ECMDB

Identification

Name:

O-antigen ligase

Synonyms:

Not Available

Gene Name:

rfaL

Enzyme Class:

Not Available

Biological Properties

General Function:

Cell wall/membrane/envelope biogenesis

Specific Function:

Adds the O-antigen on the glucose group of LPS

Cellular Location:

Cell inner membrane; Multi-pass membrane protein

SMPDB Pathways:

Lipopolysaccharide biosynthesis PW000831

KEGG Pathways:

Lipopolysaccharide biosynthesis ec00540

EcoCyc Reactions:

1.0

1.0colanic acid

→

1.0M_LPS

1.0Lipid A-core + 1.0colanic acid → 1.0M_LPS
ReactionCard

Complex Reactions:

1.0core oligosaccharide lipid A

1.0(enterobacterial common antigen)x4 undecaprenyl-diphosphate

→

1.0(enterobacterial common antigen)x4 core oligosaccharide lipid A

1.0

1.0core oligosaccharide lipid A + 1.0(enterobacterial common antigen)x4 undecaprenyl-diphosphate → 1.0(enterobacterial common antigen)x4 core oligosaccharide lipid A + 1.0Hydrogen ion + 1.0Undecaprenyl diphosphate
ReactionCard

1.0core oligosaccharide lipid A

1.0(O16 antigen)x4 undecaprenyl diphosphate

→

1.0

1.0(O16 antigen)x4 core oligosaccharide lipid A

1.0

1.0core oligosaccharide lipid A + 1.0(O16 antigen)x4 undecaprenyl diphosphate → 1.0Hydrogen ion + 1.0(O16 antigen)x4 core oligosaccharide lipid A + 1.0Undecaprenyl diphosphate
ReactionCard

Metabolites:

ECMDB ID	Name	View
ECMDB21225	Hydrogen ion	MetaboCard
ECMDB20535	Lipid A-core	MetaboCard
ECMDB01469	Undecaprenyl diphosphate	MetaboCard

GO Classification:

Not Available

Gene Properties

Blattner:

b3622

Gene Orientation

Clockwise

Centisome Percentage:

81.79

Left Sequence End

3794971

Right Sequence End

3796230

Gene Sequence:

>1260 bp
GTGTCGCAAGATAACAACTTTAGCCAGGGGCCAGTCCCGCAGTCGGCGCGGAAAGGGGTA
TTGGCATTGACGTTCGTCATGCTGGGATTAACCTTCTTTTCCGCCAGTATGTGGACCGGC
GGCACTCTCGGAACCGGTCTTAGCTATCATGATTTCTTCCTCGCAGTTCTCATCGGTAAT
CTTCTCCTCGGTATTTACACTTCATTTCTCGGTTACATTGGCGCAAAAACCGGCCTGACC
ACTCATCTTCTTGCTCGCTTCTCGTTTGGTGTTAAAGGCTCATGGCTGCCTTCACTGCTA
CTGGGCGGAACTCAGGTTGGCTGGTTTGGCGTCGGTGTGGCGATGTTTGCCATTCCGGTG
GGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACC
GTCACCGTCTTTTTTGGCATTTCGGCGCTGACGGTTCTTTCGGTGATTGCGGTTCCGGCT
ATCGCCTGCCTGGGCGGTTATTCCGTGTGGCTGGCTGTTAACGGCATGGGCGGCCTGGAC
GCATTAAAAGCGGTCGTTCCCGCACAACCGTTAGATTTCAATGTCGCGCTGGCGCTGGTT
GTGGGGTCATTTATCAGTGCGGGTACGCTCACCGCTGACTTTGTCCGGTTTGGTCGCAAT
GCCAAACTGGCGGTGCTGGTGGCGATGGTGGCCTTTTTCCTCGGCAACTCGTTGATGTTT
ATTTTCGGTGCAGCGGGCGCTGCGGCACTGGGCATGGCGGATATCTCTGATGTGATGATT
GCTCAGGGCCTGCTGCTGCCTGCGATTGTGGTGCTGGGGCTGAATATCTGGACCACCAAC
GATAACGCACTCTATGCGTCGGGTTTAGGTTTCGCCAACATTACCGGGATGTCGAGCAAA
ACCCTTTCGGTAATCAACGGTATTATCGGTACGGTCTGCGCATTATGGCTGTATAACAAT
TTTGTCGGCTGGTTGACCTTCCTTTCGGCAGCTATTCCTCCAGTGGGTGGCGTGATCATC
GCCGACTATCTGATGAACCGTCGCCGCTATGAGCACTTTGCGACCACGCGTATGATGAGT
GTCAATTGGGTGGCGATTCTGGCGGTCGCCTTGGGGATTGCTGCAGGCCACTGGTTACCG
GGAATTGTTCCGGTCAACGCGGTATTAGGTGGCGCGCTGAGCTATCTGATCCTTAACCCG
ATTTTGAATCGTAAAACGACAGCAGCAATGACGCATGTGGAGGCTAACAGTGTCGAATAA

Protein Properties

Pfam Domain Function:

Wzy_C (PF04932 )

Protein Residues:

419

Protein Molecular Weight:

46877

Protein Theoretical pI:

Signaling Regions:

None

Transmembrane Regions:

18-38
40-60
72-92
106-126
136-156
169-189
194-214
216-236
242-262
347-367
370-390
391-411

Protein Sequence:

>O-antigen ligase
MLTSFKLHSLKPYTLKSSMILEIITYILCFFSMIIAFVDNTFSIKIYNITAIVCLLSLIL
RGRQENYNIKNLILPLSIFLIGLLDLIWYSAFKVDNSPFRATYHSYLNTAKIFIFGSFIV
FLTLTSQLKSKKESVLYTLYSLSFLIAGYAMYINSIHENDRISFGVGTATGAAYSTMLIG
IVSGVAILYTKKNHPFLFLLNSCAVLYVLALTQTRATLLLFPIICVAALIAYYNKSPKKF
TSSIVLLIAILASIVIIFNKPIQNRYNEALNDLNSYTNANSVTSLGARLAMYEIGLNIFI
KSPFSFRSAESRAESMNLLVAEHNRLRGALEFSNVHLHNEIIEAGSLKGLMGIFSTLFLY
FSLFYIAYKKRALGLLILTLGIVGIGLSDVIIWARSIPIIIISAIVLLLVINNRNNTIN

References

External Links:

Resource	Link
Uniprot ID:	P27243
Uniprot Name:	RFAL_ECOLI
GenBank Gene ID:	AP009048
Genebank Protein ID:	85674478
Ecogene ID:	EG11424
Ecocyc:	EG11424
ColiBase:	b3622
Kegg Gene:	b3622
EchoBASE ID:	EB1394
CCDB:	RFAL_ECOLI
BacMap:	16131493

General Reference:

Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
Klena, J. D., Pradel, E., Schnaitman, C. A. (1992). "Comparison of lipopolysaccharide biosynthesis genes rfaK, rfaL, rfaY, and rfaZ of Escherichia coli K-12 and Salmonella typhimurium." J Bacteriol 174:4746-4752. Pubmed: 1624462
Sofia, H. J., Burland, V., Daniels, D. L., Plunkett, G. 3rd, Blattner, F. R. (1994). "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Nucleic Acids Res 22:2576-2586. Pubmed: 8041620

O-antigen ligase (P27243)