Identification |
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Name: | Lipopolysaccharide 1,2-glucosyltransferase |
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Synonyms: | Not Available |
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Gene Name: | rfaJ |
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Enzyme Class: | |
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Biological Properties |
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General Function: | Involved in transferase activity, transferring glycosyl groups |
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Specific Function: | UDP-glucose + lipopolysaccharide = UDP + D- glucosyl-lipopolysaccharide |
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Cellular Location: | Not Available |
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SMPDB Pathways: | - Lipopolysaccharide biosynthesis PW000831
- lipopolysaccharide biosynthesis II PW001905
- lipopolysaccharide biosynthesis III PW002059
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KEGG Pathways: | |
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KEGG Reactions: | |
1.0 | + | 1.0 | ↔ | 1.0 | + | 1.0D-Glucosyllipopolysaccharide |
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SMPDB Reactions: | |
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EcoCyc Reactions: | |
1.0 | + | 1.0a lipopolysaccharide | ? | 1.0D-glucosyl-lipopolysaccharide | + | 1.0 |
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Complex Reactions: | |
1.0glucosyl-galactosyl-glucosyl-inner core oligosaccharide lipid A | + | 1.0 | → | 1.0glucosyl-glucosyl-galactosyl-glucosyl-inner core oligosaccharide lipid A | + | 1.0 | + | 1.0 |
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1.0 | + | 1.0 | → | 1.0 | + | 1.0D-glucosyl-lipopolysaccharide |
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Metabolites: | ECMDB ID | Name | View |
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ECMDB24226 | galactosyl-(glucosyl)2-(heptosyl)3-Kdo2-lipid A-bisphosphate | MetaboCard | ECMDB24227 | galactosyl-(glucosyl)3-(heptosyl)3-Kdo2-lipid A-bisphosphate | MetaboCard | ECMDB20533 | Galactosyl-glucosyl2-heptosyl3-KDO2-lipid A-bisphosphate | MetaboCard | ECMDB20534 | Galactosyl-glucosyl3-heptosyl3-KDO2-lipid A-bisphosphate | MetaboCard | ECMDB21225 | Hydrogen ion | MetaboCard | ECMDB21088 | LPS (1-O-antigen) | MetaboCard | ECMDB04171 | UDP-Glucose | MetaboCard | ECMDB00295 | Uridine 5'-diphosphate | MetaboCard |
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GO Classification: | Function |
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catalytic activity | lipopolysaccharide 3-alpha-galactosyltransferase activity | transferase activity | transferase activity, transferring glycosyl groups | UDP-galactosyltransferase activity | UDP-glycosyltransferase activity | Process |
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lipid biosynthetic process | lipid metabolic process | lipopolysaccharide biosynthetic process | metabolic process | primary metabolic process |
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Gene Properties |
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Blattner: | b3626 |
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Gene Orientation | Counterclockwise |
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Centisome Percentage: | 81.88 |
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Left Sequence End | 3799006 |
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Right Sequence End | 3800022 |
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Gene Sequence: | >1017 bp
ATGTTTGTCATCTGGAGCCATAGAACAGGGTTCATCATGAGTCATCAACTTACCTTCGCC
GACAGTGAATTCAGCAGTAAGCGCCGTCAGACCAGAAAAGAGATTTTCTTGTCCCGCATG
GAGCAGATTCTGCCATGGCAAAACATGGTGGAAGTCATCGAGCCGTTTTACCCCAAGGCT
GGTAATGGCCGGCGACCTTATCCGCTGGAAACCATGCTACGCATTCACTGCATGCAGCAT
TGGTACAACCTGAGCGATGGCGCGATGGAAGATGCTCTGTACGAAATCGCCTCCATGCGT
CTGTTTGCCCGGTTATCCCTGGATAGCGCCTTGCCGGACCGCACCACCATCATGAATTTC
CGCCACCTGCTGGAGCAGCATCAACTGGCCCGCCAATTGTTCAAGACCATCAATCGCTGG
CTGGCCGAAGCAGGCGTCATGATGACTCAAGGCACCTTGGTCGATGCCACCATCATTGAG
GCACCCAGCTCGACCAAGAACAAAGAGCAGCAACGCGATCCGGAGATGCATCAGACCAAG
AAAGGCAATCAGTGGCACTTTGGCATGAAGGCCCACATTGGTGTCGATGCCAAGAGTGGC
CTGACCCACAGCCTGGTCACCACCGCGGCCAACGAGCATGACCTCAATCAGCTGGGTAAT
CTGCTGCATGGAGAGGAGCAATTTGTCTCAGCCGATGCCGGCTACCAAGGGGCGCCACAG
CGCGAGGAGCTGGCCGAGGTGGATGTGGACTGGCTGATCGCCGAGCGCCCCGGCAAGGTA
AGAACCTTGAAACAGCATCCACGCAAGAACAAAACGGCCATCAACATCGAATACATGAAA
GCCAGCATCCGGGCCAGGGTGGAGCACCCATTTCGCATCATCAAGCGACAGTTCGGCTTC
GTGAAAGCCAGATACAAGGGGTTGCTGAAAAACGATAACCAACTGGCGATGTTATTCACG
CTGGCCAACCTGTTTCGGGCGGACCAAATGATACGTCAGTGGGAGAGATCTCACTAA |
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Protein Properties |
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Pfam Domain Function: | |
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Protein Residues: | 338 |
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Protein Molecular Weight: | 39040 |
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Protein Theoretical pI: | 9 |
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Signaling Regions: | |
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Transmembrane Regions: | |
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Protein Sequence: | >Lipopolysaccharide 1,2-glucosyltransferase
MDSFPAIEIDKVKAWDFRLANINTSECLNVAYGVDANYLDGVGVSITSIVLNNRHINLDF
YIIADVYNDGFFQKIAKLAEQNQLRITLYRINTDKLQCLPCTQVWSRAMYFRLFAFQLLG
LTLDRLLYLDADVVCKGDISQLLHLGLNGAVAAVVKDVEPMQEKAVSRLSDPELLGQYFN
SGVVYLDLKKWADAKLTEKALSILMSKDNVYKYPDQDVMNVLLKGMTLFLPREYNTIYTI
KSELKDKTHQNYKKLITESTLLIHYTGATKPWHKWAIYPSVKYYKIALENSPWKDDSPRD
AKSIIEFKKRYKHLLVQHHYISGIIAGVCYLCRKYYRK |
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References |
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External Links: | |
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General Reference: | - Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
- Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
- Pradel, E., Parker, C. T., Schnaitman, C. A. (1992). "Structures of the rfaB, rfaI, rfaJ, and rfaS genes of Escherichia coli K-12 and their roles in assembly of the lipopolysaccharide core." J Bacteriol 174:4736-4745. Pubmed: 1624461
- Sofia, H. J., Burland, V., Daniels, D. L., Plunkett, G. 3rd, Blattner, F. R. (1994). "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Nucleic Acids Res 22:2576-2586. Pubmed: 8041620
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