DNA ligase B (P25772)

Identification
Name:DNA ligase B
Synonyms:
  • Polydeoxyribonucleotide synthase [NAD+] B
Gene Name:ligB
Enzyme Class:
Biological Properties
General Function:Involved in DNA ligase (NAD+) activity
Specific Function:Catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction
Cellular Location:Not Available
SMPDB Pathways:Not Available
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0DNA ↔ 1.0Thumb+1.0Thumb
1.0NAD + 1.0DNA ↔ 1.0Adenosine monophosphate + 1.0NMN
ReactionCard
EcoCyc Reactions:
1.0DNAn+1.0(deoxynucleotides)(m)+1.0Thumb → 1.0(deoxynucleotides)(m)+1.0Thumb+1.0Thumb
1.0DNAn + 1.0(deoxynucleotides)(m) + 1.0NAD → 1.0(deoxynucleotides)(m) + 1.0Nicotinamide ribotide + 1.0Adenosine monophosphate
ReactionCard
Complex Reactions:
1.0Thumb+1.0(deoxyribonucleotide)(n)+1.0(deoxyribonucleotide)(m) → 1.0Thumb+1.0Thumb+1.0(deoxyribonucleotide)(n+m)
1.0NAD + 1.0(deoxyribonucleotide)(n) + 1.0(deoxyribonucleotide)(m) → 1.0Adenosine monophosphate + 1.0NMN + 1.0(deoxyribonucleotide)(n+m)
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB00045Adenosine monophosphateMetaboCard
ECMDB00902NADMetaboCard
ECMDB00229Nicotinamide ribotideMetaboCard
ECMDB23222NMNMetaboCard
GO Classification:
Function
catalytic activity
DNA ligase (NAD+) activity
DNA ligase activity
ligase activity
ligase activity, forming phosphoric ester bonds
Process
cellular macromolecule metabolic process
DNA metabolic process
DNA repair
DNA replication
macromolecule metabolic process
metabolic process
Gene Properties
Blattner:b3647
Gene OrientationCounterclockwise
Centisome Percentage:82.28
Left Sequence End3817511
Right Sequence End3819193
Gene Sequence:
>1683 bp
ATGAAAGTATGGATGGCGATATTAATAGGTATCTTGTGCTGGCAATCATCTGTGTGGGCG
GTCTGTCCGGCCTGGTCGCCAGCCAGAGCACAGGAAGAAATTTCCCGCCTGCAACAGCAA
ATAAAACAGTGGGACGATGACTACTGGAAGGAAGGAAAAAGTGAGGTGGAAGACGGTGTT
TACGATCAGTTAAGCGCCCGTCTTACGCAGTGGCAACGCTGCTTTGGGAGCGAGCCCCGC
GACGTGATGATGCCGCCTTTAAATGGTGCGGTTATGCATCCGGTTGCCCATACGGGTGTA
CGCAAAATGGTGGATAAAAACGCATTAAGTCTGTGGATGCGAGAACGTAGCGATCTTTGG
GTGCAGCCAAAAGTTGATGGCGTTGCGGTAACCCTGGTTTATCGGGACGGGAAACTGAAC
AAAGCAATCAGTCGCGGTAATGGCCTGAAAGGCGAGGACTGGACGCAGAAAGTTAGCTTA
ATTTCCGCTGTGCCGCAAACCGTTAGCGGGCCTTTAGCCAACAGTACGCTTCAGGGGGAA
ATATTTCTCCAGCGCGAGGGGCATATCCAACAACAAATGGGGGGAATAAATGCCCGCGCA
AAAGTTGCTGGCTTGATGATGCGCCAGGACGATAGCGACACGCTGAATTCTCTGGGCGTT
TTTGTCTGGGCATGGCCGGATGGACCGCAGTTAATGTCTGATCGTTTAAAAGAGCTGGCT
ACCGCAGGTTTTACTCTAACGCAGACGTATACCCGTGCGGTGAAAAATGCTGATGAAGTT
GCGCGCGTACGCAATGAGTGGTGGAAAGCGGAATTACCCTTCGTCACCGATGGCGTAGTT
