Chorismate--pyruvate lyase (P26602)

Identification
Name:Chorismate--pyruvate lyase
Synonyms:
  • CL
  • CPL
Gene Name:ubiC
Enzyme Class:
Biological Properties
General Function:Involved in chorismate lyase activity
Specific Function:Removes the pyruvyl group from chorismate, with concomitant aromatization of the ring, to provide 4- hydroxybenzoate (4HB) for the ubiquinone pathway
Cellular Location:Cytoplasm
SMPDB Pathways:
  • Secondary Metabolites: Ubiquinol biosynthesis PW000981
  • Secondary Metabolites: Ubiquinol biosynthesis 2 PW002036
KEGG Pathways:
  • Metabolic pathways eco01100
  • Ubiquinone and other terpenoid-quinone biosynthesis ec00130
KEGG Reactions:
1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Chorismate ↔ 1.04-Hydroxybenzoic acid + 1.0Pyruvic acid
ReactionCard
1.0Thumb+1.0Thumb ↔ 1.0Thumb
1.04-Hydroxybenzoic acid + 1.0Pyruvic acid ↔ 1.0Chorismate
ReactionCard
1.0Thumb → 1.0Thumb+1.0Thumb
1.0Chorismate → 1.04-Hydroxybenzoic acid + 1.0Pyruvic acid
ReactionCard
EcoCyc Reactions:
1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0Chorismate ↔ 1.04-Hydroxybenzoic acid + 1.0Pyruvic acid
ReactionCard
1.0Thumb → 1.0Thumb+1.0Thumb
1.0Chorismate → 1.04-Hydroxybenzoic acid + 1.0Pyruvic acid
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB005004-Hydroxybenzoic acidMetaboCard
ECMDB12199ChorismateMetaboCard
ECMDB00243Pyruvic acidMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
carbon-carbon lyase activity
catalytic activity
chorismate lyase activity
lyase activity
oxo-acid-lyase activity
Process
cellular metabolic process
coenzyme metabolic process
cofactor metabolic process
metabolic process
oxidoreduction coenzyme metabolic process
ubiquinone biosynthetic process
ubiquinone metabolic process
Gene Properties
Blattner:b4039
Gene OrientationClockwise
Centisome Percentage:91.61
Left Sequence End4250529
Right Sequence End4251026
Gene Sequence:
>498 bp
ATGTCTGAATATCGTCGTTATTACATCAAGGGGGGAACATGGTTTTTCACGGTGAATTTA
CGAAATCGTCGAAGCCAACTTTTGACCACCCAGTACCAGATGCTCCGTCACGCCATTATT
AAAGTTAAGCGAGACAGGCCTTTTGAAATCAACGCCTGGGTCGTTTTGCCAGAGCATATG
CACTGTATCTGGACATTACCTGAAGGCGATGATGATTTTTCCTCGCGCTGGCGGGAAATT
AAAAAGCAATTTACCCATGCTTGTGGATTGAAAAATATCTGGCAACCACGTTTTTGGGAG
CACGCCATCCGCAATACCAAAGATTATAGGCATCATGTTGATTATATTTATATAAATCCA
GTAAAGCATGGTTGGGTAAAGCAAGTGAGTGATTGGCCATTCTCAACGTTCCATCGCGAT
GTCGCGCGAGGGTTATATCCCATCGATTGGGCGGGGGACGTAACGGATTTTAGTGCCGGG
GAGCGTATCATTTCATAA
Protein Properties
Pfam Domain Function:
Protein Residues:165
Protein Molecular Weight:18777
Protein Theoretical pI:8
PDB File:1G1B
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Chorismate--pyruvate lyase
MSHPALTQLRALRYCKEIPALDPQLLDWLLLEDSMTKRFEQQGKTVSVTMIREGFVEQNE
IPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSGPELALQKLGKTPLGRYLF
TSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLPASPLY
References
External Links:
ResourceLink
Uniprot ID:P26602
Uniprot Name:UBIC_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4902964
PDB ID:1G1B
Ecogene ID:EG11369
Ecocyc:EG11369
ColiBase:b4039
Kegg Gene:b4039
EchoBASE ID:EB1343
CCDB:UBIC_ECOLI
BacMap:90111677
General Reference:
  • Blattner, F. R., Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L. (1993). "Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes." Nucleic Acids Res 21:5408-5417. Pubmed: 8265357
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Gallagher, D. T., Mayhew, M., Holden, M. J., Howard, A., Kim, K. J., Vilker, V. L. (2001). "The crystal structure of chorismate lyase shows a new fold and a tightly retained product." Proteins 44:304-311. Pubmed: 11455603
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Holden, M. J., Mayhew, M. P., Gallagher, D. T., Vilker, V. L. (2002). "Chorismate lyase: kinetics and engineering for stability." Biochim Biophys Acta 1594:160-167. Pubmed: 11825618
  • Nichols, B. P., Green, J. M. (1992). "Cloning and sequencing of Escherichia coli ubiC and purification of chorismate lyase." J Bacteriol 174:5309-5316. Pubmed: 1644758
  • Nishimura, K., Nakahigashi, K., Inokuchi, H. (1992). "Location of the ubiA gene on the physical map of Escherichia coli." J Bacteriol 174:5762. Pubmed: 1512213
  • Siebert, M., Bechthold, A., Melzer, M., May, U., Berger, U., Schroder, G., Schroder, J., Severin, K., Heide, L. (1992). "Ubiquinone biosynthesis. Cloning of the genes coding for chorismate pyruvate-lyase and 4-hydroxybenzoate octaprenyl transferase from Escherichia coli." FEBS Lett 307:347-350. Pubmed: 1644192
  • Siebert, M., Severin, K., Heide, L. (1994). "Formation of 4-hydroxybenzoate in Escherichia coli: characterization of the ubiC gene and its encoded enzyme chorismate pyruvate-lyase." Microbiology 140 ( Pt 4):897-904. Pubmed: 8012607
  • Smith, N., Roitberg, A. E., Rivera, E., Howard, A., Holden, M. J., Mayhew, M., Kaistha, S., Gallagher, D. T. (2006). "Structural analysis of ligand binding and catalysis in chorismate lyase." Arch Biochem Biophys 445:72-80. Pubmed: 16343413
  • Stover, C., Mayhew, M. P., Holden, M. J., Howard, A., Gallagher, D. T. (2000). "Crystallization and 1.1-A diffraction of chorismate lyase from Escherichia coli." J Struct Biol 129:96-99. Pubmed: 10675300
  • Wu, G., Williams, H. D., Gibson, F., Poole, R. K. (1993). "Mutants of Escherichia coli affected in respiration: the cloning and nucleotide sequence of ubiA, encoding the membrane-bound p-hydroxybenzoate:octaprenyltransferase." J Gen Microbiol 139:1795-1805. Pubmed: 8409922