Glutamate-1-semialdehyde 2,1-aminomutase (P23893)

Identification
Name:Glutamate-1-semialdehyde 2,1-aminomutase
Synonyms:
  • GSA
  • Glutamate-1-semialdehyde aminotransferase
  • GSA-AT
Gene Name:hemL
Enzyme Class:
Biological Properties
General Function:Involved in glutamate-1-semialdehyde 2,1-aminomutase activity
Specific Function:(S)-4-amino-5-oxopentanoate = 5- aminolevulinate
Cellular Location:Cytoplasm (Potential)
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb ↔ 1.0Thumb
1.0(S)-4-Amino-5-oxopentanoate ↔ 1.05-Aminolevulinic acid
ReactionCard
1.0Thumb ↔ 1.0Thumb
1.05-Aminolevulinic acid ↔ 1.0(S)-4-Amino-5-oxopentanoate
ReactionCard
SMPDB Reactions:
8.0Thumb → 8.0Thumb
8.0(S)-4-Amino-5-oxopentanoate → 8.05-Aminolevulinic acid
ReactionCard
EcoCyc Reactions:
1.0Thumb ↔ 1.0Thumb
1.0(S)-4-Amino-5-oxopentanoate ↔ 1.05-Aminolevulinic acid
ReactionCard
1.0Thumb ↔ 1.0Thumb
1.05-Aminolevulinic acid ↔ 1.0(S)-4-Amino-5-oxopentanoate
ReactionCard
Complex Reactions:
1.0Thumb → 1.0Thumb
1.0(S)-4-Amino-5-oxopentanoate → 1.05-Aminolevulinic acid
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB20017(S)-4-Amino-5-oxopentanoateMetaboCard
ECMDB011495-Aminolevulinic acidMetaboCard
GO Classification:
Function
binding
catalytic activity
cofactor binding
glutamate-1-semialdehyde 2,1-aminomutase activity
intramolecular transferase activity
intramolecular transferase activity, transferring amino groups
isomerase activity
pyridoxal phosphate binding
transaminase activity
transferase activity
transferase activity, transferring nitrogenous groups
Process
biosynthetic process
cellular biosynthetic process
heterocycle biosynthetic process
metabolic process
tetrapyrrole biosynthetic process
Gene Properties
Blattner:b0154
Gene OrientationCounterclockwise
Centisome Percentage:3.74
Left Sequence End173602
Right Sequence End174882
Gene Sequence:
>1281 bp
ATGAGTAAGTCTGAAAATCTTTACAGCGCAGCGCGCGAGCTGATCCCTGGCGGTGTGAAC
TCCCCTGTTCGCGCCTTTACTGGCGTGGGCGGCACTCCACTGTTTATCGAAAAAGCGGAC
GGCGCTTATCTGTACGATGTTGATGGCAAAGCCTATATCGATTATGTCGGTTCCTGGGGG
CCGATGGTGCTGGGCCATAACCATCCGGCAATCCGCAATGCCGTGATTGAAGCCGCCGAG
CGTGGTTTAAGCTTTGGTGCACCAACCGAAATGGAAGTGAAAATGGCGCAACTGGTGACC
GAACTGGTCCCGACCATGGATATGGTGCGCATGGTGAACTCCGGCACTGAAGCGACCATG
AGCGCCATCCGCCTGGCCCGTGGTTTTACCGGTCGCGACAAAATTATTAAATTTGAAGGG
TGTTACCATGGTCACGCTGACTGCCTGCTGGTGAAAGCCGGTTCTGGCGCACTCACGTTA
GGCCAGCCAAACTCGCCGGGCGTTCCGGCAGATTTCGCCAAATATACCTTAACCTGTACT
TATAATGATCTGGCTTCTGTACGCGCCGCATTTGAGCAATACCCGCAAGAGATTGCCTGT
ATTATCGTCGAGCCGGTGGCAGGCAATATGAACTGTGTTCCGCCGCTGCCAGAGTTCCTG
CCAGGTCTGCGCGCGCTGTGCGACGAATTTGGCGCGTTGCTGATCATCGATGAAGTGATG
ACCGGTTTCCGCGTAGCGCTAGCTGGCGCACAGGATTATTACGGCGTAGTGCCAGATTTA
