Probable adenine permease PurP (P31466)

Identification
Name:Probable adenine permease PurP
Synonyms:Not Available
Gene Name:purP
Enzyme Class:Not Available
Biological Properties
General Function:Involved in transporter activity
Specific Function:High-affinity uptake of adenine
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:
KEGG Pathways:Not Available
Transports:
Transport References:
  • Orth, J. D., Conrad, T. M., Na, J., Lerman, J. A., Nam, H., Feist, A. M., Palsson, B. O. (2011). "A comprehensive genome-scale reconstruction of Escherichia coli metabolism--2011." Mol Syst Biol 7:535. Pubmed: 21988831
  • Uniprot Consortium (2012). "Reorganizing the protein space at the Universal Protein Resource (UniProt)." Nucleic Acids Res 40:D71-D75. Pubmed: 22102590
Metabolites:
ECMDB IDNameView
ECMDB00034AdenineMetaboCard
ECMDB21225Hydrogen ionMetaboCard
GO Classification:
Component
cell part
membrane
Function
transporter activity
Process
establishment of localization
transmembrane transport
transport
Gene Properties
Blattner:b3714
Gene OrientationCounterclockwise
Centisome Percentage:83.91
Left Sequence End3893295
Right Sequence End3894632
Gene Sequence:
>1338 bp
ATGCACTCTCAAATCTGGGTTGTGAGCACGCTGCTTATCAGCATCGTGTTAATTGTACTG
ACCATCGTGAAGTTCAAATTCCACCCGTTTCTGGCGCTGTTGCTGGCCAGCTTCTTCGTG
GGAACGATGATGGGCATGGGGCCACTGGATATGGTAAATGCTATTGAAAGTGGAATTGGC
GGAACGCTGGGGTTCCTCGCAGCGGTTATCGGCCTTGGCACGATACTGGGAAAAATGATG
GAAGTATCCGGGGCCGCAGAAAGAATTGGTCTGACACTTCAACGCTGCCGCTGGCTTTCA
GTTGATGTCATTATGGTGCTGGTTGGCCTGATTTGTGGCATCACGCTGTTTGTTGAAGTG
GGCGTCGTGCTATTGATTCCTCTGGCTTTTTCAATTGCCAAAAAAACCAATACCTCATTA
TTAAAGCTTGCCATTCCGCTATGTACCGCATTGATGGCAGTGCACTGCGTGGTTCCTCCA
CATCCGGCTGCTTTATATGTTGCCAATAAGCTGGGCGCAGATATCGGTTCGGTGATCGTC
TACGGTTTGCTGGTTGGGCTGATGGCATCACTGATCGGTGGCCCACTTTTCCTTAAATTT
CTGGGTCAACGACTGCCCTTTAAACCTGTACCCACAGAGTTTGCAGATCTCAAAGTTCGC
GATGAAAAAACACTACCGTCATTAGGCGCAACGTTATTCACCATACTGCTACCCATTGCG
CTGATGTTGGTTAAAACGATTGCCGAATTGAATATGGCGCGTGAGAGTGGTTTGTATATC
TTGGTTGAGTTTATTGGCAACCCTATCACTGCCATGTTTATCGCCGTGTTTGTCGCCTAT
TATGTGTTGGGTATACGCCAGCATATGAGCATGGGGACGATGCTCACACATACGGAAAAT
GGCTTCGGTTCTATTGCTAATATTTTGCTGATTATCGGGGCCGGAGGCGCATTCAACGCC
ATTTTAAAAAGCAGCAGTCTCGCTGATACGCTGGCAGTTATTCTCTCCAATATGCATATG
CACCCGATTCTTCTGGCCTGGTTAGTGGCTCTTATTCTGCATGCGGCAGTGGGCTCCGCT
ACCGTGGCAATGATGGGGGCAACGGCAATTGTTGCACCCATGCTGCCGCTGTATCCCGAC
ATCAGCCCGGAAATTATTGCGATTGCTATCGGTTCAGGTGCAATTGGCTGCACTATCGTT
ACGGACTCGCTTTTCTGGCTAGTGAAGCAATATTGCGGCGCTACGCTCAATGAAACATTT
AAATACTATACGACAGCGACATTTATCGCTTCAGTCGTCGCTCTGGCGGGCACATTCCTG
CTGTCATTTATCATCTAA
Protein Properties
Pfam Domain Function:
Protein Residues:445
Protein Molecular Weight:46865
Protein Theoretical pI:8
Signaling Regions:
  • None
Transmembrane Regions:
  • 32-52
  • 62-82
  • 86-106
  • 110-130
  • 148-168
  • 180-200
  • 202-222
  • 246-266
  • 295-317
  • 327-349
  • 356-374
  • 389-409
  • 424-444
Protein Sequence:
>Probable adenine permease PurP
MSHQHTTQTSGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVYIVFVNPQILGVAGMDT
SAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVVVQAMGLPWQVGMGAIFWG
AIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMGLKNAGVIVANPETLVSIGNL
TSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLGWMLGDVHYNGIVSAPPSVMTVV
GHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVTDKAGLADEKGKFPRMKQALYVDSI
SSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAVVVGLLFLLVIFLSPLAGMVPGYAAAG
ALIYVGVLMTSSLARVNWQDLTESVPAFITAVMMPFSFSITEGIALGFISYCVMKIGTGR
LRDLSPCVIIVALLFILKIVFIDAH
References
External Links:
ResourceLink
Uniprot ID:P31466
Uniprot Name:PURP_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1799764
Ecogene ID:EG11724
Ecocyc:EG11724
ColiBase:b3714
Kegg Gene:b3714
EchoBASE ID:EB1675
CCDB:PURP_ECOLI
BacMap:16131582
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Burland, V., Plunkett, G. 3rd, Daniels, D. L., Blattner, F. R. (1993). "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication." Genomics 16:551-561. Pubmed: 7686882
  • Burton, K. (1983). "Transport of nucleic acid bases into Escherichia coli." J Gen Microbiol 129:3505-3513. Pubmed: 6198438
  • Burton, K. (1994). "Adenine transport in Escherichia coli." Proc Biol Sci 255:153-157. Pubmed: 8165228
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553