Identification
Name:Lipopolysaccharide 1,6-galactosyltransferase
Synonyms:
  • UDP-D-galactose--(Glucosyl)lipopolysaccharide-alpha-1,3-D-galactosyltransferase
Gene Name:rfaB
Enzyme Class:Not Available
Biological Properties
General Function:Involved in biosynthetic process
Specific Function:Adds a galactose goup to a glucose group of LPS
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
  • Lipopolysaccharide biosynthesis ec00540
SMPDB Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
EcoCyc Reactions:
1.0Thumb+1.0Thumb1.0Thumb+1.0Thumb+1.0Thumb
Complex Reactions:
1.0glucosyl-inner core oligosaccharide lipid A+1.0Thumb1.0galactosyl-glucosyl-inner core oligosaccharide lipid A+1.0Thumb+1.0Thumb
1.0glucosyl-inner core oligosaccharide lipid A + 1.0UDP-Glucose → 1.0galactosyl-glucosyl-inner core oligosaccharide lipid A + 1.0Hydrogen ion + 1.0Uridine 5'-diphosphate
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB24225galactosyl-glucosyl-(heptosyl)3-Kdo2-lipid A-bisphosphateMetaboCard
ECMDB20532Galactosyl-glucosyl-heptosyl3-KDO2-lipid A-bisphosphateMetaboCard
ECMDB24223glucosyl-(heptosyl)3-Kdo2-lipid A-bisphosphateMetaboCard
ECMDB20531Glucosyl-heptosyl3-KDO2-lipid A-bisphosphateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB04171UDP-GlucoseMetaboCard
ECMDB24224UDP-α-D-galactoseMetaboCard
ECMDB00295Uridine 5'-diphosphateMetaboCard
ECMDB00302Uridine diphosphategalactoseMetaboCard
GO Classification:
Process
biosynthetic process
metabolic process
Gene Properties
Blattner:b3628
Gene OrientationCounterclockwise
Centisome Percentage:81.93
Left Sequence End3801081
Right Sequence End3802160
Gene Sequence:
>1080 bp
ATGAAAAAATATCTCGCGCTGGCTTTGATTGCGCCGTTGCTCATCTCCTGTTCGACGACC
AAAAAAGGCGATACCTATAACGAAGCCTGGGTCAAAGATACCAACGGTTTTGATATTCTG
ATGGGGCAATTTGCCCACAATATTGAGAACATCTGGGGCTTCAAAGAGGTGGTGATCGCT
GGTCCTAAGGACTACGTGAAATACACCGATCAATATCAGACCCGCAGCCACATCAACTTC
GATGACGGTACGATTACTATCGAAACCATCGCCGGGACAGAACCTGCCGCGCATTTGCGC
CGGGCAATTATCAAAACGTTATTGATGGGTGACGATCCGAGTTCGGTCGATCTCTATTCC
GACGTTGATGATATTACGATTTCGAAAGAACCTTTCCTTTACGGTCAGGTGGTGGACAAC
ACCGGGCAGCCGATTCGCTGGGAAGGTCGCGCAAGCAACTTCGCGGATTATCTGCTGAAA
AACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATG
GTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCA
CGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTT
AACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACT
GCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTG
TTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTT
TATCTCGGCGGAATTGATAACCCAACATCGCGGCGTTATGCCGTCATCACCGCCTATAAC
GGCGGCGCAGGCAGCGTGCTGCGAGTCTTTTCGAATGATAAGATTCAGGCTGCCAATATT
ATTAACACCATGACGCCGGGCGATGTTTATCAGACGCTGACGACCCGCCATCCCTCTGCG
GAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAA
Protein Properties
Pfam Domain Function:
Protein Residues:359
Protein Molecular Weight:40826
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Lipopolysaccharide 1,6-galactosyltransferase
MKIAFIGEAVSGFGGMETVIRDVITTFRQQHIQSEMFFFCRNDKMDKGWLEGIKYSCSFS
NIRLGFLRRAKHIHALSKWLQEYQPDIVICIDVISCLFAAKARKKSGIDMPVFSWPHFSL
DHKKHAEYITCADYHLAISSGIKQQMINRGVAESTINVIFNPVETKDSVIPAPEEGETAT
FIYVGRMKFEGQKRVKDLLDGLSQAKGNWKLHVLGDGSDFEKCQAYGRELNIDDRIVWYG
WQQYPWELVQQDIEKVSALLLTSSFEGFPMTLLEALSWGIPCISADCVSGPADIIQPDVN
GHLYQPGDIAGFVTLLNKYIAGEIHIEHEKIPASIDEFYQSKYYDRLHKVIISAISRRK
References
External Links:
ResourceLink
Uniprot ID:P27127
Uniprot Name:RFAB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85675773
Ecogene ID:EG11351
Ecocyc:EG11351
ColiBase:b3628
Kegg Gene:b3628
EchoBASE ID:EB1326
CCDB:RFAB_ECOLI
BacMap:226524757
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Pradel, E., Parker, C. T., Schnaitman, C. A. (1992). "Structures of the rfaB, rfaI, rfaJ, and rfaS genes of Escherichia coli K-12 and their roles in assembly of the lipopolysaccharide core." J Bacteriol 174:4736-4745. Pubmed: 1624461
  • Sofia, H. J., Burland, V., Daniels, D. L., Plunkett, G. 3rd, Blattner, F. R. (1994). "Analysis of the Escherichia coli genome. V. DNA sequence of the region from 76.0 to 81.5 minutes." Nucleic Acids Res 22:2576-2586. Pubmed: 8041620