Cyclic di-GMP phosphodiesterase yahA (P21514)

Identification
Name:Cyclic di-GMP phosphodiesterase yahA
Synonyms:Not Available
Gene Name:yahA
Enzyme Class:
Biological Properties
General Function:Involved in two-component response regulator activity
Specific Function:Hydrolyzes c-di-GMP (cyclic bis(3'-5') dimeric GMP) to 5'-pGpG, known as PDE-A activity. PDE-B activity, that is hydrolysis of 5'-pGpG to GMP, proceeds only very slowly
Cellular Location:Cytoplasmic
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
KEGG Reactions:
1.03',5'-Cyclic diGMP+1.0Thumb+1.0Thumb ↔ 1.0Thumb
1.03',5'-Cyclic diGMP + 1.0Water + 1.0Cyclic di-3',5'-guanylate ↔ 1.0Linear dimeric GMP
ReactionCard
EcoCyc Reactions:
1.0cyclic di-3',5'-guanylate+1.0Thumb → 1.0Thumb+1.0Thumb
1.0cyclic di-3',5'-guanylate + 1.0Water → 1.0Hydrogen ion + 1.0Linear dimeric GMP
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB23177Cyclic di-3',5'-guanylateMetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB20579Linear dimeric GMPMetaboCard
ECMDB00494WaterMetaboCard
GO Classification:
Component
cell part
intracellular
Function
binding
DNA binding
molecular transducer activity
nucleic acid binding
sequence-specific DNA binding
sequence-specific DNA binding transcription factor activity
signal transducer activity
two-component response regulator activity
Process
biological regulation
regulation of biological process
regulation of gene expression
regulation of macromolecule metabolic process
regulation of metabolic process
regulation of transcription
regulation of transcription, DNA-dependent
signal transmission
signaling process
two-component signal transduction system (phosphorelay)
Gene Properties
Blattner:b0315
Gene OrientationClockwise
Centisome Percentage:7.15
Left Sequence End331595
Right Sequence End332683
Gene Sequence:
>1089 bp
ATGAATTCATGTGATTTTCGTGTTTTTCTGCAAGAGTTCGGTACAACGGTTCATTTGTCA
TTGCCTGGTAGCGTATCCGAGAAAGAACGACTGCTACTCAAGCTGCTGATGCAGGGAATG
TCTGTAACAGAAATATCACAGTACAGAAATCGCAGTGCAAAGACAATTTCACATCAAAAG
AAACAGCTCTTTGAGAAACTGGGGATTCAGAGCGATATTACTTTCTGGCGCGATATTTTC
TTTCAGTACAATCCGGAGATCATATCCGCCACGGGGAGTAATAGTCACAGATATATTAAT
GATAATCACTATCACCATATCGTCACGCCTGAAGCCATCAGTCTGGCGTTGGAAAACCAC
GAATTCAAACCGTGGATCCAACCGGTTTTCTGCGCGCAGACTGGCGTACTGACGGGCTGT
GAGGTGCTTGTCCGCTGGGAACATCCACAAACGGGAATTATCCCACCGGATCAGTTTATT
CCTCTGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACT
GCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAAC
GTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTTAAT
AAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAACGTAACCCTATTCCG
GTAACGCCAGAAGCCAGAGCGATATTTGACAGCCTTCATCAGCACAACATTACCTTTGCG
CTGGATGACTTTGGTACGGGTTATGCGACCTATCGTTACTTGCAGGCGTTCCCGGTCGAT
TTTATTAAGATCGATAAGTCATTTGTGCAAATGGCGAGTGTCGACGAAATCTCCGGTCAT
ATTGTGGACAATATTGTCGAACTAGCGCGTAAGCCTGGTCTGAGTATCGTGGCGGAAGGG
GTAGAAACCCAGGAGCAGGCGGATTTAATGATCGGTAAAGGCGTTCACTTTTTGCAGGGC
TATTTGTACTCTCCGCCAGTACCGGGTAATAAATTTATCTCTGAATGGGTAATGAAAGCA
GGTGGTTGA
Protein Properties
Pfam Domain Function:
Protein Residues:362
Protein Molecular Weight:40725
Protein Theoretical pI:6
Signaling Regions:
  • None
Transmembrane Regions:
  • None
Protein Sequence:
>Cyclic di-GMP phosphodiesterase yahA
MNSCDFRVFLQEFGTTVHLSLPGSVSEKERLLLKLLMQGMSVTEISQYRNRSAKTISHQK
KQLFEKLGIQSDITFWRDIFFQYNPEIISATGSNSHRYINDNHYHHIVTPEAISLALENH
EFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFIPLAESSGLIVIMTRQLMKQT
ADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNLVNKLGNDKIKLVLELTERNPIP
VTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQAFPVDFIKIDKSFVQMASVDEISGH
IVDNIVELARKPGLSIVAEGVETQEQADLMIGKGVHFLQGYLYSPPVPGNKFISEWVMKA
GG
References
External Links:
ResourceLink
Uniprot ID:P21514
Uniprot Name:YAHA_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:85674458
Ecogene ID:EG11236
Ecocyc:EG11236
ColiBase:b0315
Kegg Gene:b0315
EchoBASE ID:EB1218
CCDB:YAHA_ECOLI
BacMap:16128300
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553
  • Lamark, T., Kaasen, I., Eshoo, M. W., Falkenberg, P., McDougall, J., Strom, A. R. (1991). "DNA sequence and analysis of the bet genes encoding the osmoregulatory choline-glycine betaine pathway of Escherichia coli." Mol Microbiol 5:1049-1064. Pubmed: 1956285
  • Schmidt, A. J., Ryjenkov, D. A., Gomelsky, M. (2005). "The ubiquitous protein domain EAL is a cyclic diguanylate-specific phosphodiesterase: enzymatically active and inactive EAL domains." J Bacteriol 187:4774-4781. Pubmed: 15995192