Periplasmic beta-glucosidase (P33363)

Identification
Name:Periplasmic beta-glucosidase
Synonyms:
  • Beta-D-glucoside glucohydrolase
  • Cellobiase
  • Gentiobiase
Gene Name:bglX
Enzyme Class:
Biological Properties
General Function:Involved in hydrolase activity, hydrolyzing O-glycosyl compounds
Specific Function:Hydrolysis of terminal, non-reducing beta-D- glucosyl residues with release of beta-D-glucose
Cellular Location:Periplasm
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 2.0Thumb
1.0Cellobiose + 1.0Water ↔ 2.0b-D-Glucose
ReactionCard
1.0Cellulose+1.0Thumb ↔ 1.0Cellulose+1.0Thumb
1.0Cellulose + 1.0Water ↔ 1.0Cellulose + 1.0b-D-Glucose
ReactionCard
1.0D-Glucoside+1.0Thumb ↔ 1.0ROH+1.0Thumb
1.0D-Glucoside + 1.0Water ↔ 1.0ROH + 1.0alpha-D-Glucose
ReactionCard
1.0Cyanoglycoside+1.0Thumb ↔ 1.0Cyanohydrin+1.0Thumb
1.0Cyanoglycoside + 1.0Water ↔ 1.0Cyanohydrin + 1.0D-Glucose
ReactionCard
1.0cis-beta-D-Glucosyl-2-hydroxycinnamate+1.0Thumb ↔ 1.0cis-2-Hydroxycinnamate+1.0Thumb
1.0cis-beta-D-Glucosyl-2-hydroxycinnamate + 1.0Water ↔ 1.0cis-2-Hydroxycinnamate + 1.0D-Glucose
ReactionCard
EcoCyc Reactions:
1.0a β-D glucoside+1.0Thumb ? 1.0a non glucosylated glucose acceptor+1.0Thumb
1.0a β-D glucoside + 1.0Water ? 1.0a non glucosylated glucose acceptor + 1.0b-D-Glucose
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB03345alpha-D-GlucoseMetaboCard
ECMDB00186alpha-LactoseMetaboCard
ECMDB00516b-D-GlucoseMetaboCard
ECMDB00055CellobioseMetaboCard
ECMDB00143D-GalactoseMetaboCard
ECMDB00122D-GlucoseMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
catalytic activity
hydrolase activity
hydrolase activity, acting on glycosyl bonds
hydrolase activity, hydrolyzing O-glycosyl compounds
Process
carbohydrate metabolic process
metabolic process
primary metabolic process
Gene Properties
Blattner:b2132
Gene OrientationCounterclockwise
Centisome Percentage:47.80
Left Sequence End2217714
Right Sequence End2220011
Gene Sequence:
>2298 bp
ATGAAATGGCTATGTTCAGTAGGAATCGCGGTGAGTCTGGCCCTGCAGCCAGCACTGGCG
GATGATTTATTCGGCAACCATCCATTAACGCCCGAAGCGCGGGATGCGTTCGTCACCGAA
CTGCTTAAGAAAATGACAGTTGATGAGAAAATTGGTCAGCTGCGCTTAATCAGCGTCGGC
CCGGATAACCCGAAAGAGGCGATCCGCGAGATGATCAAAGACGGTCAGGTTGGGGCGATT
TTCAACACCGTAACCCGTCAGGATATCCGCGCCATGCAGGATCAGGTGATGGAATTAAGC
CGCCTGAAAATTCCTCTTTTCTTTGCTTACGACGTGCTGCACGGTCAGCGCACGGTGTTC
CCGATTAGCCTCGGTCTGGCCTCGTCTTTTAACCTCGATGCAGTGAAAACGGTCGGACGT
GTCTCTGCTTATGAAGCGGCAGATGATGGCCTGAATATGACCTGGGCACCGATGGTCGAT
GTCTCGCGCGATCCGCGCTGGGGACGTGCTTCCGAAGGTTTTGGCGAAGATACGTATCTC
ACCTCAACAATGGGTAAAACCATGGTGGAAGCGATGCAGGGTAAAAGCCCGGCAGATCGC
TACTCGGTGATGACCAGCGTCAAACACTTTGCCGCATACGGCGCGGTAGAAGGCGGTAAA
GAGTACAACACCGTCGATATGAGTCCGCAGCGCCTGTTTAATGATTATATGCCGCCGTAC
AAAGCGGGGCTGGACGCAGGCAGCGGCGCGGTGATGGTGGCGCTGAACTCGCTGAACGGC
ACGCCAGCCACCTCCGATTCCTGGCTGCTGAAAGATGTTCTGCGCGACCAGTGGGGCTTT
AAAGGCATCACCGTTTCCGATCACGGTGCAATCAAAGAGCTGATTAAACATGGCACGGCG
