gulonate-binding periplasmic protein yiaO
MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAAKKFNDLLQERTKGELK
LKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMNLLDVPFLFRDTAHAHKTL
DGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTPADLKGLKIRTNNSPMNIAAF
KVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAKFFEVQKFLSLTHHAYSPLLVVI
NKAKFDGLSPEFQQALVSSAQEAGNYQRKLVAEDQQKIIDGMKEAGVEVITDLDRKAFSD
ALGNQVRDMFVKDVPQGADLLKAVDEVQ
References
External Links:
ResourceLink
Uniprot ID:P37676
Uniprot Name:YIAO_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4062487
Ecogene ID:EG12283
Ecocyc:EG12283
ColiBase:b3579
Kegg Gene:b3579
EchoBASE ID:EB2191
CCDB:YIAO_ECOLI
BacMap:16131450
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Plantinga, T. H., Van Der Does, C., Badia, J., Aguilar, J., Konings, W. N., Driessen, A. J. (2004). "Functional characterization of the Escherichia coli K-12 yiaMNO transport protein genes." Mol Membr Biol 21:51-57. Pubmed: 14668138
  • Plantinga, T. H., van der Does, C., Tomkiewicz, D., van Keulen, G., Konings, W. N., Driessen, A. J. (2005). "Deletion of the yiaMNO transporter genes affects the growth characteristics of Escherichia coli K-12." Microbiology 151:1683-1689. Pubmed: 15870475
  • Sofia, H. J., Burland, V., Daniels, D. L., Plunkett, G. 3rd, Blattner, F. R. (1994). "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Nucleic Acids Res 22:2576-2586. Pubmed: 8041620
  • Thomas, G. H., Southworth, T., Leon-Kempis, M. R., Leech, A., Kelly, D. J. (2006). "Novel ligands for the extracellular solute receptors of two bacterial TRAP transporters." Microbiology 152:187-198. Pubmed: 16385129