GTACGAGCGGCGAAAGAGCCAGAATCCCGCCATTGGCTACCGGGCCAGGCAGAGTGGCTG
GTGGCCTGGAAATATCAACCTGTAGCTCAGGTTGCCGAAGTGAAGGCAATTCAGTTTGCG
GTGGGTAAGAGCGGTAAAATATCGGTGGTTGCGTCACTCGCACCTGTCATGCTGGATGAT
AAAAAAGTCCAGCGGGTGAATATTGGTTCCGTCAGGCGCTGGCAGGAGTGGGATATTGCG
CCTGGTGATCAGATTCTCGTCAGCCTTGCCGGTCAGGGTATTCCTCGCATTGATGATGTG
GTGTGGCGCGGTGCAGAACGTACAAAACCGACACCGCCAGAAAACCGCTTTAACTCGTTG
ACCTGCTACTTTGCTTCTGATGTTTGTCAGGAACAGTTCATTTCACGCTTAGTCTGGCTG
GGGGCAAAACAGGTTCTTGGGCTGGATGGCATTGGTGAGGCCGGTTGGCGCGCGCTGCAT
CAGACTCATCGCTTTGAACATATCTTTTCCTGGCTTTTATTAACGCCAGAGCAATTACAG
AACACGCCGGGGATCGCGAAAAGTAAAAGTGCGCAGCTATGGCATCAGTTTAATCTGGCT
CGTAAGCAGCCTTTTACTCGCTGGGTGATGGCAATGGGAATACCGCTAACCCGGGCGGCG
CTTAATGCCAGTGATGAACGGTCCTGGTCGCAACTTTTATTTAGCACGGAGCAGTTCTGG
CAGCAGCTGCCGGGGACTGGATCGGGACGCGCCAGACAGGTTATTGAATGGAAGGAAAAT
GCGCAAATCAAGAAGCTGGGCAGTTGGCTGGCTGCCCAGCAGATCACAGGTTTTGAACCT
TAG
Protein Properties
Pfam Domain Function:
Protein Residues:560
Protein Molecular Weight:63179
Protein Theoretical pI:9
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>DNA ligase B
MKVWMAILIGILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQWDDDYWKEGKSEVEDGV
YDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRKMVDKNALSLWMRERSDLW
VQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSLISAVPQTVSGPLANSTLQGE
IFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSLGVFVWAWPDGPQLMSDRLKELA
TAGFTLTQTYTRAVKNADEVARVRNEWWKAELPFVTDGVVVRAAKEPESRHWLPGQAEWL
VAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLAPVMLDDKKVQRVNIGSVRRWQEWDIA
PGDQILVSLAGQGIPRIDDVVWRGAERTKPTPPENRFNSLTCYFASDVCQEQFISRLVWL
GAKQVLGLDGIGEAGWRALHQTHRFEHIFSWLLLTPEQLQNTPGIAKSKSAQLWHQFNLA
RKQPFTRWVMAMGIPLTRAALNASDERSWSQLLFSTEQFWQQLPGTGSGRARQVIEWKEN
AQIKKLGSWLAAQQITGFEP
References
External Links:
ResourceLink
Uniprot ID:P25772
Uniprot Name:LIGB_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85676396
Ecogene ID:EG11334
Ecocyc:EG11334
ColiBase:b3647
Kegg Gene:b3647
EchoBASE ID:EB1310
CCDB:LIGB_ECOLI
BacMap:90111628
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Burland, V., Plunkett, G. 3rd, Daniels, D. L., Blattner, F. R. (1993). "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication." Genomics 16:551-561. Pubmed: 7686882
  • Gentry, D., Bengra, C., Ikehara, K., Cashel, M. (1993). "Guanylate kinase of Escherichia coli K-12." J Biol Chem 268:14316-14321. Pubmed: 8390989
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Sriskanda, V., Shuman, S. (2001). "A second NAD(+)-dependent DNA ligase (LigB) in Escherichia coli." Nucleic Acids Res 29:4930-4934. Pubmed: 11812821