Uroporphyrinogen decarboxylase (P29680)

Identification
Name:Uroporphyrinogen decarboxylase
Synonyms:
  • UPD
  • URO-D
Gene Name:hemE
Enzyme Class:
Biological Properties
General Function:Involved in uroporphyrinogen decarboxylase activity
Specific Function:Catalyzes the decarboxylation of four acetate groups of uroporphyrinogen-III to yield coproporphyrinogen-III
Cellular Location:Cytoplasm (Probable)
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
4.0Thumb+1.0Thumb ↔ 4.0Thumb+1.0Thumb
4.0Hydrogen ion + 1.0Uroporphyrinogen III ↔ 4.0Carbon dioxide + 1.0Coproporphyrin III
ReactionCard
1.0Thumb ↔ 1.0Thumb+4.0Thumb
1.0Uroporphyrinogen III ↔ 1.0Coproporphyrin III + 4.0Carbon dioxide
ReactionCard
1.0Thumb ↔ 1.0Thumb+4.0Thumb
1.0Uroporphyrinogen I ↔ 1.0Coproporphyrinogen I + 4.0Carbon dioxide
ReactionCard
SMPDB Reactions:
1.0Thumb+4.0Thumb → 4.0Thumb+1.0Thumb
1.0Uroporphyrinogen III + 4.0Hydrogen ion → 4.0Carbon dioxide + 1.0Coproporphyrinogen III
ReactionCard
1.0Thumb+4.0Thumb → 4.0Thumb+1.0Thumb
1.0Uroporphyrinogen I + 4.0Hydrogen ion → 4.0Carbon dioxide + 1.0Coproporphyrinogen I
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Hydrogen ion + 1.0Uroporphyrinogen III → 1.0Carbon dioxide + 1.0Coproporphyrinogen III
ReactionCard
Complex Reactions:
1.0Thumb → 1.0Thumb+4.0Thumb
1.0Uroporphyrinogen III → 1.0Coproporphyrinogen III + 4.0Carbon dioxide
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB04030Carbon dioxideMetaboCard
ECMDB00570Coproporphyrin IIIMetaboCard
ECMDB02158Coproporphyrinogen IMetaboCard
ECMDB01261Coproporphyrinogen IIIMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB02211Uroporphyrinogen IMetaboCard
ECMDB01086Uroporphyrinogen IIIMetaboCard
GO Classification:
Function
carbon-carbon lyase activity
carboxy-lyase activity
catalytic activity
lyase activity
uroporphyrinogen decarboxylase activity
Process
metabolic process
nitrogen compound metabolic process
porphyrin biosynthetic process
porphyrin metabolic process
tetrapyrrole metabolic process
Gene Properties
Blattner:b3997
Gene OrientationClockwise
Centisome Percentage:90.43
Left Sequence End4195739
Right Sequence End4196803
Gene Sequence:
>1065 bp
ATGGTAACGTCCTGTACTGGACATGTTTTAGATAATCAACGCGCCACCACTCGCGGAGTT
TTCTCCTCCGGTAGTCATCTCGTCACTTTGCATTTTCAACCTCATCCTTTCTTTTCATGT
GTTACCGACGCCGTAAACGGTGCGCGTAGCCGTTTTTCGGCATTTTATCCAAAAGCAAAC
TATGGCTTACAAGGAAGCCAACCCTCTGATGTTCGTGCGCATAATCGCGCTGCCAACGGC
GCGTGTGATGAATACAAACAACTCAAGGTGCTCTCCATGGGAAGACAAAAAGCAGTGATC
AAAGCTCGTCGCGAGGCAAAACGTGTGCTGAGACGGGATTCGCGCAGCCATAAGCAGCGT
GAAGAAGAATCGGTCACCTCGCTTGTGCAGATGGGCGGCGTAGAAGCCATTGGTATGGCC
CGCGACAGTCGCGATACTTCGCCCATCCTCGCGCGAAATGAAGCGCAATTGCACTATCTG
AAGGCTATTGAGAGTAAGCAGCTGATATTCGCCACGGGCGAAGCCGGGTGCGGAAAAACC
TGGATCAGCGCAGCAAAAGCGGCAGAGGCCCTGATACATAAGGATGTCGACAGGATTATC
GTCACCCGTCCAGTTCTGCAAGCCGATGAAGATCTTGGCTTCTTACCTGGAGATATCGCA
GAAAAGTTTGCTCCCTATTTTCGCCCGGTCTACGACGTGCTGGTCCGGCGCTTAGGGGCT
TCCTTTATGCAGTACTGCCTGCGACCGGAAATAGGGAAGGTGGAAATTGCGCCGTTCGCC
TATATGCGTGGACGTACCTTTGAAAATGCAGTCGTCATTCTTGACGAGGCGCAGAATGTG
ACTGCCGCGCAAATGAAAATGTTTTTAACCCGCCTCGGGGAGAACGTGACGGTTATCGTC
AACGGTGATATCACGCAATGCGATTTGCCTCGCGGCGTGTGCTCCGGATTAAGTGACGCG
CTGGAACGTTTCGAAGAAGATGAAATGGTTGGGATCGTCCGGTTCGGTAAAGAGGACTGC
GTACGTTCGGCACTTTGCCAACGTACGCTGCATGCCTACAGTTAA
Protein Properties
Pfam Domain Function:
Protein Residues:354
Protein Molecular Weight:39248
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Uroporphyrinogen decarboxylase
MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAGDFMSLCKNAELACEVT
LQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSPVTCKADVDKLPIPDPEDE
LGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGSSKAFTVIKKMMYADPQALHA
LLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRDYQQFSLYYMHKIVDGLLRENDG
RRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIADARRRVGNKVALQGNMDPSMLYAP
PARIEEEVATILAGFGHGEGHVFNLGHGIHQDVPPEHAGVFVEAVHRLSEQYHR
References
External Links:
ResourceLink
Uniprot ID:P29680
Uniprot Name:DCUP_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1651507
Ecogene ID:EG11543
Ecocyc:EG11543
ColiBase:b3997
Kegg Gene:b3997
EchoBASE ID:EB1505
CCDB:DCUP_ECOLI
BacMap:16131827
General Reference:
  • Blattner, F. R., Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L. (1993). "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Nucleic Acids Res 21:5408-5417. Pubmed: 8265357
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Nishimura, K., Nakayashiki, T., Inokuchi, H. (1993). "Cloning and sequencing of the hemE gene encoding uroporphyrinogen III decarboxylase (UPD) from Escherichia coli K-12." Gene 133:109-113. Pubmed: 8224882