Putative ECA polymerase (P27835)

Identification
Name:Putative ECA polymerase
Synonyms:Not Available
Gene Name:wzyE
Enzyme Class:Not Available
Biological Properties
General Function:Not Available
Specific Function:Could be involved in ECA polysaccharide chain elongation
Cellular Location:Cell membrane; Multi-pass membrane protein (Potential)
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
Complex Reactions:
1.0(enterobacterial common antigen)x2 undecaprenyl-diphosphate+1.0Thumb → 1.0(enterobacterial common antigen)x3 undecaprenyl-diphosphate+1.0Thumb+1.0Thumb
1.0(enterobacterial common antigen)x2 undecaprenyl-diphosphate + 1.0Undecaprenyl-diphospho N-acetylglucosamine-N-acetylmannosaminuronate-N-acetamido-4,6-dideoxy-D-galactose → 1.0(enterobacterial common antigen)x3 undecaprenyl-diphosphate + 1.0Hydrogen ion + 1.0Undecaprenyl diphosphate
ReactionCard
1.0(enterobacterial common antigen)x3 undecaprenyl-diphosphate+1.0Thumb → 1.0(enterobacterial common antigen)x4 undecaprenyl-diphosphate+1.0Thumb+1.0Thumb
1.0(enterobacterial common antigen)x3 undecaprenyl-diphosphate + 1.0Undecaprenyl-diphospho N-acetylglucosamine-N-acetylmannosaminuronate-N-acetamido-4,6-dideoxy-D-galactose → 1.0(enterobacterial common antigen)x4 undecaprenyl-diphosphate + 1.0Hydrogen ion + 1.0Undecaprenyl diphosphate
ReactionCard
2.0Thumb → 1.0(enterobacterial common antigen)x2 undecaprenyl-diphosphate+1.0Thumb+1.0Thumb
2.0Undecaprenyl-diphospho N-acetylglucosamine-N-acetylmannosaminuronate-N-acetamido-4,6-dideoxy-D-galactose → 1.0(enterobacterial common antigen)x2 undecaprenyl-diphosphate + 1.0Hydrogen ion + 1.0Undecaprenyl diphosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB21225Hydrogen ionMetaboCard
ECMDB01469Undecaprenyl diphosphateMetaboCard
ECMDB21297Undecaprenyl-diphospho N-acetylglucosamine-N-acetylmannosaminuronate-N-acetamido-4,6-dideoxy-D-galactoseMetaboCard
GO Classification:
Component
cell part
integral to membrane
intrinsic to membrane
membrane part
Gene Properties
Blattner:b3793
Gene OrientationClockwise
Centisome Percentage:85.71
Left Sequence End3976624
Right Sequence End3977976
Gene Sequence:
>1353 bp
ATGAGTCTGCTGCAATTCAGTGGCCTGTTTGTTGTCTGGCTGCTCTGCACGCTGTTTATT
GCCACGCTGACCTGGTTTGAGTTTCGCCGTGTGCGCTTTAACTTCAATGTCTTCTTTTCA
TTGCTGTTTTTGCTCACCTTTTTCTTCGGCTTCCCGCTGACCAGCGTGCTGGTATTTCGC
TTTGATGTTGGTGTCGCGCCGCCAGAAATCTTGTTGCAGGCGTTGCTTTCTGCGGGCTGC
TTCTACGCGGTTTACTATGTCACCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTA
CCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGAGACCAATCTTACGTGGGTGATCCTG
ATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTC
CGGCTTAACTCCTACAGTCAGATCTTTTCCAGTGAAGTCTCCGGCGTGGCGTTAAAACGC
TTCTTTTACTTTTTCATCCCGGCGATGCTGGTGGTCTACTTTCTGCGCCAGGACAGCAAA
