Lipopolysaccharide 1,3-galactosyltransferase (P27128)

Identification
Name:Lipopolysaccharide 1,3-galactosyltransferase
Synonyms:
  • Lipopolysaccharide 3-alpha-galactosyltransferase
Gene Name:rfaI
Enzyme Class:
Biological Properties
General Function:Involved in transferase activity, transferring glycosyl groups
Specific Function:UDP-galactose + lipopolysaccharide = UDP + 3- alpha-D-galactosyl-[lipopolysaccharide glucose]
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Thumb ↔ 1.0Thumb+1.03-alpha-D-Galactosyl-[lipopolysaccharide glucose]
1.0Uridine diphosphategalactose + 1.0LPS (1-O-antigen) ↔ 1.0Uridine 5'-diphosphate + 1.03-alpha-D-Galactosyl-[lipopolysaccharide glucose]
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0galactosyl-glucosyl-(heptosyl)3-Kdo2-lipid A-bisphosphate + 1.0UDP-Glucose → 1.0Uridine 5'-diphosphate + 1.0Hydrogen ion + 1.0galactosyl-(glucosyl)2-(heptosyl)3-Kdo2-lipid A-bisphosphate
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0UDP-Glucose + 1.0Galactosyl-glucosyl-heptosyl3-KDO2-lipid A-bisphosphate → 1.0Hydrogen ion + 1.0Galactosyl-glucosyl2-heptosyl3-KDO2-lipid A-bisphosphate + 1.0Uridine 5'-diphosphate
ReactionCard
Complex Reactions:
1.0galactosyl-glucosyl-inner core oligosaccharide lipid A+1.0Thumb → 1.0glucosyl-galactosyl-glucosyl-inner core oligosaccharide lipid A+1.0Thumb+1.0Thumb
1.0galactosyl-glucosyl-inner core oligosaccharide lipid A + 1.0UDP-Glucose → 1.0glucosyl-galactosyl-glucosyl-inner core oligosaccharide lipid A + 1.0Hydrogen ion + 1.0Uridine 5'-diphosphate
ReactionCard
1.0Thumb+1.0Thumb → 1.0Thumb+1.03-alpha-D-galactosyl-[lipopolysaccharide glucose]
1.0Uridine diphosphategalactose + 1.0LPS (1-O-antigen) → 1.0Uridine 5'-diphosphate + 1.03-alpha-D-galactosyl-[lipopolysaccharide glucose]
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB24226galactosyl-(glucosyl)2-(heptosyl)3-Kdo2-lipid A-bisphosphateMetaboCard
ECMDB24225galactosyl-glucosyl-(heptosyl)3-Kdo2-lipid A-bisphosphateMetaboCard
ECMDB20532Galactosyl-glucosyl-heptosyl3-KDO2-lipid A-bisphosphateMetaboCard
ECMDB20533Galactosyl-glucosyl2-heptosyl3-KDO2-lipid A-bisphosphateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB21088LPS (1-O-antigen)MetaboCard
ECMDB04171UDP-GlucoseMetaboCard
ECMDB00295Uridine 5'-diphosphateMetaboCard
ECMDB00302Uridine diphosphategalactoseMetaboCard
GO Classification:
Function
catalytic activity
lipopolysaccharide 3-alpha-galactosyltransferase activity
transferase activity
transferase activity, transferring glycosyl groups
UDP-galactosyltransferase activity
UDP-glycosyltransferase activity
Process
lipid biosynthetic process