ACCTGCCTCGGCAAAATCATCGGCGGTGGAATGCCGGTAGGCGCATTCGGTGGTCGTCGT
GATGTAATGGATGCGCTGGCCCCGACGGGTCCGGTCTATCAGGCGGGTACGCTTTCCGGT
AACCCGATTGCGATGGCAGCGGGTTTCGCCTGTCTGAATGAAGTCGCGCAGCCGGGCGTT
CACGAAACGCTGGATGAGCTGACAACACGTCTGGCAGAAGGTCTGCTGGAAGCGGCAGAA
GAAGCCGGAATTCCGCTGGTCGTTAACCACGTTGGCGGCATGTTCGGTATTTTCTTTACC
GACGCCGAGTCCGTGACGTGCTATCAGGATGTGATGGCCTGTGACGTGGAACGCTTTAAG
CGTTTCTTCCATATGATGCTGGACGAAGGTGTTTACCTGGCACCGTCAGCGTTTGAAGCG
GGCTTTATGTCCGTGGCGCACAGCATGGAAGATATCAATAACACCATCGATGCTGCACGT
CGGGTGTTTGCGAAGTTGTGA
Protein Properties
Pfam Domain Function:
Protein Residues:426
Protein Molecular Weight:45366
Protein Theoretical pI:4
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Glutamate-1-semialdehyde 2,1-aminomutase
MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYLYDVDGKAYIDYVGSWG
PMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTELVPTMDMVRMVNSGTEATM
SAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTLGQPNSPGVPADFAKYTLTCT
YNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPEFLPGLRALCDEFGALLIIDEVM
TGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAFGGRRDVMDALAPTGPVYQAGTLSG
NPIAMAAGFACLNEVAQPGVHETLDELTTRLAEGLLEAAEEAGIPLVVNHVGGMFGIFFT
DAESVTCYQDVMACDVERFKRFFHMMLDEGVYLAPSAFEAGFMSVAHSMEDINNTIDAAR
RVFAKL
References
External Links:
ResourceLink
Uniprot ID:P23893
Uniprot Name:GSA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674360
Ecogene ID:EG10432
Ecocyc:EG10432
ColiBase:b0154
Kegg Gene:b0154
EchoBASE ID:EB0427
CCDB:GSA_ECOLI
BacMap:16128147
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Fujita, N., Mori, H., Yura, T., Ishihama, A. (1994). "Systematic sequencing of the Escherichia coli genome: analysis of the 2.4-4.1 min (110,917-193,643 bp) region." Nucleic Acids Res 22:1637-1639. Pubmed: 8202364
  • Grimm, B., Bull, A., Breu, V. (1991). "Structural genes of glutamate 1-semialdehyde aminotransferase for porphyrin synthesis in a cyanobacterium and Escherichia coli." Mol Gen Genet 225:1-10. Pubmed: 1900346
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Ilag, L. L., Jahn, D. (1992). "Activity and spectroscopic properties of the Escherichia coli glutamate 1-semialdehyde aminotransferase and the putative active site mutant K265R." Biochemistry 31:7143-7151. Pubmed: 1643048
  • Ilag, L. L., Jahn, D., Eggertsson, G., Soll, D. (1991). "The Escherichia coli hemL gene encodes glutamate 1-semialdehyde aminotransferase." J Bacteriol 173:3408-3413. Pubmed: 2045363
  • Minagawa, S., Ogasawara, H., Kato, A., Yamamoto, K., Eguchi, Y., Oshima, T., Mori, H., Ishihama, A., Utsumi, R. (2003). "Identification and molecular characterization of the Mg2+ stimulon of Escherichia coli." J Bacteriol 185:3696-3702. Pubmed: 12813061