GCGTGGCTGTTTTTCCTCGTCAGCACGGTCGCCTTTGGCTTGCTGACTTATATGATTGTC
GGCGGCACTCGCGCCAATATCATCATCGCATTCGCTATCTTCCTGTTTATTGGCATTATT
CGCGGCTGGATTTCGTTGTGGATGCTGGCGGCGGCGGGCGTGCTGGGGATTGTTGGCATG
TTCTGGCTGGCACTAAAACGCTATGGAATGAATGTGAGCGGCGATGAAGCGTTCTATACG
TTTCTCTATCTCACTCGCGACACCTTCTCGCCGTGGGAGAATCTGGCGTTGCTGTTGCAG
AACTACCACAACATCGACTTCCAGGGCCTGGCTCCAATTGTCCGCGATTTCTATGTCTTT
ATCCCTTCCTGGCTGTGGCCGGGTCGCCCGAGTATGGTGCTGAACTCAGCCAACTACTTT
ACCTGGGAAGTGCTGAATAACCACTCCGGACTGGCGATCTCGCCTACGCTTATAGGCTCA
CTGGTGGTGATGGGCGGCGCGTTGTTCATCCCGCTCGGGGCGATCGTGGTTGGTCTGATC
ATCAAATGGTTCGACTGGCTGTATGAGCTGGGCAACCGCGAGCCTAATCGCTATAAAGCT
GCGATATTGCACAGTTTCTGCTTTGGGGCGATCTTCAATATGATCGTGCTGGCGCGTGAA
GGGCTGGATTCGTTTGTCTCACGCGTGGTCTTTTTTATCGTGGTCTTCGGCGCATGTCTG
ATGATCGCAAAACTGTTGTACTGGCTTTTTGAAAGCGCCGGACTCATTCATAAACGTACA
AAATCATCGCTCCGGACGCAGGTTGAAGGATAA
Protein Properties
Pfam Domain Function:
Protein Residues:450
Protein Molecular Weight:51517
Protein Theoretical pI:10
Signaling Regions:
  • None
Transmembrane Regions:
  • 6-26
  • 37-57
  • 63-83
  • 118-138
  • 155-175
  • 181-201
  • 207-227
  • 228-248
  • 341-361
  • 378-398
  • 410-430
Protein Sequence:
>Putative ECA polymerase
MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFLLTFFFGFPLTSVLVFR
FDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPRRPLFTMNRVETNLTWVIL
MGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKRFFYFFIPAMLVVYFLRQDSK
AWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIGIIRGWISLWMLAAAGVLGIVGM
FWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLALLLQNYDNIDFQGLAPIVRDFYVF
IPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAISPTLIGSLVVMGGALFIPLGAIVVGLI
IKWFDWLYELGNREPNRYKAAILHSFCFGAIFNMIVLAREGLDSFVSRVVFFIVVFGACL
MIAKLLYWLFESAGLIHKRTKSSLRTQVEG
References
External Links:
ResourceLink
Uniprot ID:P27835
Uniprot Name:WZYE_ECOLI
GenBank Gene ID:AF375882
Genebank Protein ID:14485184
Ecogene ID:EG11457
Ecocyc:EG11457
ColiBase:b3793
Kegg Gene:b3793
EchoBASE ID:EB1426
CCDB:WZYE_ECOLI
BacMap:16131649
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Daniels, D. L., Plunkett, G. 3rd, Burland, V., Blattner, F. R. (1992). "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes." Science 257:771-778. Pubmed: 1379743
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Plunkett, G. 3rd, Burland, V., Daniels, D. L., Blattner, F. R. (1993). "Analysis of the Escherichia coli genome. III. DNA sequence of the region from 87.2 to 89.2 minutes." Nucleic Acids Res 21:3391-3398. Pubmed: 8346018
  • Rahman, A., Barr, K., Rick, P. D. (2001). "Identification of the structural gene for the TDP-Fuc4NAc:lipid II Fuc4NAc transferase involved in synthesis of enterobacterial common antigen in Escherichia coli K-12." J Bacteriol 183:6509-6516. Pubmed: 11673418