Formate hydrogenlyase subunit 4 (P16430)

Identification
Name:Formate hydrogenlyase subunit 4
Synonyms:
  • FHL subunit 4
  • Hydrogenase 3 component D
Gene Name:hycD
Enzyme Class:Not Available
Biological Properties
General Function:Involved in oxidation-reduction process
Specific Function:Specific function unknown
Cellular Location:Cell inner membrane; Multi-pass membrane protein
SMPDB Pathways:Not Available
KEGG Pathways:Not Available
EcoCyc Reactions:
1.0Thumb+1.0Thumb → 1.0Thumb+1.0Thumb
1.0Formic acid + 1.0Hydrogen ion → 1.0Carbon dioxide + 1.0Hydrogen (gas)
ReactionCard
Complex Reactions:
2.0Thumb+1.0Thumb+1.0Thumb → 1.0Thumb+2.0Thumb
2.0Hydrogen ion + 1.0Hydrogen (gas) + 1.0Ubiquinone-8 → 1.0Ubiquinol-8 + 2.0Hydrogen ion
ReactionCard
Metabolites:
ECMDB IDNameView
ECMDB04030Carbon dioxideMetaboCard
ECMDB00142Formic acidMetaboCard
ECMDB01362Hydrogen (gas)MetaboCard
ECMDB21225Hydrogen ionMetaboCard
ECMDB01060Ubiquinol-8MetaboCard
ECMDB21296Ubiquinone-8MetaboCard
GO Classification:
Component
cell part
membrane
Process
metabolic process
oxidation reduction
Gene Properties
Blattner:b2722
Gene OrientationCounterclockwise
Centisome Percentage:61.31
Left Sequence End2844511
Right Sequence End2845434
Gene Sequence:
>924 bp
GTGAAAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTA
CCGGTGCTGGCGGCAGCGTCTGACTGGACGTTACAAGGAGCCTGGTCGCCTACGCGCGCG
AAAGCCCTGCCAATTTGTGAAAGCTGGCGCATTCCTTATGCCGATTCGTTATCCAGCCTT
GCCGCCAGTTGCGATGCGGTTTTTGTGCATTCCAGCACCGCCAGCCACTTTGACGTGGTC
AGTACGTTACTCAATGCGGGGGTACATGTCTGTGTCGATAAACCGCTGGCAGAAAATCTG
CGCGATGCTGAACGGCTGGTGGAACTGGCGGCGCGGAAAAAACTGACGTTGATGGTCGGT
TTTAACCGTCGTTTCGCACCACTCTACGGTGAGTTAAAAACGCAACTCGCCACCGCAGCC
TCGCTAAGAATGGATAAACATCGTAGCAATAGCGTCGGGCCACACGATCTTTATTTCACG
TTGCTGGATGATTATCTGCATGTGGTGGATACCGCGCTGTGGTTGTCGGGCGGCAAAGCC
TCTCTGGATGGCGGTACGCTACTGACTAACGACGCTGGCGAAATGCTGTTTGCCGAGCAC
CATTTTTCGGCTGGTCCTTTGCAGATCACCACCTGTATGCATCGCCGTGCCGGAAGTCAG
CGTGAAACCGTGCAGGCCGTGACTGACGGTGCGCTCATCGACATTACGGATATGCGCGAA
TGGCGTGAGGAGCGCGGGCAGGGCGTAGTGCATAAACCGATTCCTGGTTGGCAGAGTACG
CTTGAGCAACGTGGGTTTGTCGGCTGTGCGCGGCACTTCATTGAATGTGTGCAAAACCAG
ACAGTTCCGCAAACCGCCGGCGAACAGGCCGTGCTGGCGCAACGTATCGTTGACAAGATC
TGGCGCGATGCGATGAGTGAATAA
Protein Properties
Pfam Domain Function:
Protein Residues:307
Protein Molecular Weight:33029
Protein Theoretical pI:7
Signaling Regions:
  • None
Transmembrane Regions:
  • 3-23
  • 68-88
  • 94-114
  • 132-152
  • 168-188
  • 222-242
  • 254-274
  • 285-305
Protein Sequence:
>Formate hydrogenlyase subunit 4
MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYRDIIKLLGRQSVGPDAS
GWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLYLFAIARFFFAISGLDTGS
PFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNITDTVYHWPLSQSIPLVLALC
ACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFGVMKWGISLKQLVVLQMFVGVFI
PWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFENSMARLRLDITPRITWAGFGFAFLA
FVSLLAA
References
External Links:
ResourceLink
Uniprot ID:P16430
Uniprot Name:HYCD_ECOLI
GenBank Gene ID:AP009048
Genebank Protein ID:4062647
Ecogene ID:EG10477
Ecocyc:EG10477
ColiBase:b2722
Kegg Gene:b2722
EchoBASE ID:EB0472
CCDB:HYCD_ECOLI
BacMap:16130629
General Reference:
  • Blattner, F. R., Plunkett, G. 3rd, Bloch, C. A., Perna, N. T., Burland, V., Riley, M., Collado-Vides, J., Glasner, J. D., Rode, C. K., Mayhew, G. F., Gregor, J., Davis, N. W., Kirkpatrick, H. A., Goeden, M. A., Rose, D. J., Mau, B., Shao, Y. (1997). "The complete genome sequence of Escherichia coli K-12." Science 277:1453-1462. Pubmed: 9278503
  • Bohm, R., Sauter, M., Bock, A. (1990). "Nucleotide sequence and expression of an operon in Escherichia coli coding for formate hydrogenlyase components." Mol Microbiol 4:231-243. Pubmed: 2187144
  • Daley, D. O., Rapp, M., Granseth, E., Melen, K., Drew, D., von Heijne, G. (2005). "Global topology analysis of the Escherichia coli inner membrane proteome." Science 308:1321-1323. Pubmed: 15919996
  • Hayashi, K., Morooka, N., Yamamoto, Y., Fujita, K., Isono, K., Choi, S., Ohtsubo, E., Baba, T., Wanner, B. L., Mori, H., Horiuchi, T. (2006). "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Mol Syst Biol 2:2006.0007. Pubmed: 16738553