Trehalose-phosphate phosphatase (P31678)

Identification
Name:Trehalose-phosphate phosphatase
Synonyms:
  • TPP
  • Osmoregulatory trehalose synthesis protein B
  • Trehalose 6-phosphate phosphatase
  • Trehalose-phosphatase
Gene Name:otsB
Enzyme Class:
Biological Properties
General Function:Involved in catalytic activity
Specific Function:Removes the phosphate from trehalose 6-phosphate to produce free trehalose
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Water + 1.0Trehalose 6-phosphate → 1.0Phosphate + 1.0Trehalose
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Trehalose 6-phosphate + 1.0Water ↔ 1.0Trehalose + 1.0Phosphate
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Trehalose 6-phosphate + 1.0Water → 1.0Phosphate + 1.0α,α-trehalose
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0alpha,alpha-Trehalose 6-phosphate + 1.0Water → 1.0α,α-trehalose + 1.0Phosphate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Water + 1.0Trehalose 6-phosphate → 1.0Phosphate + 1.0Trehalose
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Trehalose 6-phosphate + 1.0Water → 1.0Trehalose + 1.0Inorganic phosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB04010alpha,alpha-Trehalose 6-phosphateMetaboCard
ECMDB21380Inorganic phosphateMetaboCard
ECMDB01429PhosphateMetaboCard
ECMDB00975TrehaloseMetaboCard
ECMDB01124Trehalose 6-phosphateMetaboCard
ECMDB00494WaterMetaboCard
ECMDB24256α,α-trehaloseMetaboCard
GO Classification:
Function
catalytic activity
Process
carbohydrate metabolic process
disaccharide metabolic process
metabolic process
oligosaccharide metabolic process
primary metabolic process
trehalose biosynthetic process
trehalose metabolic process
Gene Properties
Blattner:b1897
Gene OrientationCounterclockwise
Centisome Percentage:42.67
Left Sequence End1979611
Right Sequence End1980411
Gene Sequence:
>801 bp
GTGCGAAAAAACACCTATGCCATGCGCTATGTTGCCGGACAACCTGCGGAAAGGATCTTA
CCGCCGGGGTCTTTTGCGAGCATCGGCCAGGCATTACCACCTGGGGAACCGTTAAGTACC
GAAGAGCGTATTCGGATCCTGGTGTGGAACATATACAAACAGCAACGCGCTGAATGGTTG
TCGGTATTAAAGAACTACGGCAAAGATGCACATCTGGTGTTATTGCAGGAAGCGCAGACA
ACGCCAGAGTTAGTACAGTTTGCGACCGCTAACTATCTTGCCGCCGATCAGGTACCCGCT
TTCGTGCTGCCACAACATCCTTCTGGCGTAATGACCCTTTCGGCGGCACATCCAGTGTAT
TGCTGCCCGTTACGTGAACGAGAACCCATTTTGCGTCTGGCGAAGTCGGCACTGGTGACG
GTCTATCCATTACCTGACACCCGCCTGTTGATGGTGGTTAATATACACGCCGTCAACTTC
AGTCTGGGCGTGGATGTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCAC
CACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAAC
GCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGATGATCAG
CGCCGTCGGGCGTTTGGTCGCCCGCTCGATTTTGTTTTCTACCGTGGTCTGAACGTCAGT
GAAGCTTCTGTACTGGTTACGCGCGCTTCCGATCACAATCCGCTACTCGTTGAATTCAGT
CCCGGCAAGCCTGATAAATAA
Protein Properties
Pfam Domain Function:
Protein Residues:266
Protein Molecular Weight:29175
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Trehalose-phosphate phosphatase
MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQGLQLLATASDGALALIS
GRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAIARDISVQLHTVIAQYPGA
ELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQQGKCVVEIKPRGTSKGEAIA
AFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVKIGTGATQASWRLAGVPDVWSWL
EMITTALQQKRENNRSDDYESFSRSI
References
External Links:
ResourceLink
Uniprot ID:P31678
Uniprot Name:OTSB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674383
Ecogene ID:EG11752
Ecocyc:EG11752
ColiBase:b1897
Kegg Gene:b1897
EchoBASE ID:EB1702
CCDB:OTSB_ECOLI
BacMap:16129849
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Itoh, T., Aiba, H., Baba, T., Hayashi, K., Inada, T., Isono, K., Kasai, H., Kimura, S., Kitakawa, M., Kitagawa, M., Makino, K., Miki, T., Mizobuchi, K., Mori, H., Mori, T., Motomura, K., Nakade, S., Nakamura, Y., Nashimoto, H., Nishio, Y., Oshima, T., Saito, N., Sampei, G., Seki, Y., Horiuchi, T., et, a. l. .. (1996). "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 40.1-50.0 min region on the linkage map." DNA Res 3:379-392. Pubmed: 9097040
  • Kaasen, I., McDougall, J., Strom, A. R. (1994). "Analysis of the otsBA operon for osmoregulatory trehalose synthesis in Escherichia coli and homology of the OtsA and OtsB proteins to the yeast trehalose-6-phosphate synthase/phosphatase complex." Gene 145:9-15. Pubmed: 8045430