| Identification |
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| Name: | ATP-dependent DNA helicase recG |
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| Synonyms: | Not Available |
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| Gene Name: | recG |
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| Enzyme Class: | |
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| Biological Properties |
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| General Function: | Replication, recombination and repair |
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| Specific Function: | Critical role in recombination and DNA repair. Help process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3' to 5' polarity. recG unwind branched duplex DNA (Y-DNA). Has a role in constitutive stable DNA replication (csdR) and R-loop formation |
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| Cellular Location: | Cytoplasmic |
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| SMPDB Pathways: | Not Available |
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| KEGG Pathways: | |
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| Metabolites: | |
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| GO Classification: | | Function |
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| adenyl nucleotide binding | | adenyl ribonucleotide binding | | ATP binding | | ATP-dependent DNA helicase activity | | ATP-dependent helicase activity | | ATPase activity | | ATPase activity, coupled | | binding | | catalytic activity | | DNA helicase activity | | helicase activity | | hydrolase activity | | hydrolase activity, acting on acid anhydrides | | hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides | | nucleic acid binding | | nucleoside binding | | nucleoside-triphosphatase activity | | purine nucleoside binding | | pyrophosphatase activity | | Process |
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| cellular macromolecule metabolic process | | DNA metabolic process | | DNA recombination | | DNA repair | | macromolecule metabolic process | | metabolic process |
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| Gene Properties |
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| Blattner: | Not Available |
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| Gene Orientation | Not Available |
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| Centisome Percentage: | Not Available |
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| Left Sequence End | Not Available |
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| Right Sequence End | Not Available |
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| Gene Sequence: | >2082 bp
ATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCGCAGCA
CTTAGTAACAAACTGGCGAAAATCAACCTGCATACCGTGCAGGATCTACTCTTACACCTT
CCCCTGCGCTACGAAGATCGCACCCATCTCTACCCCATCGGAGAACTACTGCCGGGCGTT
TATGCCACGGTGGAAGGCGAAGTGCTGAACTGCAATATCTCCTTCGGCGGTCGGCGGATG
ATGACCTGCCAGATCAGCGACGGTTCCGGCATCCTCACCATGCGCTTTTTCAATTTCAGC
GCGGCAATGAAAAATAGCCTGGCGGCGGGCCGCCGTGTACTGGCTTATGGCGAAGCAAAG
CGCGGTAAATATGGTGCGGAGATGATCCACCCGGAATACCGCGTGCAGGGCGATCTCAGC
ACGCCAGAATTACAGGAAACGCTCACGCCGGTTTATCCAACAACGGAAGGCGTAAAGCAG
GCCACGCTGCGTAAATTAACCGACCAGGCGCTGGATCTGCTCGACACCTGCGCCATTGAA
GAACTCCTGCCGCCGGAACTGTCACAAGGAATGATGACGCTACCGGAAGCGTTGCGCACT
TTGCACCGCCCGCCACCGACGCTACAGCTTAGCGATCTGGAAACCGGGCAGCATCCGGCG
CAACGTCGTCTGATTCTGGAAGAACTGCTGGCGCACAACCTCAGCATGTTAGCCTTACGT
GCCGGAGCACAGCGTTTTCATGCCCAGCCGCTGAGCGCCAATGACACGCTGAAAAATAAA
CTCCTCGCCGCCTTACCGTTCAAGCCAACGGGCGCACAGGCACGCGTAGTGGCGGAGATC
GAGCGCGATATGGCGCTGGATGTGCCGATGATGCGTCTGGTGCAGGGCGATGTAGGTTCC
GGTAAAACGCTGGTCGCCGCCCTCGCCGCGTTGCGTGCGATTGCCCACGGCAAACAGGTA
GCATTGATGGCACCAACCGAATTACTCGCCGAGCAGCACGCCAATAACTTCCGCAACTGG
TTTGCACCGCTCGGTATCGAAGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCACGG
CTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGATGATTGTCGGTACACACGCC
ATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCAT
CGTTTTGGCGTGCATCAGCGTCTGGCATTGTGGGAGAAAGGCCAGCAGCAGGGCTTCCAT
CCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTAT
GCCGATCTCGATACCTCGGTGATAGATGASSTGCCGCCAGGCCGCACGCCAGTGACTACG
GTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATA
ACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAA
GCGCAGGCGGCGGAAGCTACCTGGGAAGAGTTGAAACTGGCGCTACCAGAGTTGAACGTT
GGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGGCGGTGATGGCGTCGTTTAAA
CAAGGTGAGCTACACCTGCTGGTTGCCACAACCGTTATTGAAGTCGGCGTTGATGTGCCT
AACGCCAGTCTGATGATTATCGAAAACCCGGAGCGTCTGGGTCTGGCGCAGTTACACCAG
CTGCGCGGGCGCGTAGGTCGTGGCGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACG
CCGCTTTCTAAAACGGCGCAAATTCGCCTGCAAGTSSTGCGCGACAGTAACGACGGTTTT
GTGATTGCGCAAAAAGATCTGGAGATTCGCGGCCCTGGCGAATTGTTAGGCACGCGTCAG
ACGGGTAATGCTGAATTTAAAGTGGCGGATTTACTGCGCGATCAGGCGATGATCCCGGAA
GTTCAGCGCCTGGCACGCCATATTCACGAACGTTACCCACAACAGGCAAAAGCCCTGATA
GAACGCTGGATGCCGGAGACGGAACGTTACTCGAATGCGTAA |
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| Protein Properties |
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| Pfam Domain Function: | |
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| Protein Residues: | 693 |
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| Protein Molecular Weight: | 76430 |
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| Protein Theoretical pI: | 7 |
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| Signaling Regions: | |
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| Transmembrane Regions: | |
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| Protein Sequence: | >ATP-dependent DNA helicase recG
MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRYEDRTHLYPIGELLPGV
YATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAAMKNSLAAGRRVLAYGEAK
RGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQATLRKLTDQALDLLDTCAIE
ELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQHPAQRRLILEELLAHNLSMLALR
AGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARVVAEIERDMALDVPMMRLVQGDVGS
GKTLVAALAALRAIAHGKQVALMAPTELLAEQHANNFRNWFAPLGIEVGWLAGKQKGKAR
LAQQEAIASGQVQMIVGTHAIFQEQVQFNGLALVIIDEQHRFGVHQRLALWEKGQQQGFH
PHQLIMTATPIPRTLAMTAYADLDTSVIDELPPGRTPVTTVAIPDTRRTDIIDRVHHACI
TEGRQAYWVCTLIEESELLEAQAAEATWEELKLALPELNVGLVHGRMKPAEKQAVMASFK
QGELHLLVATTVIEVGVDVPNASLMIIENPERLGLAQLHQLRGRVGRGAVASHCVLLYKT
PLSKTAQIRLQVLRDSNDGFVIAQKDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPE
VQRLARHIHERYPQQAKALIERWMPETERYSNA |
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| References |
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| External Links: | |
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| General Reference: | - Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
- Burland, V., Plunkett, G. 3rd, Daniels, D. L., Blattner, F. R. (1993). "DNA sequence and analysis of 136 kilobases of the Escherichia coli genome: organizational symmetry around the origin of replication." Genomics 16:551-561. Pubmed: 7686882
- Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
- Hong, X., Cadwell, G. W., Kogoma, T. (1995). "Escherichia coli RecG and RecA proteins in R-loop formation." EMBO J 14:2385-2392. Pubmed: 7774596
- Kalman, M., Murphy, H., Cashel, M. (1992). "The nucleotide sequence of recG, the distal spo operon gene in Escherichia coli K-12." Gene 110:95-99. Pubmed: 1544582
- Lloyd, R. G., Sharples, G. J. (1991). "Molecular organization and nucleotide sequence of the recG locus of Escherichia coli K-12." J Bacteriol 173:6837-6843. Pubmed: 1938888
- Lombardo, M. J., Rosenberg, S. M. (2000). "radC102 of Escherichia coli is an allele of recG." J Bacteriol 182:6287-6291. Pubmed: 11053371
- Whitby, M. C., Vincent, S. D., Lloyd, R. G. (1994). "Branch migration of Holliday junctions: identification of RecG protein as a junction specific DNA helicase." EMBO J 13:5220-5228. Pubmed: 7957087
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