UDP-N-acetylglucosamine 2-epimerase (P27828)

Identification
Name:UDP-N-acetylglucosamine 2-epimerase
Synonyms:
  • Bacteriophage N4 adsorption protein C
  • UDP-GlcNAc-2-epimerase
Gene Name:wecB
Enzyme Class:
Biological Properties
General Function:Involved in UDP-N-acetylglucosamine 2-epimerase activity
Specific Function:Catalyzes the reversible epimerization at C-2 of UDP-N- acetylglucosamine (UDP-GlcNAc) and thereby provides bacteria with UDP-N-acetylmannosamine (UDP-ManNAc), the activated donor of ManNAc residues. Also involved in bacteriophage N4 adsorption
Cellular Location:Cytoplasm
SMPDB Pathways:
  • Amino sugar and nucleotide sugar metabolism I PW000886
KEGG Pathways:
KEGG Reactions:
1.0Thumb ↔ 1.0Thumb
1.0Uridine diphosphate-N-acetylglucosamine ↔ 1.0UDP-N-Acetyl-D-mannosamine
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Uridine diphosphate-N-acetylglucosamine + 1.0Water ↔ 1.0N-Acetylmannosamine + 1.0Uridine 5'-diphosphate
ReactionCard
SMPDB Reactions:
1.0Thumb ↔ 1.0UDP-N-acetyl-D-mannosamine+1.0Thumb
1.0Uridine diphosphate-N-acetylglucosamine ↔ 1.0UDP-N-acetyl-D-mannosamine + 1.0UDP-N-Acetyl-D-mannosamine
ReactionCard
EcoCyc Reactions:
1.0Thumb ↔ 1.0Thumb
1.0Uridine diphosphate-N-acetylglucosamine ↔ 1.0UDP-N-Acetyl-D-mannosamine
ReactionCard
Complex Reactions:
1.0Thumb → 1.0Thumb
1.0Uridine diphosphate-N-acetylglucosamine → 1.0UDP-N-Acetyl-D-mannosamine
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB01129N-AcetylmannosamineMetaboCard
ECMDB20202UDP-N-Acetyl-D-mannosamineMetaboCard
ECMDB00295Uridine 5'-diphosphateMetaboCard
ECMDB00290Uridine diphosphate-N-acetylglucosamineMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
catalytic activity
isomerase activity
racemase and epimerase activity
racemase and epimerase activity, acting on carbohydrates and derivatives
UDP-N-acetylglucosamine 2-epimerase activity
Process
alcohol metabolic process
amino sugar metabolic process
glucosamine metabolic process
lipid biosynthetic process
lipid metabolic process
lipopolysaccharide biosynthetic process
metabolic process
monosaccharide metabolic process
N-acetylglucosamine metabolic process
primary metabolic process
small molecule metabolic process
UDP-N-acetylglucosamine metabolic process
Gene Properties
Blattner:b3786
Gene OrientationClockwise
Centisome Percentage:85.53
Left Sequence End3968156
Right Sequence End3969286
Gene Sequence:
>1131 bp
ATGGCTAAGCAAGATTATTACGAGATTTTAGGCGTTTCCAAAACAGCGGAAGAGCGTGAA
ATCAGAAAGGCCTACAAACGCCTGGCCATGAAATACCACCCGGACCGTAACCAGGGTGAC
AAAGAGGCCGAGGCGAAATTTAAAGAGATCAAGGAAGCTTATGAAGTTCTGACCGACTCG
CAAAAACGTGCGGCATACGATCAGTATGGTCATGCTGCGTTTGAGCAAGGTGGCATGGGC
GGCGGCGGTTTTGGCGGCGGCGCAGACTTCAGCGATATTTTTGGTGACGTTTTCGGCGAT
ATTTTTGGCGGCGGACGTGGTCGTCAACGTGCGGCGCGCGGTGCTGATTTACGCTATAAC
ATGGAGCTCACCCTCGAAGAAGCTGTACGTGGCGTGACCAAAGAGATCCGCATTCCGACT
CTGGAAGAGTGTGACGTTTGCCACGGTAGCGGTGCAAAACCAGGTACACAGCCGCAGACT
TGTCCGACCTGTCATGGTTCTGGTCAGGTGCAGATGCGCCAGGGATTCTTCGCTGTACAG
CAGACCTGTCCACACTGTCAGGGCCGCGGTACGCTGATCAAAGATCCGTGCAACAAATGT
CATGGTCATGGTCGTGTTGAGCGCAGCAAAACGCTGTCCGTTAAAATCCCGGCAGGGGTG
