UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase (P21645)

Identification
Name:UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase
Synonyms:
  • Protein firA
  • Rifampicin resistance protein
Gene Name:lpxD
Enzyme Class:
Biological Properties
General Function:Involved in transferase activity
Specific Function:UDP-3-O-(3-hydroxytetradecanoyl)glucosamine + (R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] = UDP-2,3-bis(3- hydroxytetradecanoyl)glucosamine + [acyl-carrier-protein]
Cellular Location:Not Available
SMPDB Pathways:
KEGG Pathways:
KEGG Reactions:
1.0Thumb+1.0Acyl-carrier protein+1.0(3R)-3-Hydroxytetradecanoyl-[acyl-carrier protein] ↔ 1.0Thumb+1.0(3R)-3-Hydroxytetradecanoyl-[acyl-carrier protein]+1.0Acyl-carrier protein
1.0UDP-2,3-Bis(3-hydroxytetradecanoyl)glucosamine + 1.0Acyl-carrier protein + 1.0(3R)-3-Hydroxytetradecanoyl-[acyl-carrier protein] ↔ 1.0UDP-3-O-(3-Hydroxytetradecanoyl)-D-glucosamine + 1.0(3R)-3-Hydroxytetradecanoyl-[acyl-carrier protein] + 1.0Acyl-carrier protein
ReactionCard
SMPDB Reactions:
1.0Thumb+1.0(3R)-3-hydroxymyristoyl-[acp] → 1.0Thumb+1.0a holo-[acyl-carrier protein]+1.0Thumb
1.0UDP-3-O-(3-Hydroxytetradecanoyl)-D-glucosamine + 1.0(3R)-3-hydroxymyristoyl-[acp] → 1.0Hydrogen ion + 1.0a holo-[acyl-carrier protein] + 1.0UDP-2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-α-D-glucosamine
ReactionCard
Complex Reactions:
1.0(R)-3-Hydroxytetradecanoyl-[acyl-carrier protein]+1.0Thumb → 1.0acyl carrier protein+1.0Thumb+1.0Thumb
1.0(R)-3-Hydroxytetradecanoyl-[acyl-carrier protein] + 1.0UDP-3-O-(3-Hydroxytetradecanoyl)-D-glucosamine → 1.0acyl carrier protein + 1.0Hydrogen ion + 1.0UDP-2,3-Bis(3-hydroxytetradecanoyl)glucosamine
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB214933-Hydroxymyristic acidMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB20201UDP-2,3-Bis(3-hydroxytetradecanoyl)glucosamineMetaboCard
ECMDB23134UDP-2,3-bis(O-(3R)-3-Hydroxymyristoyl)-alpha-D-glucosamineMetaboCard
ECMDB24216UDP-2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-α-D-glucosamineMetaboCard
ECMDB21292UDP-3-O-(3-Hydroxytetradecanoyl)-D-glucosamineMetaboCard
GO Classification:
Function
catalytic activity
transferase activity
transferase activity, transferring acyl groups
transferase activity, transferring acyl groups other than amino-acyl groups
Process
lipid A biosynthetic process
metabolic process
organophosphate metabolic process
phospholipid biosynthetic process
phospholipid metabolic process
Gene Properties
Blattner:b0179
Gene OrientationClockwise
Centisome Percentage:4.33
Left Sequence End200971
Right Sequence End201996
Gene Sequence:
>1026 bp
ATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGAT
GGCGATATCGTCATCACCGGCGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACG
TTCATGGTTAACCCAAAATACCGTGAGCATTTAGGCTTGTGCCAGGCGTCCGCGGTTGTC
ATGACCCAGGACGATCTTCCTTTCGCGAAAAGTGCCGCACTGGTAGTGAAGAATCCCTAC
CTGACTTACGCGCGCATGGCGCAAATTTTAGATACCACGCCGCAGCCCGCGCAGAACATT
GCACCCAGTGCGGTGATCGACGCGACGGCGAAGCTGGGTAACAACGTATCGATTGGCGCT
AACGCGGTGATTGAGTCCGGCGTTGAACTGGGCGATAACGTGATTATCGGTGCCGGTTGC
TTCGTAGGTAAAAACAGCAAAATCGGTGCAGGTTCGCGTCTCTGGGCGAACGTAACCATT
TACCATGAGATCCAGATCGGTCAGAATTGCCTGATCCAGTCCGGAACAGTGGTAGGCGCA
GACGGCTTTGGTTATGCCAACGATCGTGGTAACTGGGTGAAGATCCCACAGATTGGTCGC
GTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACAACCATCGATCGCGGCGCGCTG
GATGACACTATTATTGGCAATGGCGTGATCATTGATAACCAGTGCCAGATTGCACATAAC
GTCGTGATTGGCGACAATACGGCGGTTGCCGGTGGCGTCATTATGGCGGGCAGCCTGAAA
ATTGGTCGTTACTGCATGATCGGCGGAGCCAGCGTAATCAACGGGCATATGGAAATATGC
GACAAAGTGACGGTTACGGGCATGGGTATGGTGATGCGTCCCATCACTGAACCAGGCGTC
TATTCCTCAGGCATTCCGCTGCAACCCAACAAAGTCTGGCGCAAAACCGCTGCACTGGTG
ATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAA
GACTAA
Protein Properties
Pfam Domain Function:
Protein Residues:341
Protein Molecular Weight:36038
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase
MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVNPKYREHLGLCQASAVV
MTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAPSAVIDATAKLGNNVSIGA
NAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTIYHEIQIGQNCLIQSGTVVGA
DGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRGALDDTIIGNGVIIDNQCQIAHN
VVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGHMEICDKVTVTGMGMVMRPITEPGV
YSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLERKVNQQD
References
External Links:
ResourceLink
Uniprot ID:P21645
Uniprot Name:LPXD_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4902920
Ecogene ID:EG10316
Ecocyc:EG10316
ColiBase:b0179
Kegg Gene:b0179
EchoBASE ID:EB0312
CCDB:LPXD_ECOLI
BacMap:16128172
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Dicker, I. B., Seetharam, S. (1991). "Cloning and nucleotide sequence of the firA gene and the firA200(Ts) allele from Escherichia coli." J Bacteriol 173:334-344. Pubmed: 1987124
  • Dicker, I. B., Seetharam, S. (1992). "What is known about the structure and function of the Escherichia coli protein FirA?" Mol Microbiol 6:817-823. Pubmed: 1602961
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Helander, I. M., Lindner, B., Seydel, U., Vaara, M. (1993). "Defective biosynthesis of the lipid A component of temperature-sensitive firA (omsA) mutant of Escherichia coli." Eur J Biochem 212:363-369. Pubmed: 8444173
  • Kelly, T. M., Stachula, S. A., Raetz, C. R., Anderson, M. S. (1993). "The firA gene of Escherichia coli encodes UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase. The third step of endotoxin biosynthesis." J Biol Chem 268:19866-19874. Pubmed: 8366125
  • Vuorio, R., Vaara, M. (1992). "Mutants carrying conditionally lethal mutations in outer membrane genes omsA and firA (ssc) are phenotypically similar, and omsA is allelic to firA." J Bacteriol 174:7090-7097. Pubmed: 1429432