GCAGACCCGGAAGATGCGGTGCGCGTGGCGCTGAAATCCGGAATCAACATGAGCATGAGC
GACGAGTACTACTCGAAGTATCTGCCTGGGTTGATTAAATCCGGCAAAGTGACGATGGCA
GAGCTGGACGATGCTGCCCGCCATGTACTGAACGTTAAATATGATATGGGGTTGTTTAAC
GACCCATACAGCCATTTGGGGCCGAAAGAGTCTGACCCGGTGGATACCAATGCCGAAAGC
CGCCTGCACCGTAAAGAAGCGCGTGAAGTGGCGCGCGAAAGCTTGGTGTTGCTGAAAAAC
CGTCTCGAAACGTTACCGCTGAAAAAATCGGCCACCATTGCGGTGGTTGGGCCACTGGCG
GACAGTAAACGTGACGTGATGGGCAGCTGGTCCGCAGCCGGTGTTGCCGATCAATCCGTG
ACCGTACTGACCGGGATTAAAAATGCCGTCGGTGAAAACGGTAAAGTGCTGTATGCCAAA
GGGGCGAACGTTACCAGTGACAAAGGCATTATCGATTTCCTGAATCAGTATGAAGAAGCG
GTCAAAGTCGATCCGCGTTCGCCGCAAGAGATGATTGATGAAGCGGTGCAGACGGCGAAA
CAATCTGATGTGGTGGTGGCTGTAGTCGGTGAAGCACAGGGGATGGCGCACGAAGCCTCC
AGCCGGACCGATATCACTATTCCGCAAAGCCAACGTGACTTGATTGCGGCGCTGAAAGCC
ACCGGTAAACCGCTGGTGCTGGTGCTGATGAACGGGCGTCCGCTGGCGCTGGTGAAAGAA
GATCAGCAGGCTGATGCGATTCTGGAAACCTGGTTTGCGGGGACTGAAGGCGGTAATGCA
ATTGCCGATGTATTGTTTGGCGATTACAACCCGTCCGGCAAGCTGCCAATGTCCTTCCCG
CGTTCTGTCGGGCAGATCCCGGTGTACTACAGCCATCTGAATACCGGTCGCCCGTATAAT
GCCGACAAGCCGAACAAATACACTTCGCGTTATTTTGATGAAGCTAACGGGGCGTTGTAT
CCGTTCGGCTATGGGCTGAGCTACACCACTTTCACCGTCTCTGATGTGAAACTTTCTGCG
CCGACCATGAAGCGTGACGGCAAAGTGACTGCCAGCGTGCAGGTGACGAACACCGGTAAG
CGCGAGGGTGCCACGGTAGTGCAGATGTACTTGCAGGATGTGACGGCTTCCATGAGTCGC
CCTGTGAAACAGCTGAAAGGCTTTGAGAAAATCACCCTGAAACCGGGCGAAACTCAGACT
GTCAGCTTCCCGATCGATATTGAGGCGCTGAAGTTCTGGAATCAACAGATGAAATATGAC
GCCGAGCCTGGCAAGTTCAATGTCTTTATCGGCACTGATTCCGCACGCGTTAAGAAAGGC
GAGTTTGAGTTGCTGTAA
Protein Properties
Pfam Domain Function:
Protein Residues:765
Protein Molecular Weight:83459
Protein Theoretical pI:6
Signaling Regions:
  • 1-20
Transmembrane Regions:
  • None
Protein Sequence:
>Periplasmic beta-glucosidase
MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKKMTVDEKIGQLRLISVG
PDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRLKIPLFFAYDVLHGQRTVF
PISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVDVSRDPRWGRASEGFGEDTYL
TSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEGGKEYNTVDMSPQRLFNDYMPPY
KAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRDQWGFKGITVSDHGAIKELIKHGTA
ADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKSGKVTMAELDDAARHVLNVKYDMGLFN
DPYSHLGPKESDPVDTNAESRLHRKEAREVARESLVLLKNRLETLPLKKSATIAVVGPLA
DSKRDVMGSWSAAGVADQSVTVLTGIKNAVGENGKVLYAKGANVTSDKGIIDFLNQYEEA
VKVDPRSPQEMIDEAVQTAKQSDVVVAVVGEAQGMAHEASSRTDITIPQSQRDLIAALKA
TGKPLVLVLMNGRPLALVKEDQQADAILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFP
RSVGQIPVYYSHLNTGRPYNADKPNKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSA
PTMKRDGKVTASVQVTNTGKREGATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQT
VSFPIDIEALKFWNQQMKYDAEPGKFNVFIGTDSARVKKGEFELL
References
External Links:
ResourceLink
Uniprot ID:P33363
Uniprot Name:BGLX_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85675246
Ecogene ID:EG12013
Ecocyc:EG12013
ColiBase:b2132
Kegg Gene:b2132
EchoBASE ID:EB1951
CCDB:BGLX_ECOLI
BacMap:16130070
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553