lipid metabolic process
lipopolysaccharide biosynthetic process
metabolic process
primary metabolic process
Gene Properties
Blattner:b3627
Gene OrientationCounterclockwise
Centisome Percentage:81.90
Left Sequence End3800062
Right Sequence End3801081
Gene Sequence:
>1020 bp
ATGCAGCAGGTGTTTTTCCAGGAAACTGAGTTTCTTAATTCAGTTATTGATTATGATCAT
AAAGTAGAAACAGAAAATCTTTGTCTGGACATCGCTTATGGAACTGACAAAAACTTTCTA
TTTGGTTGCGGAATTTCGATTGCTTCAATATTAAAATATAATGAAGGAAGTCGATTGTGT
TTCCATATTTTTACTGATTATTTTGGTGATGATGATCGTAAGTATTTTGATGCCCTGGCG
TTGCAGTATAAAACCAGAATTAAAATATATCTGATTAATGGTGATCGACTGCGCTCATTG
CCTAGCACTAAGAATTGGACTCATGCAATATATTTTCGTTTTGTTATTGCAGATTACTTT
ATTAATAAGGCGCCTAAAGTCCTTTATCTGGATGCAGATATCATTTGTCAGGGGACTATT
GAGCCACTAATTAATTTCTCATTTCCTGACGATAAAGTCGCAATGGTTGTTACAGAAGGG
CAAGCTGACTGGTGGGAAAAACGCGCCCATTCGTTAGGTGTTGCGGGAATTGCTAAAGGT
TACTTTAACTCCGGTTTTTTATTGATTAATACTGCCCAATGGGCGGCTCAGCAGGTTTCT
GCACGAGCTATTGCAATGCTAAATGAGCCAGAAATAATCAAAAAAATAACACATCCTGAT
CAGGATGTGTTAAATATGTTGCTGGCGGATAAACTTATTTTCGCTGATATTAAATATAAC
ACCCAGTTCAGCTTAAATTATCAACTCAAAGAAAGCTTTATAAACCCAGTAACCAACGAT
ACTATTTTTATCCATTATATCGGGCCAACCAAGCCCTGGCATGATTGGGCCTGGGATTAT
CCTGTATCACAAGCCTTTATGGAGGCAAAAAATGCTTCGCCATGGAAGAATACGGCGTTG
CTGAAACCGAACAATAGCAATCAATTAAGATATAGCGCAAAACATATGCTTAAAAAGCAT
AGATATCTAAAAGGATTTAGCAACTATCTTTTTTATTTTATTGAAAAGATAAAGCATTAA
Protein Properties
Pfam Domain Function:
Protein Residues:339
Protein Molecular Weight:39423
Protein Theoretical pI:8
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Lipopolysaccharide 1,3-galactosyltransferase
MQQVFFQETEFLNSVIDYDHKVETENLCLDIAYGTDKNFLFGCGISIASILKYNEGSRLC
FHIFTDYFGDDDRKYFDALALQYKTRIKIYLINGDRLRSLPSTKNWTHAIYFRFVIADYF
INKAPKVLYLDADIICQGTIEPLINFSFPDDKVAMVVTEGQADWWEKRAHSLGVAGIAKG
YFNSGFLLINTAQWAAQQVSARAIAMLNEPEIIKKITHPDQDVLNMLLADKLIFADIKYN
TQFSLNYQLKESFINPVTNDTIFIHYIGPTKPWHDWAWDYPVSQAFMEAKNASPWKNTAL
LKPNNSNQLRYSAKHMLKKHRYLKGFSNYLFYFIEKIKH
References
External Links:
ResourceLink
Uniprot ID:P27128
Uniprot Name:RFAI_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85676415
Ecogene ID:EG11352
Ecocyc:EG11352
ColiBase:b3627
Kegg Gene:b3627
EchoBASE ID:EB1327
CCDB:RFAI_ECOLI
BacMap:16131498
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Pradel, E., Parker, C. T., Schnaitman, C. A. (1992). "Structures of the rfaB, rfaI, rfaJ, and rfaS genes of Escherichia coli K-12 and their roles in assembly of the lipopolysaccharide core." J Bacteriol 174:4736-4745. Pubmed: 1624461
  • Sofia, H. J., Burland, V., Daniels, D. L., Plunkett, G. 3rd, Blattner, F. R. (1994). "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Nucleic Acids Res 22:2576-2586. Pubmed: 8041620