2-octaprenyl-6-methoxyphenol hydroxylase (P25534)

Identification
Name:2-octaprenyl-6-methoxyphenol hydroxylase
Synonyms:Not Available
Gene Name:ubiH
Enzyme Class:Not Available
Biological Properties
General Function:Involved in oxidoreductase activity
Specific Function:Oxygenase that introduces the hydroxyl group at carbon four of 2-octaprenyl-6-methoxyphenol resulting in the formation of 2-octaprenyl-6-methoxy-1,4-benzoquinone
Cellular Location:Not Available
SMPDB Pathways:
  • Secondary Metabolites: Ubiquinol biosynthesis PW000981
  • Secondary Metabolites: Ubiquinol biosynthesis 2 PW002036
KEGG Pathways:
  • Ubiquinone and other terpenoid-quinone biosynthesis ec00130
KEGG Reactions:
1.02-Polyprenyl-6-methoxyphenol+1.0Thumb ↔ 1.02-Polyprenyl-6-methoxy-1,4-benzoquinone+1.0Thumb
1.02-Polyprenyl-6-methoxyphenol + 1.0Oxygen ↔ 1.02-Polyprenyl-6-methoxy-1,4-benzoquinone + 1.0Water
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0NADPH+1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0Hydrogen ion + 1.0NADPH + 1.0Oxygen + 1.02-methoxy-6-(all-trans-octaprenyl)phenol + 1.0NADPH → 1.0Water + 1.0NADP + 1.02-Octaprenyl-6-methoxy-1,4-benzoquinol
ReactionCard
EcoCyc Reactions:
1.0Thumb+1.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.02-Octaprenyl-6-methoxyphenol + 1.0Oxygen + 1.0NADPH + 1.0Hydrogen ion → 1.02-Octaprenyl-6-methoxy-1,4-benzoquinol + 1.0Water + 1.0NADP
ReactionCard
Complex Reactions:
1.0Thumb+0.5Thumb → 1.0Thumb
1.02-Octaprenyl-6-methoxyphenol + 0.5Oxygen → 1.02-Octaprenyl-6-methoxy-1,4-benzoquinol
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB234372-methoxy-6-(all-trans-octaprenyl)phenolMetaboCard
ECMDB211582-Octaprenyl-6-methoxy-1,4-benzoquinolMetaboCard
ECMDB041192-Octaprenyl-6-methoxyphenolMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB00217NADPMetaboCard
ECMDB04111NADPHMetaboCard
ECMDB04124OxygenMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
adenyl nucleotide binding
binding
catalytic activity
FAD or FADH2 binding
monooxygenase activity
nucleoside binding
oxidoreductase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NADH or NADPH as one donor, and incorporation of one atom of oxygen
purine nucleoside binding
Process
cellular metabolic process
coenzyme metabolic process
cofactor metabolic process
metabolic process
oxidation reduction
oxidoreduction coenzyme metabolic process
ubiquinone biosynthetic process
ubiquinone metabolic process
Gene Properties
Blattner:b2907
Gene OrientationCounterclockwise
Centisome Percentage:65.75
Left Sequence End3050362
Right Sequence End3051540
Gene Sequence:
>1179 bp
ATGATCTGGATAATGACGATGGCTCGCCGTATGAACGGTGTTTACGCGGCATTTATGCTG
GTCGCTTTTATGATGGGGGTGGCCGGGGCGCTACAGGCTCCTACATTGAGCTTATTTCTG
AGTCGTGAGGTTGGCGCGCAACCTTTCTGGATCGGCCTCTTTTATACGGTGAATGCTATT
GCTGGGATCGGCGTAAGCCTCTGGTTGGCAAAACGTTCTGACAGTCAGGGCGATCGGCGA
AAACTGATTATATTTTGCTGTTTGATGGCTATCGGCAATGCGCTATTGTTTGCATTTAAT
CGTCATTATCTGACGCTTATCACCTGTGGTGTGCTTCTGGCATCTCTGGCCAATACGGCA
ATGCCACAGTTATTTGCTCTGGCGCGGGAATATGCGGATAACTCGGCGCGAGAAGTGGTG
ATGTTTAGCTCGGTGATGCGTGCGCAGCTTTCTCTGGCATGGGTTATCGGTCCACCGTTG
GCCTTTATGCTGGCGTTGAATTACGGCTTTACGGTGATGTTTTCGATTGCCGCCGGGATA
TTCACACTCAGTCTGGTATTGATTGCATTTATGCTTCCGTCTGTGGCGCGGGTAGAACTG
CCGTCGGAAAATGCTTTATCAATGCAAGGTGGCTGGCAGGATAGTAACGTACGGATGTTA
TTTGTCGCCTCGACGTTAATGTGGACCTGCAACACCATGTACATTATTGATATGCCGTTG
TGGATCAGTAGCGAGTTAGGATTGCCAGACAAACTGGCGGGTTTCCTGATGGGGACGGCA
GCTGGACTGGAAATACCAGCAATGATTCTGGCTGGCTACTATGTCAAACGTTATGGTAAG
CGGCGAATGATGGTCATAGCAGTGGCGGCAGGAGTACTGTTTTACACCGGATTGATTTTC
TTTAATAGCCGTATGGCGTTGATGACGCTGCAACTTTTTAACGCTGTATTTATCGGCATT
GTTGCGGGTATTGGGATGCTATGGTTTCAGGATTTAATGCCTGGAAGAGCGGGGGCAGCT
ACCACCTTATTTACTAACAGTATTTCTACCGGGGTAATTCTGGCTGGCGTTATTCAGGGA
GCAATTGCACAAAGTTGGGGGCACTTTGCTGTCTACTGGGTAATTGCGGTTATTTCTGTT
GTCGCATTATTTTTAACCGCAAAGGTTAAAGACGTTTGA
Protein Properties
Pfam Domain Function:
Protein Residues:392
Protein Molecular Weight:42288
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>2-octaprenyl-6-methoxyphenol hydroxylase
MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPGFDGRAIALAAGTCQQL
ARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAALGQVVELHNVGQRLFALLR
KAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVAADGTHSALATACGVDWQQEP
YEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDGRCSLVWCHPLERREEVLSWSDE
KFCRELQSAFGWRLGKITHAGKRSAYPLALTHAARSITHRTVLVGNAAQTLHPIAGQGFN
LGMRDVMSLAETLTQAQERGEDMGDYGVLCRYQQRRQSDREATIGVTDSLVHLFANRWAP
LVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
References
External Links:
ResourceLink
Uniprot ID:P25534
Uniprot Name:UBIH_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674315
Ecogene ID:EG11324
Ecocyc:EG11324
ColiBase:b2907
Kegg Gene:b2907
EchoBASE ID:EB1300
CCDB:UBIH_ECOLI
BacMap:16130809
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Nakahigashi, K., Miyamoto, K., Nishimura, K., Inokuchi, H. (1992). "Isolation and characterization of a light-sensitive mutant of Escherichia coli K-12 with a mutation in a gene that is required for the biosynthesis of ubiquinone." J Bacteriol 174:7352-7359. Pubmed: 1339425