Lipopolysaccharide biosynthesis protein rffA (P27833)

Identification
Name:Lipopolysaccharide biosynthesis protein rffA
Synonyms:Not Available
Gene Name:rffA
Enzyme Class:
Biological Properties
General Function:Involved in catalytic activity
Specific Function:Involved in ECA elongation
Cellular Location:Not Available
SMPDB Pathways:
  • Secondary Metabolites: enterobacterial common antigen biosynthesis PW000959
  • Secondary Metabolites: enterobacterial common antigen biosynthesis 2 PW002045
  • Secondary Metabolites: enterobacterial common antigen biosynthesis 3 PW002046
KEGG Pathways:Not Available
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0dTDP-D-Fucosamine + 1.0alpha-Ketoglutarate ↔ 1.04,6-Dideoxy-4-oxo-dTDP-D-glucose + 1.0L-Glutamate
ReactionCard
SMPDB Reactions:
1.0L-Glutamic acid+1.0dTDP-4-dehydro-6-deoxy-D-glucose+1.0Thumb → 1.0Thumb+1.0Thumb
1.0L-Glutamic acid + 1.0dTDP-4-dehydro-6-deoxy-D-glucose + 1.0L-Glutamate → 1.0Oxoglutaric acid + 1.0 dTDP-thomosamine
ReactionCard
1.0dTDP-4-dehydro-6-deoxy-D-glucose+1.0Thumb → 1.0Thumb+1.0Thumb
1.0dTDP-4-dehydro-6-deoxy-D-glucose + 1.0L-Glutamate → 1.0Oxoglutaric acid + 1.0dTDP-D-Fucosamine
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.04,6-Dideoxy-4-oxo-dTDP-D-glucose + 1.0L-Glutamate ↔ 1.0dTDP-D-Fucosamine + 1.0Oxoglutaric acid
ReactionCard
Complex Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.04,6-Dideoxy-4-oxo-dTDP-D-glucose + 1.0L-Glutamate → 1.0alpha-Ketoglutarate + 1.0dTDP-D-Fucosamine
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB24258 dTDP-thomosamineMetaboCard
ECMDB013994,6-Dideoxy-4-oxo-dTDP-D-glucoseMetaboCard
ECMDB02812alpha-KetoglutarateMetaboCard
ECMDB20612dTDP-D-FucosamineMetaboCard
ECMDB00148L-GlutamateMetaboCard
ECMDB04123Oxoglutaric acidMetaboCard
GO Classification:
Function
binding
catalytic activity
cofactor binding
pyridoxal phosphate binding
Gene Properties
Blattner:b3791
Gene OrientationClockwise
Centisome Percentage:85.63
Left Sequence End3973169
Right Sequence End3974299
Gene Sequence:
>1131 bp
ATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAA
ATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGAC
AAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCG
CAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGC
GGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGAT
ATTTTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAAC
ATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACT
CTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACT
TGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGCCAGGGATTCTTCGCTGTACAG
CAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGT
CATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTG
GACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCG
GCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGC
AACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATC
GAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGT
AAGCTATTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGAT
TTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAAAGGCAGAAACAGCTG
CTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCA
AAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAA
Protein Properties
Pfam Domain Function:
Protein Residues:376
Protein Molecular Weight:41901
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Lipopolysaccharide biosynthesis protein rffA
MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRFGSAKVLLTPSCTASLE
MAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRPDTMNIDETLIEAAITDKT
RVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMSTYKGRALGTIGHIGCFSFHET
KNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFRGQVDKYTWRDIGSSYLMSDLQA
AYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGRIELPSIPDGCVQNAHMFYIKLRDI
DDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFGEFHGEDRYTTKESERLLRLPLFYNLS
PVNQRTVIATLLNYFS
References
External Links:
ResourceLink
Uniprot ID:P27833
Uniprot Name:RFFA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:21321903
Ecogene ID:EG11456
Ecocyc:EG11456
ColiBase:b3791
Kegg Gene:b3791
EchoBASE ID:EB1425
CCDB:RFFA_ECOLI
BacMap:16131647
General Reference:
  • Barr, K., Rick, P. D. (1993). "Physical map location of the rffC and rffA genes of Escherichia coli." J Bacteriol 175:5738-5739. Pubmed: 8366065
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Daniels, D. L., Plunkett, G. 3rd, Burland, V., Blattner, F. R. (1992). "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes." Science 257:771-778. Pubmed: 1379743
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553