ECMDB

Identification

Name:

2-succinylbenzoate--CoA ligase

Synonyms:

o-succinylbenzoyl-CoA synthetase
OSB-CoA synthetase

Gene Name:

menE

Enzyme Class:

6.2.1.26

Biological Properties

General Function:

Involved in catalytic activity

Specific Function:

Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA)

Cellular Location:

Not Available

SMPDB Pathways:

Menaquinol biosythesis PW001897

KEGG Pathways:

Metabolic pathways eco01100
Ubiquinone and other terpenoid-quinone biosynthesis ec00130

KEGG Reactions:

1.0

↔

1.0

1.0Adenosine triphosphate + 1.0Coenzyme A + 1.02-Succinylbenzoate ↔ 1.0Adenosine monophosphate + 1.0Pyrophosphate + 1.02-Succinylbenzoyl-CoA
ReactionCard

1.0

→

1.0

1.0Adenosine triphosphate + 1.02-Succinylbenzoate + 1.0Coenzyme A → 1.0Adenosine monophosphate + 1.0Pyrophosphate + 1.02-Succinylbenzoyl-CoA
ReactionCard

SMPDB Reactions:

1.02-succinylbenzoate

1.0

→

1.0

1.0Pyrophosphate

1.0

1.02-succinylbenzoate + 1.0Coenzyme A + 1.0Adenosine triphosphate + 1.02-Succinylbenzoate → 1.02-Succinylbenzoyl-CoA + 1.0Pyrophosphate + 1.0Adenosine monophosphate
ReactionCard

EcoCyc Reactions:

1.0

↔

1.0

1.0Adenosine triphosphate + 1.0Coenzyme A + 1.02-Succinylbenzoate ↔ 1.0Adenosine monophosphate + 1.0Pyrophosphate + 1.02-Succinylbenzoyl-CoA
ReactionCard

1.0

→

1.0

1.0Adenosine triphosphate + 1.02-Succinylbenzoate + 1.0Coenzyme A → 1.0Adenosine monophosphate + 1.0Pyrophosphate + 1.02-Succinylbenzoyl-CoA
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB20051	2-Succinylbenzoate	MetaboCard
ECMDB20052	2-Succinylbenzoyl-CoA	MetaboCard
ECMDB00045	Adenosine monophosphate	MetaboCard
ECMDB00538	Adenosine triphosphate	MetaboCard
ECMDB01423	Coenzyme A	MetaboCard
ECMDB04142	Pyrophosphate	MetaboCard

GO Classification:

Function
catalytic activity
CoA-ligase activity
ligase activity
ligase activity, forming carbon-sulfur bonds
o-succinylbenzoate-CoA ligase activity
Process
fat-soluble vitamin metabolic process
menaquinone biosynthetic process
menaquinone metabolic process
metabolic process
small molecule metabolic process
vitamin K metabolic process
vitamin metabolic process

Gene Properties

Blattner:

b2260

Gene Orientation

Counterclockwise

Centisome Percentage:

51.12

Left Sequence End

2371670

Right Sequence End

2373025

Gene Sequence:

