Coproporphyrinogen-III oxidase, aerobic (P36553)

Identification
Name:Coproporphyrinogen-III oxidase, aerobic
Synonyms:
  • Coprogen oxidase
  • Coproporphyrinogenase
Gene Name:hemF
Enzyme Class:
Biological Properties
General Function:Involved in coproporphyrinogen oxidase activity
Specific Function:Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III
Cellular Location:Cytoplasm
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+2.0Thumb+1.0Thumb ↔ 2.0Thumb+2.0Thumb+1.0Thumb
1.0Coproporphyrin III + 2.0Hydrogen ion + 1.0Oxygen ↔ 2.0Carbon dioxide + 2.0Water + 1.0Protoporphyrinogen IX
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+2.0Thumb+2.0Thumb
1.0Coproporphyrin III + 1.0Oxygen ↔ 1.0Protoporphyrinogen IX + 2.0Carbon dioxide + 2.0Water
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb+2.0Thumb → 1.0Thumb+2.0Thumb+2.0Thumb
1.0Coproporphyrinogen III + 1.0Oxygen + 2.0Hydrogen ion → 1.0Protoporphyrinogen IX + 2.0Carbon dioxide + 2.0Water
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB04030Carbon dioxideMetaboCard
ECMDB00570Coproporphyrin IIIMetaboCard
ECMDB01261Coproporphyrinogen IIIMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB04124OxygenMetaboCard
ECMDB01097Protoporphyrinogen IXMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
catalytic activity
coproporphyrinogen oxidase activity
oxidoreductase activity
oxidoreductase activity, acting on the CH-CH group of donors
oxidoreductase activity, acting on the CH-CH group of donors, oxygen as acceptor
Process
metabolic process
nitrogen compound metabolic process
oxidation reduction
porphyrin biosynthetic process
porphyrin metabolic process
tetrapyrrole metabolic process
Gene Properties
Blattner:b2436
Gene OrientationClockwise
Centisome Percentage:54.99
Left Sequence End2551247
Right Sequence End2552146
Gene Sequence:
>900 bp
ATGAAGCGTGAAGAAATCGCTGATCTGATGGCGTTTGTCGTCGTTGCAGAGGAGCGTAGC
TTCACTCGTGCAGCAGCCCGCCTGAGCATGGCGCAGTCAGCTTTAAGCCAGATAGTGCGT
CGTATAGAAGAACGATTGGGATTGCGGCTTCTGACGCGAACCACGCGCAGCGTTGTTCCA
ACTGAAGCGGGCGAGCATCTTTTGTCTGTTCTTGGCCCTATGTTGCATGACATAGATTCA
GCCATGGCATCCCTGAGCGATCTGCAGAACCGCCCATCCGGGACAATACGTATTACTACT
GTAGAACATGCAGCAAAAACGATATTGTTACCAGCAATGCGCACATTCCTGAAATCGCAT
CCTGAAATTGATATTCAGCTCACCATTGATTATGGTTTGACCGATGTCGTTTCTGAACGT
TTTGATGCAGGCGTCCGTCTGGGTGGGGAGATGGATAAAGATATGATCGCCATTCGAATC
GGGCCAGATATACCAATGGCTATTGTTGGCTCACCGGATTATTTTTCTCGCCGAAGTGTT
CCAACGTCAGTGTCACAATTAATAGATCATCAGGCAATTAATTTGTATCTTCCCACATCG
GGTACAGCAAATCGCTGGAGATTAATACGCGGTGGACGTGAAGTTCGTGTTCGCATGGAA
GGTCAGCTTTTACTGAATACGATAGACCTGATCATTGATGCTGCAATTGATGGGCATGGA
TTGGCGTATCTACCTTATGATCAGGTTGAGCGGGCTATTAAAGAAAAAAAACTGATACGT
GTTTTGGATAAATTCACACCAGATTTACCCGGTTATCACCTGTACTATCCACACCGTCGA
CATGCTGGCTCGGCATTCTCATTATTTATAGATAGGCTGAAGTATAAAGGTGCTGTTTAG
Protein Properties
Pfam Domain Function:
Protein Residues:299
Protein Molecular Weight:34322
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Coproporphyrinogen-III oxidase, aerobic
MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGGRSRVLRNGGVFEQAGV
NFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYVPTSHANVRFFIAEKPGAD
PVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVYPRYKKWCDEYFYLKHRNEQR
GIGGLFFDDLNTPDFDRCFAFMQAVGKGYTDAYLPIVERRKAMAYGERERNFQLYRRGRY
VEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEYDYQPKDGSPEAALSEFIKVRDWV
References
External Links:
ResourceLink
Uniprot ID:P36553
Uniprot Name:HEM6_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1742185
Ecogene ID:EG12189
Ecocyc:EG12189
ColiBase:b2436
Kegg Gene:b2436
EchoBASE ID:EB2106
CCDB:HEM6_ECOLI
BacMap:16130361
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Breckau, D., Mahlitz, E., Sauerwald, A., Layer, G., Jahn, D. (2003). "Oxygen-dependent coproporphyrinogen III oxidase (HemF) from Escherichia coli is stimulated by manganese." J Biol Chem 278:46625-46631. Pubmed: 12975365
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Macieira, S., Martins, B. M., Huber, R. (2003). "Oxygen-dependent coproporphyrinogen-III oxidase from Escherichia coli: one-step purification and biochemical characterisation." FEMS Microbiol Lett 226:31-37. Pubmed: 13129604
  • Troup, B., Jahn, M., Hungerer, C., Jahn, D. (1994). "Isolation of the hemF operon containing the gene for the Escherichia coli aerobic coproporphyrinogen III oxidase by in vivo complementation of a yeast HEM13 mutant." J Bacteriol 176:673-680. Pubmed: 8300522
  • Yamamoto, Y., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Itoh, T., Kimura, S., Kitagawa, M., Makino, K., Miki, T., Mitsuhashi, N., Mizobuchi, K., Mori, H., Nakade, S., Nakamura, Y., Nashimoto, H., Oshima, T., Oyama, S., Saito, N., Sampei, G., Satoh, Y., Sivasundaram, S., Tagami, H., Horiuchi, T., et, a. l. .. (1997). "Construction of a contiguous 874-kb sequence of the Escherichia coli -K12 genome corresponding to 50.0-68.8 min on the linkage map and analysis of its sequence features." DNA Res 4:91-113. Pubmed: 9205837