ECMDB

Identification

Name:

L-2-hydroxyglutarate oxidase LhgO

Synonyms:

Not Available

Gene Name:

lhgO

Enzyme Class:

1.1.3.15

Biological Properties

General Function:

Not Available

Specific Function:

Oxidizes L-2-hydroxyglutarate, probably to recover alpha-ketoglutarate mistakenly reduced by other enzymes or formed during growth on propionate. Oxidizes L-2-hydroxyglutarate, probably to recover alpha-ketoglutarate mistakenly reduced by other enzymes or formed during growth on propionate.

Cellular Location:

Not Available

SMPDB Pathways:

Not Available

KEGG Pathways:

Not Available

Complex Reactions:

1.0(S)-2-hydroxy acid

1.0

→

1.02-oxo acid

1.0

1.0(S)-2-hydroxy acid + 1.0Oxygen → 1.02-oxo acid + 1.0Hydrogen peroxide
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB21232	Hydrogen peroxide	MetaboCard
ECMDB00694	L-2-Hydroxyglutaric acid	MetaboCard
ECMDB04124	Oxygen	MetaboCard

GO Classification:

Not Available

Gene Properties

Blattner:

b2660

Gene Orientation

Not Available

Centisome Percentage:

Not Available

Left Sequence End

Not Available

Right Sequence End

Not Available

Gene Sequence:

>ENA|AAC75707|AAC75707.2 Escherichia coli str. K-12 substr. MG1655 L-2-hydroxyglutarate oxidase
ATGTATGATTTTGTGATTATTGGCGGCGGCATCATCGGCATGTCGACCGCCATGCAACTG
ATTGATGTCTATCCGGACGCCCGCATTGCGTTGCTGGAAAAAGAGTCCGCCCCGGCCTGT
CACCAGACGGGCCACAACAGCGGCGTGATCCATGCCGGGGTCTATTACACGCCCGGCAGC
CTGAAGGCGCAGTTTTGCCTGGCGGGAAACCGCGCCACCAAAGCCTTTTGCGATCAAAAC
GGCATTCGCTACGACAACTGCGGCAAGATGCTGGTTGCCACGTCCGATCTCGAAATGGAA
CGGATGCGCGCCTTATGGGAACGCACAGCGGCGAACGGTATCGAGCGCGAATGGCTTAAC
GCCGACGAACTGCGCGAGCGCGAACCGAATATCACCGGGCTCGGCGGCATTTTTGTGCCG
TCCAGCGGCATTGTCAGCTACCGCGACGTGACGGCGGCGATGGCAAAAATCTTCCAGTCC
AGAGGCGGTGAAATTATCTATAACGCCGAAGTCAGCGGGCTTAATGAGCATAAAAACGGC
GTGGTGATACGTACCCGTCAGGGCGGCGAATATGAAGCATCAACGCTGATTAGCTGTTCC
GGGCTGATGGCTGACCGGCTGGTGAAAATGCTCGGACTCGAACCGGGCTTTATCATCTGC
CCGTTCCGTGGCGAGTATTTCCGCCTTGCGCCGGAGCATAACCAGATTGTTAACCACCTG
ATTTACCCCATTCCCGACCCGGCAATGCCGTTTTTGGGCGTTCATCTCACCCGCATGATC
GACGGCAGCGTGACCGTCGGGCCAAACGCGGTGCTGGCTTTCAAACGCGAAGGCTATCGC
AAGCGCGACTTCTCATTTAGCGACACGCTGGAGATTTTGGGCTCGTCGGGGATTCGCCGG
GTGCTGCAAAACCATCTACGCTCAGGACTGGGCGAGATGAAAAACTCGCTGTGCAAAAGC
GGCTATCTGCGGCTGGTGCAAAAGTATTGTCCCCGGCTTTCGTTAAGCGATCTCCAGCCC
TGGCCCGCCGGTGTGCGGGCGCAGGCGGTATCGCCGGACGGCAAGCTGATTGACGATTTT
CTGTTTGTCACCACCCCGCGCACGATCCACACCTGCAATGCGCCCTCCCCGGCAGCGACA
TCAGCAATTCCTATTGGTGCGCATATTGTCAGCAAGGTACAAACGCTGTTGGCAAGCCAG
AGTAACCCCGGACGCACGCTGCGAGCGGCACGTAGTGTGGATGCCTTACACGCCGCATTT
AATCAATAA

Protein Properties

Pfam Domain Function:

Not Available

Protein Residues:

Not Available

Protein Molecular Weight:

Not Available

Protein Theoretical pI:

Not Available

Signaling Regions:

Not Available

Transmembrane Regions:

Not Available

Protein Sequence:

>sp|P37339|LHGO_ECOLI L-2-hydroxyglutarate oxidase LhgO OS=Escherichia coli (strain K12) GN=lhgO PE=1 SV=3
MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESAPACHQTGHNSGVIHAGVYYTPGS
LKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSDLEMERMRALWERTAANGIEREWLN
ADELREREPNITGLGGIFVPSSGIVSYRDVTAAMAKIFQSRGGEIIYNAEVSGLNEHKNG
VVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFIICPFRGEYFRLAPEHNQIVNHL
IYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKREGYRKRDFSFSDTLEILGSSGIRR
VLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLSLSDLQPWPAGVRAQAVSPDGKLIDDF
LFVTTPRTIHTCNAPSPAATSAIPIGAHIVSKVQTLLASQSNPGRTLRAARSVDALHAAF
NQ

References

External Links:

Resource	Link
Uniprot ID:	P37339
ColiBase:	b2660
Kegg Gene:	b2660
BacMap:	90111477

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Borodovsky, M., Rudd, K. E., Koonin, E. V. (1994). "Intrinsic and extrinsic approaches for detecting genes in a bacterial genome." Nucleic Acids Res 22:4756-4767. Pubmed: 7984428
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Kalliri, E., Mulrooney, S. B., Hausinger, R. P. (2008). "Identification of Escherichia coli YgaF as an L-2-hydroxyglutarate oxidase." J Bacteriol 190:3793-3798. Pubmed: 18390652
Metzner, M., Germer, J., Hengge, R. (2004). "Multiple stress signal integration in the regulation of the complex sigma S-dependent csiD-ygaF-gabDTP operon in Escherichia coli." Mol Microbiol 51:799-811. Pubmed: 14731280
Niegemann, E., Schulz, A., Bartsch, K. (1993). "Molecular organization of the Escherichia coli gab cluster: nucleotide sequence of the structural genes gabD and gabP and expression of the GABA permease gene." Arch Microbiol 160:454-460. Pubmed: 8297211
Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837

L-2-hydroxyglutarate oxidase LhgO (P37339)