3-isopropylmalate dehydratase small subunit (P30126)

Identification
Name:3-isopropylmalate dehydratase small subunit
Synonyms:
  • Alpha-IPM isomerase
  • IPMI
  • Isopropylmalate isomerase
Gene Name:leuD
Enzyme Class:
Biological Properties
General Function:Involved in 3-isopropylmalate dehydratase activity
Specific Function:Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate
Cellular Location:Not Available
SMPDB Pathways:
  • Leucine Biosynthesis PW000811
  • Operon: leucine biosynthesis PW001880
  • Secondary Metabolite: Leucine biosynthesis PW000980
  • Secondary Metabolites: Valine and I-leucine biosynthesis from pyruvate PW000978
KEGG Pathways:
KEGG Reactions:
1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.03-Isopropylmalate ↔ 1.0Isopropylmaleate + 1.0Water
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb
1.0Isopropylmaleate + 1.0Water ↔ 1.02-Isopropylmalic acid
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1.0(R)-2-Methylmalate ↔ 1.0Thumb+1.0Thumb
1.0(R)-2-Methylmalate ↔ 1.0Citraconic acid + 1.0Water
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1.0Thumb+1.0Thumb ↔ 1.0Thumb
1.0Citraconic acid + 1.0Water ↔ 1.0D-Erythro-3-Methylmalate
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1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.02-Isopropylmalic acid ↔ 1.0Isopropylmaleate + 1.0Water
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1.0Thumb+1.0Thumb+1.0Thumb ↔ 1.0Thumb
1.03-Isopropylmalate + 1.0Isopropylmaleate + 1.0Water ↔ 1.02-Isopropylmalic acid
ReactionCard
SMPDB Reactions:
1.0Thumb → 1.0Thumb+1.0Thumb
1.02-Isopropylmalic acid → 1.0Water + 1.0Isopropylmaleate
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1.0Thumb+1.0Thumb → 1.0Thumb
1.0Isopropylmaleate + 1.0Water → 1.03-Isopropylmalate
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1.0Thumb+1.0Thumb → 1.0Citramalic acid
1.0Citraconic acid + 1.0Water → 1.0Citramalic acid
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EcoCyc Reactions:
1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.03-Isopropylmalate ↔ 1.0Isopropylmaleate + 1.0Water
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB004022-Isopropylmalic acidMetaboCard
ECMDB041583-IsopropylmalateMetaboCard
ECMDB21397Citraconic acidMetaboCard
ECMDB20137D-Erythro-3-MethylmalateMetaboCard
ECMDB12241IsopropylmaleateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
3-isopropylmalate dehydratase complex
macromolecular complex
protein complex
Function
3-isopropylmalate dehydratase activity
carbon-oxygen lyase activity
catalytic activity
hydro-lyase activity
lyase activity
Process
branched chain family amino acid metabolic process
cellular amino acid and derivative metabolic process
cellular amino acid metabolic process
cellular metabolic process
leucine biosynthetic process
leucine metabolic process
metabolic process
Gene Properties
Blattner:b0071
Gene OrientationCounterclockwise
Centisome Percentage:1.70
Left Sequence End78848
Right Sequence End79453
Gene Sequence:
>606 bp
ATGGCAGAGAAATTTATCAAACACACAGGCCTGGTGGTTCCGCTGGATGCCGCCAATGTC
GATACCGATGCAATCATCCCGAAACAGTTTTTGCAGAAAGTGACCCGTACGGGTTTTGGC
GCGCATCTGTTTAACGACTGGCGTTTTCTGGATGAAAAAGGCCAACAGCCAAACCCGGAC
TTCGTGCTGAACTTCCCGCAGTATCAGGGCGCTTCCATTTTGCTGGCACGAGAAAACTTC
GGCTGTGGCTCTTCGCGTGAGCACGCGCCCTGGGCATTGACCGACTACGGTTTTAAAGTG
GTGATTGCGCCGAGTTTTGCTGACATCTTCTACGGCAATAGCTTTAACAACCAGCTGCTG
CCGGTGAAATTAAGCGATGCAGAAGTGGACGAACTGTTTGCGCTGGTGAAAGCTAATCCG
GGGATCCATTTCGACGTGGATCTGGAAGCGCAAGAGGTGAAAGCGGGAGAGAAAACCTAT
CGCTTTACCATCGATGCCTTCCGCCGCCACTGCATGATGAACGGTCTGGACAGTATTGGG
CTTACCTTGCAGCACGACGACGCCATTGCCGCTTATGAAGCAAAACAACCTGCGTTTATG
AATTAA
Protein Properties
Pfam Domain Function:
Protein Residues:201
Protein Molecular Weight:22487
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>3-isopropylmalate dehydratase small subunit
MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLFNDWRFLDEKGQQPNPD
FVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVIAPSFADIFYGNSFNNQLL
PVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTYRFTIDAFRRHCMMNGLDSIG
LTLQHDDAIAAYEAKQPAFMN
References
External Links:
ResourceLink
Uniprot ID:P30126
Uniprot Name:LEUD_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:21321953
Ecogene ID:EG11575
Ecocyc:EG11575
ColiBase:b0071
Kegg Gene:b0071
EchoBASE ID:EB1535
CCDB:LEUD_ECOLI
BacMap:16128065
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Wilkins, M. R., Gasteiger, E., Tonella, L., Ou, K., Tyler, M., Sanchez, J. C., Gooley, A. A., Walsh, B. J., Bairoch, A., Appel, R. D., Williams, K. L., Hochstrasser, D. F. (1998). "Protein identification with N and C-terminal sequence tags in proteome projects." J Mol Biol 278:599-608. Pubmed: 9600841
  • Yura, T., Mori, H., Nagai, H., Nagata, T., Ishihama, A., Fujita, N., Isono, K., Mizobuchi, K., Nakata, A. (1992). "Systematic sequencing of the Escherichia coli genome: analysis of the 0-2.4 min region." Nucleic Acids Res 20:3305-3308. Pubmed: 1630901