Mannonate dehydratase (P24215)

Identification
Name:Mannonate dehydratase
Synonyms:
  • D-mannonate hydrolase
Gene Name:uxuA
Enzyme Class:
Biological Properties
General Function:Involved in mannonate dehydratase activity
Specific Function:D-mannonate = 2-dehydro-3-deoxy-D-gluconate + H(2)O
Cellular Location:Not Available
SMPDB Pathways:
  • hexuronide and hexuronate degradation PW000834
KEGG Pathways:
KEGG Reactions:
1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0D-Mannonate ↔ 1.02-Keto-3-deoxy-D-gluconic acid + 1.0Water
ReactionCard
SMPDB Reactions:
1.0Thumb → 1.0Thumb+1.0Thumb+1.0Thumb
1.0 D-altronate → 1.0Water + 1.02-Dehydro-3-deoxy-D-galactonate + 1.02-Keto-3-deoxy-D-gluconic acid
ReactionCard
EcoCyc Reactions:
1.0Thumb ↔ 1.0Thumb+1.0Thumb
1.0D-Mannonate ↔ 1.02-Keto-3-deoxy-D-gluconic acid + 1.0Water
ReactionCard
1.0Thumb → 1.0Thumb+1.0Thumb
1.0D-Mannonate → 1.0Water + 1.02-Keto-3-deoxy-D-gluconic acid
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB24229 D-altronateMetaboCard
ECMDB013532-Keto-3-deoxy-D-gluconic acidMetaboCard
ECMDB04101D-MannonateMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Function
carbon-oxygen lyase activity
catalytic activity
hydro-lyase activity
lyase activity
mannonate dehydratase activity
Process
carbohydrate catabolic process
carbohydrate metabolic process
glucuronate catabolic process
metabolic process
primary metabolic process
Gene Properties
Blattner:b4322
Gene OrientationClockwise
Centisome Percentage:98.06
Left Sequence End4549659
Right Sequence End4550843
Gene Sequence:
>1185 bp
ATGAAAAATTGTGTCATCGTCAGTGCGGTACGTACTGCTATCGGTAGTTTTAACGGTTCA
CTCGCTTCCACCAGCGCCATCGACCTGGGGGCGACAGTAATTAAAGCCGCCATTGAACGT
GCAAAAATCGATTCACAACACGTTGATGAAGTGATTATGGGTAACGTGTTACAAGCCGGG
CTGGGGCAAAATCCGGCGCGTCAGGCACTGTTAAAAAGCGGGCTGGCAGAAACGGTGTGC
GGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAG
GCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTA
GCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTT
TATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCATATGGGGATT
ACCGCCGAAAACGTGGCTAAAGAGTACGGAATTACCCGTGAAATGCAGGATGAACTGGCG
CTACATTCACAGCGTAAAGCGGCAGCCGCAATTGAGTCCGGTGCTTTTACAGCCGAAATC
GTCCCGGTAAATGTTGTCACTCGAAAGAAAACCTTCGTCTTCAGTCAAGACGAATTCCCG
AAAGCGAATTCAACGGCTGAAGCGTTAGGTGCATTGCGCCCGGCCTTCGATAAAGCAGGA
ACAGTCACCGCTGGGAACGCGTCTGGTATTAACGACGGTGCTGCCGCTCTGGTGATTATG
GAAGAATCTGCGGCGCTGGCAGCAGGCCTTACCCCCCTGGCTCGCATTAAAAGTTATGCC
AGCGGTGGCGTGCCCCCCGCATTGATGGGTATGGGGCCAGTACCTGCCACGCAAAAAGCG
TTACAACTGGCGGGGCTGCAACTGGCGGATATTGATCTCATTGAGGCTAATGAAGCATTT
GCTGCACAGTTCCTTGCCGTTGGGAAAAACCTGGGCTTTGATTCTGAGAAAGTGAATGTC
AACGGCGGGGCCATCGCGCTCGGGCATCCTATCGGTGCCAGTGGTGCTCGTATTCTGGTC
ACACTATTACATGCCATGCAGGCACGCGATAAAACGCTGGGGCTGGCAACACTGTGCATT
GGCGGCGGTCAGGGAATTGCGATGGTGATTGAACGGTTGAATTAA
Protein Properties
Pfam Domain Function:
Protein Residues:394
Protein Molecular Weight:44838
Protein Theoretical pI:5
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Mannonate dehydratase
MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEEILKRKAIIEDAGLVWS
VVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCYNFMPVLDWTRTDLEYVLP
DGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAERFATMSDEDKARLTRNIIAG
LPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKAIIPVAEEVGVRMAVHPDDPPRP
ILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYGVRADNDLVDMIKQFGPRIYFTHLR
STMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEEHRRKAEGKEDLIPMRPDHGHQMLDDL
KKKTNPGYSAIGRLKGLAEVRGVELAIQRAFFSR
References
External Links:
ResourceLink
Uniprot ID:P24215
Uniprot Name:UXUA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:1736879
Ecogene ID:EG11066
Ecocyc:EG11066
ColiBase:b4322
Kegg Gene:b4322
EchoBASE ID:EB1059
CCDB:UXUA_ECOLI
BacMap:16132143
General Reference:
  • Blanco, C., Ritzenthaler, P., Kolb, A. (1986). "The regulatory region of the uxuAB operon in Escherichia coli K12." Mol Gen Genet 202:112-119. Pubmed: 3083215
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Burland, V., Plunkett, G. 3rd, Sofia, H. J., Daniels, D. L., Blattner, F. R. (1995). "Analysis of the Escherichia coli genome VI: DNA sequence of the region from 92.8 through 100 minutes." Nucleic Acids Res 23:2105-2119. Pubmed: 7610040
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Klemm, P., Tong, S., Nielsen, H., Conway, T. (1996). "The gntP gene of Escherichia coli involved in gluconate uptake." J Bacteriol 178:61-67. Pubmed: 8550444
  • Marc, D., Arne, P., Bree, A., Dho-Moulin, M. (1998). "Colonization ability and pathogenic properties of a fim- mutant of an avian strain of Escherichia coli." Res Microbiol 149:473-485. Pubmed: 9766199