>1356 bp
ATGATCTTCTCTGACTGGCCGTGGCGTCACTGGCGGCAAGTGCGGGGAGAAACCATCGCC
TTACGTCTTAATGACGAGCAACTCAACTGGCGCGAGCTTTGTGCTCGCGTCGATGAATTA
GCCTCCGGATTTGCGGTGCAGGGGGTGGTTGAGGGCAGCGGCGTGATGTTGCGGGCGTGG
AATACGCCGCAAACGCTGCTCGCCTGGCTGGCGTTACTGCAATGCGGGGCGCGGGTGTTG
CCCGTGAACCCTCAGCTGCCGCAACCGTTGCTTGAAGAATTGCTGCCCAATCTGACGCTG
CAATTTGCTCTGGTGCCGGATGGGGAAAACACGTTTCCGGCATTAACGTCGCTGCACATT
CAGCTGGTTGAAGGCGCACATGCCGCTACGTGGCAGCCGACGCGTCTGTGCTCAATGACG
TTGACCTCGGGTTCTACCGGTTTGCCGAAAGCCGCTGTCCATACTTATCAGGCCCATCTT
GCCAGTGCGCAAGGTGTGTTATCGCTGATTCCGTTTGGCGATCACGATGATTGGTTGCTC
TCTTTACCGCTGTTTCACGTCTCCGGTCAGGGAATTATGTGGCGCTGGTTATACGCTGGT
GCGCGGATGACGGTACGTGATAAACAGCCATTGGAGCAAATGCTGGCAGGCTGTACTCAC
GCTTCACTGGTGCCAACACAACTCTGGCGTTTGCTGGTTAACCGTAGTTCCGTTTCCCTG
AAAGCGGTGTTACTTGGCGGCGCGGCTATCCCGGTCGAGTTGACGGAACAGGCGCGCGAG
CAGGGGATTCGTTGCTTTTGCGGCTATGGTCTGACCGAGTTTGCCTCCACGGTGTGTGCG
AAAGAAGCCGACGGCCTGGCAGACGTTGGTTCGCCGCTGCCGGGTCGGGAAGTGAAAATC
GTTAATAATGAAGTGTGGCTGCGGGCTGCCAGTATGGCAGAAGGTTACTGGCGTAACGGG
CAACTGGTTTCACTGGTTAATGACGAAGGCTGGTACGCTACGCGCGATCGCGGTGAGATG
CATAATGGCAAGCTGACCATTGTCGGACGTTTAGACAATCTATTCTTCAGTGGCGGAGAG
GGTATTCAGCCGGAAGAAGTCGAGCGCGTAATTGCTGCACATCCTGCGGTTTTGCAGGTG
TTTATCGTCCCCGTTGCCGACAAGGAGTTTGGTCATCGACCGGTGGCGGTGATGGAGTAT
GACCACGAGAGCGTTGATCTTAGTGAATGGGTGAAAGATAAGCTGGCCCGTTTTCAACAA
CCGGTGCGCTGGCTAACTCTGCCGCCGGAGCTGAAAAACGGCGGTATTAAAATTTCACGT
CAGGCGCTAAAAGAGTGGGTGCAACGTCAGCAATAA

Protein Properties

Pfam Domain Function:

AMP-binding (PF00501 )

Protein Residues:

451

Protein Molecular Weight:

50184

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

None

Protein Sequence:

>2-succinylbenzoate--CoA ligase
MIFSDWPWRHWRQVRGETIALRLNDEQLNWRELCARVDELASGFAVQGVVEGSGVMLRAW
NTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQFALVPDGENTFPALTSLHI
QLVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHLASAQGVLSLIPFGDHDDWLL
SLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGCTHASLVPTQLWRLLVNRSSVSL
KAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFASTVCAKEADGLADVGSPLPGREVKI
VNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYATRDRGEMHNGKLTIVGRLDNLFFSGGE
GIQPEEVERVIAAHPAVLQVFIVPVADKEFGHRPVAVMEYDHESVDLSEWVKDKLARFQQ
PVRWLTLPPELKNGGIKISRQALKEWVQRQQ

References

External Links:

Resource	Link
Uniprot ID:	P37353
Uniprot Name:	MENE_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	1799614
Ecogene ID:	EG12437
Ecocyc:	EG12437
ColiBase:	b2260
Kegg Gene:	b2260
EchoBASE ID:	EB2332
CCDB:	MENE_ECOLI
BacMap:	16130195

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Sharma, V., Hudspeth, M. E., Meganathan, R. (1996). "Menaquinone (vitamin K2) biosynthesis: localization and characterization of the menE gene from Escherichia coli." Gene 168:43-48. Pubmed: 8626063
Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837

2-succinylbenzoate--CoA ligase (P37353)