Identification
Name:tRNA 2-selenouridine synthase
Synonyms:
  • Selenophosphate-dependent tRNA 2-selenouridine synthase
Gene Name:selU
Enzyme Class:Not Available
Biological Properties
General Function:Involved in transferase activity, transferring selenium-containing groups
Specific Function:Catalyzes the transfer of selenium from selenophosphate for conversion of 2-thiouridine to 2-selenouridine at the wobble position in tRNA
Cellular Location:Not Available
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
EcoCyc Reactions:
1.0tRNA containing 5-methylaminomethyl-2-thiouridylate+1.0Thumb1.0unknown+1.0tRNA containing 5-methylaminomethyl-2-selenouridine
1.0tRNA containing 5-methylaminomethyl-2-thiouridylate + 1.0Selenophosphate → 1.0unknown + 1.0tRNA containing 5-methylaminomethyl-2-selenouridine
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb
Metabolites:
ECMDB IDNameView
ECMDB202375-Methylaminomethyl-2-selenouridineMetaboCard
ECMDB202385-Methylaminomethyl-2-thiouridineMetaboCard
ECMDB21380Inorganic phosphateMetaboCard
ECMDB03840Phosphoroselenoic acidMetaboCard
ECMDB21449SelenophosphateMetaboCard
GO Classification:
Function
binding
catalytic activity
selenium binding
selenotransferase activity
transferase activity
transferase activity, transferring selenium-containing groups
Process
cellular metabolic process
metabolic process
selenium metabolic process
sulfur metabolic process
Gene Properties
Blattner:b0503
Gene OrientationCounterclockwise
Centisome Percentage:11.41
Left Sequence End529356
Right Sequence End530450
Gene Sequence:
>1095 bp
ATGTCGCGCGTCAATCCTCTATCTTCGCTGTCATTGTTGGCGGTTCTGGTTTTAGCGGGG
TGTAGTAGTCAGGCTCCGCAACCGTTGAAAAAGGGCGAAAAAGCGATAGATGTAGCGAGC
GTCGTGCGGCAGAAGATGCCTGCGAGCGTGAAAGACCGCGATGCCTGGGCGAAAGATCTC
GCCACCACCTTTGAAAGTCAGGGGCTGGCACCGACGCTGGAAAACGTCTGCTCGGTGCTG
GCGGTGGCGCAGCAGGAGTCAAATTATCAGGCAGATCCGGCCGTTCCGGGCTTAAGCAAG
ATCGCCTGGCAAGAGATTGACCGTCGTGCTGAACGGATGCACATTCCTGCTTTTCTGGTT
CATACCGCGCTAAAAATCAAATCGCCAAACGGCAAAAGTTATAGCGAACGGTTAGATTCG
GTGCGTACAGAAAAGCAATTGAGCGCGATTTTTGACGATCTGATCAACATGGTGCCAATG
GGGCAGACGCTGTTTGGTTCGCTCAATCCGGTGCGCACCGGTGGGCCGATGCAGGTCAGC
ATTGCTTTTGCCGAACAGCATACCAAAGGGTATCCGTGGAAAATGGACGGTACAGTCCGT
CAGGAAGTCTTCAGTCGCCGCGGCGGGTTGTGGTTTGGTACTTACCATTTACTGAATTAT
CCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCC
AGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTG
GACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGC
AAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAGAGAAAGGT
GACAGCTTCTCTTTTGAGGAGACGGCGCTGTACAAGAAAGTTTATCAACTTGCCGAAACG
AAAACGGGTAAATCACTCCCCCGCGAAATGTTGCCTGGCATTCAACTGGAAAGCCCGAAA
ATCACCCGCAACCTGACTACGGCCTGGTTTGCGAAGCGCGTAGACGAACGGCGGGCGCGT
TGTATGAAACAGTGA
Protein Properties
Pfam Domain Function:
Protein Residues:364
Protein Molecular Weight:41110
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>tRNA 2-selenouridine synthase
MQERHTEQDYRALLIADTPIIDVRAPIEFEHGAMPAAINLPLMNNDERAAVGTCYKQQGS
DAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQRSHIVQSWLHAAGIDYPL
VEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQQQPNGVDLEGLARHRGSAFG
RTLQPQLSQASFENLLAAEMLKTDARQNLRLWVLEDESRMIGSNHLPECLRERMTQAAIA
VVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQEYCEYLHHGLSAIKRRLGLQRYNE
LAARLDAALTTQLTTGSTDGHLAWLVPLLEEYYDPMYRYQLEKKAEKVVFRGEWAEVAEW
VKAR
References
External Links:
ResourceLink
Uniprot ID:P33667
Uniprot Name:SELU_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674519
Ecogene ID:EG11768
Ecocyc:EG11768
ColiBase:b0503
Kegg Gene:b0503
EchoBASE ID:EB1718
CCDB:SELU_ECOLI
BacMap:16128487
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Sadosky, A. B., Gray, J. A., Hill, C. W. (1991). "The RhsD-E subfamily of Escherichia coli K-12." Nucleic Acids Res 19:7177-7183. Pubmed: 1766878
  • Wolfe, M. D., Ahmed, F., Lacourciere, G. M., Lauhon, C. T., Stadtman, T. C., Larson, T. J. (2004). "Functional diversity of the rhodanese homology domain: the Escherichia coli ybbB gene encodes a selenophosphate-dependent tRNA 2-selenouridine synthase." J Biol Chem 279:1801-1809. Pubmed: 14594807