GACACTGGAGACCGCATCCGTCTTGCGGGCGAAGGTGAAGCGGGCGAGCATGGCGCACCG
GCAGGCGATCTGTACGTTCAGGTTCAGGTTAAACAGCACCCGATTTTCGAGCGTGAAGGC
AACAACCTGTATTGCGAAGTCCCGATCAACTTCGCTATGGCGGCGCTGGGTGGCGAAATC
GAAGTACCGACCCTTGATGGTCGCGTCAAACTGAAAGTGCCTGGCGAAACCCAGACCGGT
AAGCTATTCCGTATGCGCGGTAAAGGCGTCAAGTCTGTCCGCGGTGGCGCACAGGGTGAT
TTGCTGTGCCGCGTTGTCGTCGAAACACCGGTAGGCCTGAACGAAAGGCAGAAACAGCTG
CTGCAAGAGCTGCAAGAAAGCTTCGGTGGCCCAACCGGCGAGCACAACAGCCCGCGCTCA
AAGAGCTTCTTTGATGGTGTGAAGAAGTTTTTTGACGACCTGACCCGCTAA
Protein Properties
Pfam Domain Function:
Protein Residues:376
Protein Molecular Weight:42244
Protein Theoretical pI:6
PDB File:1VGV
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>UDP-N-acetylglucosamine 2-epimerase
MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLDQVLKLFSIVPDYDLNI
MQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLATSLAAFYQRIPVGHVEAGL
RTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLRENVADSRIFITGNTVIDALLWV
RDQVMSSDKLRSELAANYPFIDPDKKMILVTGHRRESFGRGFEEICHALADIATTHQDIQ
IVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPFVWLMNHAWLILTDSGGIQEEAPSL
GKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVEEVTRLLKDENEYQAMSRAHNPYGDGQ
ACSRILEALKNNRISL
References
External Links:
ResourceLink
Uniprot ID:P27828
Uniprot Name:WECB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:21321903
PDB ID:1VGV
Ecogene ID:EG11451
Ecocyc:EG11451
ColiBase:b3786
Kegg Gene:b3786
EchoBASE ID:EB1420
CCDB:WECB_ECOLI
BacMap:49176409
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Campbell, R. E., Mosimann, S. C., Tanner, M. E., Strynadka, N. C. (2000). "The structure of UDP-N-acetylglucosamine 2-epimerase reveals homology to phosphoglycosyl transferases." Biochemistry 39:14993-15001. Pubmed: 11106477
  • Daniels, D. L., Plunkett, G. 3rd, Burland, V., Blattner, F. R. (1992). "Analysis of the Escherichia coli genome: DNA sequence of the region from 84.5 to 86.5 minutes." Science 257:771-778. Pubmed: 1379743
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Kiino, D. R., Licudine, R., Wilt, K., Yang, D. H., Rothman-Denes, L. B. (1993). "A cytoplasmic protein, NfrC, is required for bacteriophage N4 adsorption." J Bacteriol 175:7074-7080. Pubmed: 8226648
  • Macpherson, D. F., Manning, P. A., Morona, R. (1994). "Characterization of the dTDP-rhamnose biosynthetic genes encoded in the rfb locus of Shigella flexneri." Mol Microbiol 11:281-292. Pubmed: 8170390
  • Marolda, C. L., Valvano, M. A. (1995). "Genetic analysis of the dTDP-rhamnose biosynthesis region of the Escherichia coli VW187 (O7:K1) rfb gene cluster: identification of functional homologs of rfbB and rfbA in the rff cluster and correct location of the rffE gene." J Bacteriol 177:5539-5546. Pubmed: 7559340
  • Riley, M., Abe, T., Arnaud, M. B., Berlyn, M. K., Blattner, F. R., Chaudhuri, R. R., Glasner, J. D., Horiuchi, T., Keseler, I. M., Kosuge, T., Mori, H., Perna, N. T., Plunkett, G. 3rd, Rudd, K. E., Serres, M. H., Thomas, G. H., Thomson, N. R., Wishart, D., Wanner, B. L. (2006). "Escherichia coli K-12: a cooperatively developed annotation snapshot--2005." Nucleic Acids Res 34:1-9. Pubmed: 16397293