Probable UDP-N-acetyl-D-mannosaminuronic acid transferase (P27836)

Identification
Name:Probable UDP-N-acetyl-D-mannosaminuronic acid transferase
Synonyms:
  • UDP-ManNAcA transferase
Gene Name:wecG
Enzyme Class:
Biological Properties
General Function:Involved in transferase activity, transferring hexosyl groups
Specific Function:Catalyzes the synthesis of Und-PP-GlcNAc-ManNAcA (Lipid II), the second lipid-linked intermediate involved in ECA synthesis
Cellular Location:Not Available
SMPDB Pathways:
  • Secondary Metabolites: enterobacterial common antigen biosynthesis PW000959
  • Secondary Metabolites: enterobacterial common antigen biosynthesis 2 PW002045
  • Secondary Metabolites: enterobacterial common antigen biosynthesis 3 PW002046
KEGG Pathways:Not Available
KEGG Reactions:
1.0Thumb ↔ 1.0Thumb
1.0N-Acetyl-D-glucosaminyldiphospho-di-trans,octa-cis-undecaprenol ↔ 1.0Uridine 5'-diphosphate
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0UDP-ManNAcA → 1.0Uridine 5'-diphosphate+1.0Thumb+1.0Undecaprenyl N-acetyl-glucosaminyl-N-acetyl-mannosaminuronate pyrophosphate+1.0Thumb+1.0Thumb
1.0N-acetyl-α-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenol + 1.0UDP-ManNAcA → 1.0Uridine 5'-diphosphate + 1.0Hydrogen ion + 1.0Undecaprenyl N-acetyl-glucosaminyl-N-acetyl-mannosaminuronate pyrophosphate + 1.0Uridine 5'-diphosphate + 1.0Undecaprenyl-diphospho-N-acetylglucosamine-N-acetylmannosaminuronate
ReactionCard
1.0Thumb+1.0UDP-ManNAcA → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Undecaprenyl-N-acetyl-alpha-D-glucosaminyl-pyrophosphate + 1.0UDP-ManNAcA → 1.0Uridine 5'-diphosphate + 1.0Undecaprenyl-diphospho-N-acetylglucosamine-N-acetylmannosaminuronate + 1.0Hydrogen ion
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb+1.0Thumb
1.0Undecaprenyl-N-acetyl-alpha-D-glucosaminyl-pyrophosphate + 1.0UDP-N-Acetyl-D-mannosaminouronate ↔ 1.0Hydrogen ion + 1.0Undecaprenyl phosphate + 1.0Uridine 5'-diphosphate
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0UDP-N-Acetyl-D-mannosaminouronate + 1.0Undecaprenyl-N-acetyl-alpha-D-glucosaminyl-pyrophosphate → 1.0Hydrogen ion + 1.0Uridine 5'-diphosphate + 1.0Undecaprenyl-diphospho-N-acetylglucosamine-N-acetylmannosaminuronate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB21225Hydrogen ionMetaboCard
ECMDB24257N-acetyl-α-D-glucosaminyl-diphospho-ditrans,octacis-undecaprenolMetaboCard
ECMDB21294UDP-N-Acetyl-D-mannosaminouronateMetaboCard
ECMDB20208Undecaprenyl phosphateMetaboCard
ECMDB21298Undecaprenyl-diphospho-N-acetylglucosamine-N-acetylmannosaminuronateMetaboCard
ECMDB20560Undecaprenyl-N-acetyl-alpha-D-glucosaminyl-pyrophosphateMetaboCard
ECMDB00295Uridine 5'-diphosphateMetaboCard
GO Classification:
Process
biosynthetic process
metabolic process
Gene Properties
Blattner:b3794
Gene OrientationClockwise
Centisome Percentage:85.74
Left Sequence End3977979
Right Sequence End3978719
Gene Sequence:
>741 bp
ATGAGTATCCTTGTCACCCGCCCGTCTCCCGCTGGAGAAGAGTTAGTGAGCCGTCTGCGC
ACACTGGGGCAGGTGGCCTGGCATTTTCCGCTGATTGAGTTTTCTCCGGGTCAACAATTA
CCGCAACTTGCTGATCAACTGGCAGCGCTGGGGGAGAGCGATCTGTTGTTTGCCCTCTCG
CAACACGCGGTTGCTTTTGCCCAATCACAGCTGCATCAGCAAGATCGTAAATGGCCCCGA
CTACCTGATTATTTCGCCATTGGACGCACCACCGCACTGGCACTACATACCGTAAGTGGA
CAGAAGATTCTCTACCCGCAGGATCGGGAAATCAGCGAAGTCTTGCTACAATTACCTGAA
TTACAAAATATTGCGGGCAAACGTGCGCTGATATTACGTGGCAATGGTGGTCGTGAGCTA
ATTGGGGATACCCTGACGGCGCGCGGTGCTGAGGTCACTTTTTGTGAATGTTATCAACGA
TGCGCAATCCATTACGATGGTGCAGAAGAAGCGATGCGCTGGCAAGCCCGCGAGGTGACG
ATGGTCGTTGTTACCAGCGGTGAAATGTTGCAGCAACTCTGGTCGCTGATCCCACAATGG
TATCGTGAGCACTGGTTACTACACTGTCGACTATTGGTCGTCAGTGAGCGTTTGGCGAAA
CTCGCCCGGGAACTGGGCTGGCAAGACATTAAGGTCGCCGATAACGCTGACAACGATGCG
CTTTTACGGGCATTACAATAA
Protein Properties
Pfam Domain Function:
Protein Residues:246
Protein Molecular Weight:27928
Protein Theoretical pI:10
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Probable UDP-N-acetyl-D-mannosaminuronic acid transferase
MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAINAEKMLTIEDNAEVRE
LINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMARAGKEGTPVFLVGGKPEV
LAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASGAQIVTVAMGSPKQEIIMRDC
RLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWLYRLLSQPSRIKRQLRLLRYLRW
HYTGNL
References
External Links:
ResourceLink
Uniprot ID:P27836
Uniprot Name:WECG_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85676247
Ecogene ID:EG11458
Ecocyc:EG11458
ColiBase:b3794
Kegg Gene:b3794
EchoBASE ID:EB1427
CCDB:WECG_ECOLI
BacMap:16131650
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Daniels, D. L., Plunkett, G. 3rd, Burland, V., Blattner, F. R. (1992). "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes." Science 257:771-778. Pubmed: 1379743
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553